Видовая диагностика возбудителей фитоплазмозов и фузариозов на западноевропейских сортах винограда

The results of molecular diagnostics phytoplasmas and fusariums on some grape varieties from different growing areas. In the samples of grape were detected two infections Candidatus Phytoplasma solani and five infections from genus Fusarium .Представлены результаты молекулярной диагностики фитоплазм и фузариумов на некоторых сортах винограда из различных мест произрастания. В образцах винограда выявлено два заражения фитоплазмой Candidatus Phytoplasma solani и пять образцов, зараженных грибами рода Fusarium

ВЫЯВЛЕНИЕ И ИДЕНТИФИКАЦИЯ ВОЗБУДИТЕЛЕЙ ФИТОПЛАЗМОЗОВ ГРУППЫ APPLE PROLIFERATION НА ПЛОДОВЫХ КУЛЬТУРАХ

In this research were studied the Apple proliferation group phytoplasmas (AP) and their diagnostics. Was estimated the distribution AP group phytoplasmas on the territory of the Russian Federation and European countries. All DNA samples were tested by nested-PCR with universal primers designed against the regions of the genes 16-23S. Were tested more than 60 samples of fruit trees, were detected AP group phytoplasmas. Candidatus Phytoplasma mali was detected in samples from Spain. Ca. Phytoplasma pyri was detected in samples from the Republic of Dagestan, Italy and Spain. Ca. Phytoplasma prunorum was detected in the samples from the Czech Republic. Obtained DNA sequences of regions of the apple proliferation group phytoplasmas were deposited into the National Center for Biotechnology Information (NCBI).Целью исследования было изучение, выявление и идентификация фитоплазмозов группы Apple proliferation (АР) на территории Российской Федерации и европейских стран. Из 67 исследованных образцов плодовых культур определены фитоплазмы, входящие в группу AP. Все образцы выделенной ДНК анализировали методом «nested»-ПЦР с помощью универсальных праймеров, разработанных для участка 16-23S генов. Показано, что возбудитель заболевания «пролиферация яблони» (AP) Candidatus Phytoplasma mali обнаружен в Испании, возбудитель заболевания «истощение груши» (PD) Candidatus Phytoplasma pyri — в Республике Дагестан, Италии, Испании, возбудитель заболевания «хлоротическое скручивание листьев абрикоса» (ESFY) Candidatus Phytoplasma prunorum — в Чехии. По результатам секвенирования получены и депонированы в Международную генетическую базу данных (NCBI) участки ДНК фитоплазм группы пролиферации яблони

ВЫЯВЛЕНИЕ И ИДЕНТИФИКАЦИЯ ВОЗБУДИТЕЛЕЙ ФИТОПЛАЗМОЗОВ ГРУППЫ APPLE PROLIFERATION НА ПЛОДОВЫХ КУЛЬТУРАХ

In this research were studied the Apple proliferation group phytoplasmas (AP) and their diagnostics. Was estimated the distribution AP group phytoplasmas on the territory of the Russian Federation and European countries. All DNA samples were tested by nested-PCR with universal primers designed against the regions of the genes 16-23S. Were tested more than 60 samples of fruit trees, were detected AP group phytoplasmas. Candidatus Phytoplasma mali was detected in samples from Spain. Ca. Phytoplasma pyri was detected in samples from the Republic of Dagestan, Italy and Spain. Ca. Phytoplasma prunorum was detected in the samples from the Czech Republic. Obtained DNA sequences of regions of the apple proliferation group phytoplasmas were deposited into the National Center for Biotechnology Information (NCBI).Целью исследования было изучение, выявление и идентификация фитоплазмозов группы Apple proliferation (АР) на территории Российской Федерации и европейских стран. Из 67 исследованных образцов плодовых культур определены фитоплазмы, входящие в группу AP. Все образцы выделенной ДНК анализировали методом «nested»-ПЦР с помощью универсальных праймеров, разработанных для участка 16-23S генов. Показано, что возбудитель заболевания «пролиферация яблони» (AP) Candidatus Phytoplasma mali обнаружен в Испании, возбудитель заболевания «истощение груши» (PD) Candidatus Phytoplasma pyri — в Республике Дагестан, Италии, Испании, возбудитель заболевания «хлоротическое скручивание листьев абрикоса» (ESFY) Candidatus Phytoplasma prunorum — в Чехии. По результатам секвенирования получены и депонированы в Международную генетическую базу данных (NCBI) участки ДНК фитоплазм группы пролиферации яблони

Апробация тест-систем для детекции фитоплазм яблони и груши

The article presents data on testing of kits for detection Apple proliferation group phytoplasmas (AP): Candidatus phytoplasma mali and Candidatus phyto-plasma pyri. Commercial kits are presented by company «AgroDiagnostica». Phytoplasmas DNA was analyzed be real-time polymerase chain reaction (qPCR).Представлены данные по апробации тест-систем на обнаружение фитоплазм Candidatus phytoplasma mali и Candidatus phytoplasma pyri группы Apple proliferation (AP). Коммерческие наборы представлены компанией ООО «АгроДиагностика», ДНК фитоплазм анализировали методом полимеразной цепной реакции (ПЦР) в режиме реального времени (ПЦР-РВ)

