Evaluation of the Debye temperature for iron cores in human liver ferritin and its pharmaceutical analogue Ferrum Lek using Mossbauer spectroscopy

An iron polymaltose complex Ferrum Lek used as antianemic drug and considered as a ferritin analogue and human liver ferritin were investigated in the temperature range from 295K to 90K by means of 57Fe Mossbauer spectroscopy with a high velocity resolution i.e. in 4096 channels. The Debye temperatures equal to 502K for Ferrum Lek and to 461K for human liver ferritin were determined from the temperature dependence of the center shift obtained using two different fitting procedures.Comment: 13 pages, 5 figure

Comparative study of nanosized iron cores in human liver ferritin and its pharmaceutically important models Maltofer® and Ferrum Lek using Mössbauer spectroscopy

Studies of human liver ferritin and its pharmaceutically important models Maltofer® and Ferrum Lek were carried out using Mössbauer spectroscopy with a high velocity resolution at 295 and 90 K and Mössbauer spectroscopy with a low velocity resolution at 40 and 20 K. The Mössbauer spectra fits using a multi-component model confirm the hypothesis of the complicated heterogeneous structure of nanosized iron cores in the investigated samples. © 2013 Allerton Press, Inc

Structural and magnetic study of the iron cores in iron(III)-polymaltose pharmaceutical ferritin analogue Ferrifol®

Iron(III)-polymaltose pharmaceutical ferritin analogue Ferrifol® was investigated by high resolution transmission electron microscopy (HRTEM), X-ray diffraction, thermogravimetry, electron magnetic resonance (EMR) spectroscopy, dc magnetization measurements and 57Fe Mössbauer spectroscopy to get novel information about the structural arrangement of the iron core. The Ferrifol® Mössbauer spectra measured in the range from 295 to 90 K demonstrated non-Lorentzian two-peak pattern. These spectra were better fitted using a superposition of 5 quadrupole doublets with the same line width. The obtained Mössbauer parameters were different and an unusual line broadening with temperature decrease was observed. Measurements of the Ferrifol® Mössbauer spectra from 60 to 20 K demonstrated a slow decrease of magnetic relaxation in the iron core. Zero-field-cooled and field-cooled magnetization measurements revealed a blocking temperature at ~33 K and paramagnetic state of the Ferrifol® iron core at higher temperatures. Isothermal magnetization measurements at 5 K show that the saturation magnetic moment is ~0.31 emu/g. X-band EMR spectroscopy measurements revealed the presence of different magnetic species in the sample. Transmission electron microscopy demonstrated that the size of the iron cores in Ferrifol® is in the range 2–6 nm. The lattice periodicity in these iron cores, measured on the HRTEM images, appeared to be vary in the range 2.2–2.7 Å. This can be best understood as sets of close packed O(OH) layers in ferrihydrite cores without long range correlation

Study of the Iron State in Pharmaceutical Products Using Mössbauer Spectroscopy

This work is devoted to study of the iron state in iron-containing pharmaceuticals using Mössbauer spectroscopy. Obtained results showed useful application of Mössbauer spectroscopy for characterization of the iron state in various pharmaceutical products.The authors wish to thank Dr. I. N. Antsigin for useful comments and support. This work was supported by the Ministry of Science and Higher Education of the Russian Federation, project # FEUZ-2020-0060

Lysogenic System of Non-Epidemic Cholera Vibrio El Tor, Resistant to Diagnostic Bacteriophage CTX-

Studied are non-epidemic strains of V. cholerae O1 biovar El Tor taken from the National collection of pathogenic bacteria lodged at the Russian Research Anti-Plague Institute Microbe, which were not sensible to diagnostic cholera bacteriophage El Tor ctx- at the time of isolation. Represented are the results of investigation regarding a cause of a phage-resistance of these cultures using specialized test-system consisting of indicator strains. Identified is the carriage of temperate phages among 88,0 % of the cultures examined

Differences of the 57Fe hyperfine parameters in both oxyhemoglobin and spleen from normal human and patient with primary myelofibrosis

Study of oxyhemoglobin in red blood cells and spleen tissues from normal human and patient with primary myelofibrosis was carried out using Mössbauer spectroscopy with a high velocity resolution. The 57Fe hyperfine parameters were evaluated and small variations in quadrupole splitting were revealed for both normal human and patient's oxyhemoglobin and both normal human and patient's spleen. © 2012 Springer Science+Business Media Dordrecht

