Utilización de microhilos magnéticos para promover la muerte de células de osteosarcoma mediante hipertermia magnética in vitro

Los tratamientos para el cáncer que se llevan a cabo actualmente tienen una serie de limitaciones que, unido a que se trata de técnicas invasivas, hace que se tienda a buscar nuevas alternativas. Una de ellas es la hipertermia dirigida, cuyo objetivo son las células cancerígenas. Se trata de una técnica relativamente nueva, por lo que aún no se ha estudiado en profundidad. En este trabajo, se han utilizado microhilos magnéticos amorfos recubiertos de vidrio para calentar células de osteosarcoma aplicando un campo magnético alterno. De acuerdo con los resultados obtenidos de los ensayos realizados “in vitro”, se puede confirmar que la utilización de estos microhilos magnéticos reduce la proliferación de células cancerígenas dando lugar finalmente a la muerte celular. Además, esta técnica es un proceso mucho menos invasivo que los utilizados actualmente y que requiere exposiciones breves a campos magnéticos débiles. Por todo esto, la hipertermia dirigida se presenta como una alternativa efectiva para el tratamiento de osteosarcoma.Por último, agradecer a Tamag Ibérica S.L. por proporcionarnos los microhilos magnéticos para poder llevar a cabo este estudio y a Fomento de San Sebastián por financiar el proyecto de investigació

TARTESSUS: A customized electrospun drug delivery system loaded with Irinotecan for Local and sustained chemotherapy release in pancreatic cancer

Post-surgical chemotherapy in pancreatic cancer has notorious side effects due to the high dose required. Multiple devices have been designed to tackle this aspect and achieve a delayed drug release. This study aimed to explore the controlled and sustained local delivery of a reduced drug dose from an irinotecan-loaded electrospun nanofiber membrane (named TARTESSUS) that can be placed on the patients' tissue after tumor resection surgery. The drug delivery system formulation was made of polycaprolactone (PCL). The mechanical properties and the release kinetics of the drug were adjusted by the electrospinning parameters and by the polymer ratio between 10 w.t.% and 14 w.t.% of PCL in formic acid:acetic acid:chloroform (47.5:47.5:5). The irinotecan release analysis was performed and three different release periods were obtained, depending on the concentration of the polymer in the dissolution. The TARTESSUS device was tested in 2D and 3D cell cultures and it demonstrated a decrease in cell viability in 2D culture between 72 h and day 7 from the start of treatment. In 3D culture, a decrease in viability was seen between 72 h, day 7 (p < 0.001), day 10 (p < 0.001), 14 (p < 0.001), and day 17 (p = 0.003) as well as a decrease in proliferation between 72 h and day 10 (p = 0.030) and a reduction in spheroid size during days 10 (p = 0.001), 14 (p < 0.001), and 17 (p < 0.001). In conclusion, TARTESSUS showed a successful encapsulation of a chemotherapeutic drug and a sustained and delayed release with an adjustable releasing period to optimize the therapeutic effect in pancreatic cancer treatment

Utilización de microhilos magnéticos para promover la muerte de células de osteosarcoma mediante hipertermia magnética in vitro

Los tratamientos para el cáncer que se llevan a cabo actualmente tienen una serie de limitaciones que, unido a que se trata de técnicas invasivas, hace que se tienda a buscar nuevas alternativas. Una de ellas es la hipertermia dirigida, cuyo objetivo son las células cancerígenas. Se trata de una técnica relativamente nueva, por lo que aún no se ha estudiado en profundidad. En este trabajo, se han utilizado microhilos magnéticos amorfos recubiertos de vidrio para calentar células de osteosarcoma aplicando un campo magnético alterno. De acuerdo con los resultados obtenidos de los ensayos realizados “in vitro”, se puede confirmar que la utilización de estos microhilos magnéticos reduce la proliferación de células cancerígenas dando lugar finalmente a la muerte celular. Además, esta técnica es un proceso mucho menos invasivo que los utilizados actualmente y que requiere exposiciones breves a campos magnéticos débiles. Por todo esto, la hipertermia dirigida se presenta como una alternativa efectiva para el tratamiento de osteosarcoma.Por último, agradecer a Tamag Ibérica S.L. por proporcionarnos los microhilos magnéticos para poder llevar a cabo este estudio y a Fomento de San Sebastián por financiar el proyecto de investigació

Glass-coated ferromagnetic microwire-induced magnetic hyperthermia for in vitro cancer cell treatment

Limitations in effectiveness and the invasive nature of current cancer treatment options emphasize the need for further clinical advancements. Among other approaches, targeted hyperthermia is as a new strategy aimed at targeting cancerous cells to improve the efficacy of radiotherapy or cytotoxic drugs. However, the testing of magnetic vehicles has mainly focused on the use of nanoparticles. In this work, Fe77B10Si10C3 glass-coated amorphous magnetic microwires were assessed for the first time as magnetic vehicles with high potential for the localized heating of osteosarcoma cells by means of an AC magnetic field. The results from the in vitro assays performed inside a microfluidic device demonstrated the ability of these magnetic microwires to induce malignant cell death. Exposing the system to different magnetic fields for less than 1 h provoked a reduction up to 89% of the osteosarcoma cell population, whereas healthy myoblastoma cells remained nearly unaffected. The proposed technology demonstrates in vitro the effectiveness of these microwires as vehicles for targeted magnetic hyperthermia

Glass-coated ferromagnetic microwire-induced magnetic hyperthermia for in vitro cancer cell treatment

Limitations in effectiveness and the invasive nature of current cancer treatment options emphasize the need for further clinical advancements. Among other approaches, targeted hyperthermia is as a new strategy aimed at targeting cancerous cells to improve the efficacy of radiotherapy or cytotoxic drugs. However, the testing of magnetic vehicles has mainly focused on the use of nanoparticles. In this work, Fe77B10Si10C3 glass-coated amorphous magnetic microwires were assessed for the first time as magnetic vehicles with high potential for the localized heating of osteosarcoma cells by means of an AC magnetic field. The results from the in vitro assays performed inside a microfluidic device demonstrated the ability of these magnetic microwires to induce malignant cell death. Exposing the system to different magnetic fields for less than 1 h provoked a reduction up to 89% of the osteosarcoma cell population, whereas healthy myoblastoma cells remained nearly unaffected. The proposed technology demonstrates in vitro the effectiveness of these microwires as vehicles for targeted magnetic hyperthermia