Апробация тест-систем для детекции фитоплазм яблони и груши

The article presents data on testing of kits for detection Apple proliferation group phytoplasmas (AP): Candidatus phytoplasma mali and Candidatus phyto-plasma pyri. Commercial kits are presented by company «AgroDiagnostica». Phytoplasmas DNA was analyzed be real-time polymerase chain reaction (qPCR).Представлены данные по апробации тест-систем на обнаружение фитоплазм Candidatus phytoplasma mali и Candidatus phytoplasma pyri группы Apple proliferation (AP). Коммерческие наборы представлены компанией ООО «АгроДиагностика», ДНК фитоплазм анализировали методом полимеразной цепной реакции (ПЦР) в режиме реального времени (ПЦР-РВ)

Definitions of antibodies to the antitumor enzyme L-lysine-α-oxidase, the study of its immunogenic properties and allergenic activity

The determination of antibodies to the antitumor enzyme L-lysine-α-oxidase Trichoderma harzianum Rifai F-180 was studied using enzyme-linked immunosorbent assay. It was shown that when testing the enzyme in mice and guinea pigs at a dose of 35 U / kg, the antitumor enzyme L-lysine-α-oxidase has low immunogenicity. Antibodies not only reduce catalytic activity, but also affect the affinity of the enzyme with the substrate. Guinea pigs showed that native and modified L-lysine-α-oxidase at a dose of 35 U / kg in vivo and in vitro has a weak allergenic activity

Definitions of antibodies to the antitumor enzyme l-lysine-α-oxidase, the study of its immunogenic properties and allergenic activity

he determination of antibodies to the antitumor enzyme L-lysine-α-oxidase Trichoderma har-zianum Rifai F-180 was studied using enzyme-linked immunosorbent assay. It was shown that when testing the enzyme in mice and guinea pigs at a dose of 35 U / kg, the antitumor enzyme L-lysine-α-oxidase has low immunogenicity. Antibodies not only reduce catalytic activity, but also affect the affinity of the enzyme with the substrate. Guinea pigs showed that native and modified L-lysine-α-oxidase at a dose of 35 U / kg in vivo and in vitro has a weak allergenic activity

Definitions of antibodies to the antitumor enzyme l-lysine-α-oxidase, the study of its immunogenic properties and allergenic activity [Definiciones de anticuerpos contra la enzima antitumoral l-lisina-α-oxidasa, estudio de sus propiedades inmunogénicas y actividad alergénica]

he determination of antibodies to the antitumor enzyme L-lysine-α-oxidase Trichoderma har-zianum Rifai F-180 was studied using enzyme-linked immunosorbent assay. It was shown that when testing the enzyme in mice and guinea pigs at a dose of 35 U / kg, the antitumor enzyme L-lysine-α-oxidase has low immunogenicity. Antibodies not only reduce catalytic activity, but also affect the affinity of the enzyme with the substrate. Guinea pigs showed that native and modified L-lysine-α-oxidase at a dose of 35 U / kg in vivo and in vitro has a weak allergenic activity. © 2021, Venezuelan Society of Pharmacology and Clinical and Therapeutic Pharmacology. All rights reserved

Characterization of L-Lysine-Α-Oxidase, A New Antitumor And Antiviral Drug Substance Synthesized By Trichoderma

The trigger to the studies of L-lysine-α-oxidase is of great interest for the scientists across the world. Regarding Russian Federation, the enzyme is under examination by several laboratories. Attracted by the polyfunctionality of the enzyme, the likelihood of utilizing this substance in the creation of multiple dosage forms of different therapeutic orientation. Thus, the main aim of the study is to analyze the new characteristics of L-lysine-α-oxidase as a new antitumor and antiviral drug substance synthesized by Trichoderma. To meet the study's aim, a biological experiments were carried out on guinea pigs weighing 200-250 g and CBA mice (CBA x C57BI) weighing 18-20 g, C57 BI, SHR.Native L-lysine-α-oxidase was injected into C57BI mice five times intravenously at a dose of 35 U/kg. Blood was sampled every week for four weeks from the beginning of immunization (n=7), and the obtained sera of mice were analyzed by enzyme-linked immunosorbent assay according to a previously developed method. The sera were titrated in increments of 2. Tests of L-lysine-α-oxidase in mice and guinea pigs found low immunogenicity of L-lysine-α-oxidase when administered in mice at a dose of 35 U/kg, or 0.8 ml of protein/kg. Based on the results obtained, antibodies not only reduce its catalytic activity but also affect the affinity of the enzyme with the substrate. Experiments on guinea pigs have shown that native and modified L-lysine-α-oxidase at a dose of 35 U/kg in vivo and in vitro has a low allergenic activity. The allergenic activity of L-lysine-α-oxidase was investigated in comparison with other drugs. The results od this article, can be considered as one of a brand-new and effective methods for assessing the body sensitization caused by the use of enzymes and can contribute to the development of the respective field