Structural and Magnetic Study of the Iron Cores in Iron(III)-Polymaltose Pharmaceutical Ferritin Analogue Ferrifol®

Iron(III)-polymaltose pharmaceutical ferritin analogue Ferrifol® was investigated by high resolution transmission electron microscopy (HRTEM), X-ray diffraction, thermogravimetry, electron magnetic resonance (EMR) spectroscopy, direct current magnetization measurements and 57Fe Mössbauer spectroscopy to get novel information about the structural arrangement of the iron core. The Ferrifol® Mössbauer spectra measured in the range from 295 K to 90 K demonstrated non-Lorentzian two-peak pattern. These spectra were better fitted using a superposition of 5 quadrupole doublets with the same line width. The obtained Mössbauer parameters were different and an unusual line broadening with temperature decrease was observed. Measurements of the Ferrifol® Mössbauer spectra from 60 K to 20 K demonstrated a slow decrease of magnetic relaxation in the iron core. Zero-field-cooled and field-cooled magnetization measurements revealed a blocking temperature at ~33 K and a paramagnetic state of the Ferrifol® iron core at higher temperatures. Isothermal magnetization measurements at 5 K show that the saturation magnetic moment is ~0.31 emu/g. X-band EMR spectroscopy measurements revealed the presence of different magnetic species in the sample. Transmission electron microscopy demonstrated that the size of the iron cores in Ferrifol® is in the range 2–6 nm. The lattice periodicity in these iron cores, measured on the HRTEM images, vary in the range 2.2–2.7 Å. This can be best understood as sets of close packed O(OH) layers in ferrihydrite cores without long range correlation. © 2020 Elsevier Inc.The authors wish to thank Prof. Ferenc Simon (Institute of Physics, Budapest University of Technology and Economics, Budapest, Hungary) for making available the applied spectrometer for recording the EMR spectra and Dr. A.V. Chukin (Institute of Physics and Technology, Ural Federal University, Ekaterinburg, Russian Federation) for XRD measurements. This work was supported by the Ministry of Science and Higher Education of the Russian Federation, project No FEUZ-2020-0060, and Act 211 of the Government of the Russian Federation, contract No 02.A03.21.0006. V.K.K. was supported by the János Bolyai Postdoctoral Fellowship of the Hungarian Academy of Sciences and the ÚNKP-19-4 New National Excellence Program of the Ministry for Innovation and Technology. HRTEM facility at the Centre for Energy Research was granted by the European Structural and Investment Funds, grant no. VEKOP-2.3.3-15-2016-00002. This work was in part supported by the Hungarian National Research, Development and Innovation Office – NKFIH (K115784, K115913 and K134770). This work was carried out within the Agreement of Cooperation between the Ural Federal University (Ekaterinburg) and the Eötvös Loránd University (Budapest)

Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C

New nutrient media based on baker yeast autolizate were used for the first time for manufacturing of diagnostic preparation of plague bacteriophage L-413C. Experimental media provide high concentration of phage particles at the stage of propagation, and good survivability in lyophilization. Media in which yeast autolizate was a nutrient protein basis appeared to be more effective than those in which it was a stimulating additive. Phage preparations preserved stability of properties during storage at 4-8 °С, and at a higher temperature in the test of accelerated aging. Introduction of yeast nutrient media in technology of plague diagnostic bacteriophage L-413C manufacturing opens good prospects for increasing of production efficiency and decreasing of cost value of the preparatio

Development and Main Stages of Introduction of the Preparation “Cholera O139 Diagnostic Fluorescent Immunoglobulins”

– for environmental objects. Application of the preparation for practical purposes was considered to be promising, and it was recommended for State registration as a product of medical application

Prospects for Application of Ultrafiltration Technology for the Scaled Preparation of Plague Microbe and Cholera Vibrio Major Antigens

Demonstrated is the possibility of application of ultrafiltration technologies in the process of cholera toxin and plague agent capsular antigen precipitation under production conditions. Application of ultrafiltration techniques permits of the reduction of losses at the stages of isolation and purification of antigen preparations; and concentration of raw material or semi-finished product provides for the reduction of labor inputs. Thus it leads to the increase in productivity and economical efficiency