Genetics of Triatomine Bugs (Family Reduviidae) in Relation to their Role as Vectors of Chagas' Disease, Trypanosomal Infection of the Americas

Trypanosomiasis caused by Trypanosoma cruzi and transmitted by triatomine bugs is widespread in South and Central America, human Infections, chronic or acute, being known as Chagas' disease. Since blood forms of the trypanosome in man and animals are commonly too few to be found in blood films, conclusive evidence of infection is still by xenodiagnosis. This requires vector bugs to be engorged on a suspected infected vertebrate and the bug faeces examined a month or so later by which time the trypanosomes should have greatly multiplied in the insect gut. It is known that there is variation between triatomine species and between individuals within species in ability to develop T. cruzi infections. The present research investigated the genetic basis of this variable susceptibility in one species, Rhodnius prolixus, by selection for susceptible and refractory bug populations and revealed evidence of polygenic control of susceptibility to infection with T. cruzi; this contrasts with the major gene mechanisms reported for other pathogens in other insect vectors. Male bugs showed significantly higher levels of infection than females and may be more efficient for xenodiagnosis. The radioresistance of triatomine bugs may be related to their chromosome morphology. Male R. prolixus given a sub-sterilising irradiation dose were less sterile than their progeny, this delay resulting from the diffuse structure of the centromeres of triatomine chromosome. These results are discussed in relation 10 possible control of triatomines by genetic manipulation. Investigation of spermatogenesis by autoradiographic techniques male R. prolixus revealed that starvation induced diapause inhibits spermiogenesis. A blood-borne factor produced when the diapausing bug is fed may directly affect the rate of mulosis. The karyotype is described for several triatomine species. A method was developed for distinguishing between individual mitotic chromosomes within the complement of Triatomi infostan., and K. prolixus. the chromosome markers being valuable for further genetic studies of triatomine bugs

Identification of human-infective trypanosomes in animal reservoir of sleeping sickness in Uganda by means of serum-resistance-associated (SRA) gene.

BACKGROUND: The expansion of sleeping sickness caused by Trypanosoma brucei rhodesiense beyond its traditional focus in southeast Uganda has been linked with large-scale livestock restocking. To assess the risk presented to the human population by domestic livestock, human-infective T b rhodesiense must be distinguished from non-human-infective T brucei brucei, since both parasites can be present in cattle. We investigated the use of a simple genetic marker to characterise parasites collected from cattle in villages within the new sleeping sickness focus in Soroti District, Uganda. METHODS: 70 T brucei sl samples of known human infectivity status collected from human beings and cattle in Tororo District, Uganda, from 1989 to 1991 were screened for the presence of the human-serum-resistance-associated (SRA) gene by conventional PCR. In 2000-01, blood samples from 200 randomly selected cattle in six villages and two markets in Soroti District were screened for T brucei sl parasites by PCR; positive samples were screened for the presence of the SRA gene. FINDINGS: The SRA gene was present in all 29 samples from patients with sleeping sickness in Tororo District. Of the 41 samples collected from cattle at the same time, the SRA gene was present in the eight samples that tested resistant to human serum in vitro, whereas it was absent from all 33 isolates that were sensitive to human serum in vitro. Of the 200 cattle sampled in Soroti District, we estimated that up to 18% (95% CI 12-23) were infected with T b rhodesiense. INTERPRETATION: Detection of the SRA gene could provide the basis for a simple diagnostic test to enable targeted control of T b rhodesiense in the domestic livestock reservoir, thereby reducing the public-health burden of sleeping sickness in east Africa

Controlling sleeping sickness - a review

Following a period characterized by severe epidemics of sleeping sickness, restoration of effective control and surveillance systems has raised the question of eliminating the disease from sub-Saharan Africa. Given sufficient political and financial support, elimination is now considered a reasonable aim in countries reporting zero or less than 100 cases per year. This success may lead health authorities across the affected region to downgrade the disease from ‘neglected' to simply being ignored. In view of the significant levels of under-reporting of sleeping sickness mortality in rural communities, this could be a short-sighted policy. Loss of capacity to deal with new epidemics, which can arise as a consequence of loss of commitment or civil upheaval, would have serious consequences. The present period should be seen as a clear opportunity for public-private partnerships to develop simpler and more cost-effective tools and strategies for sustainable sleeping sickness control and surveillance, including diagnostics, treatment and vector contro

Non-Locality and Theories of Causation

The aim of the paper is to investigate the characterization of an unambiguous notion of causation linking single space-llike separated events in EPR-Bell frameworks. This issue is investigated in ordinary quantum mechanics, with some hints to no collapse formulations of the theory such as Bohmian mechanics.Comment: Presented at the NATO Advanced Research Workshop on Modality, Probability and Bell's Theorems, Cracow, Poland, August 19-23, 200

Factors Affecting Trypanosome Maturation in Tsetse Flies

Trypanosoma brucei brucei infections which establish successfully in the tsetse fly midgut may subsequently mature into mammalian infective trypanosomes in the salivary glands. This maturation is not automatic and the control of these events is complex. Utilising direct in vivo feeding experiments, we report maturation of T. b. brucei infections in tsetse is regulated by antioxidants as well as environmental stimuli. Dissection of the maturation process provides opportunities to develop transmission blocking vaccines for trypanosomiasis. The present work suggests L-cysteine and/or nitric oxide are necessary for the differentiation of trypanosome midgut infections in tsetse

Non-realism : deep thought or a soft option ?

The claim that the observation of a violation of a Bell inequality leads to an alleged alternative between nonlocality and non-realism is annoying because of the vagueness of the second term.Comment: 5 page

Patterns in Age-Seroprevalence Consistent with Acquired Immunity against Trypanosoma brucei in Serengeti Lions

Trypanosomes cause disease in humans and livestock throughout sub-Saharan Africa. Although various species show evidence of clinical tolerance to trypanosomes, until now there has been no evidence of acquired immunity to natural infections. We discovered a distinct peak and decrease in age prevalence of T. brucei s.l. infection in wild African lions that is consistent with being driven by an exposure-dependent increase in cross-immunity following infections with the more genetically diverse species, T. congolense sensu latu. The causative agent of human sleeping sickness, T. brucei rhodesiense, disappears by 6 years of age apparently in response to cross-immunity from other trypanosomes, including the non-pathogenic subspecies, T. brucei brucei. These findings may suggest novel pathways for vaccinations against trypanosomiasis despite the notoriously complex antigenic surface proteins in these parasites

Bell Inequalities with Auxiliary Communication

What is the communication cost of simulating the correlations produced by quantum theory? We generalize Bell inequalities to the setting of local realistic theories augmented by a fixed amount of classical communication. Suppose two parties choose one of M two-outcome measurements and exchange 1 bit of information. We present the complete set of inequalities for M = 2, and the complete set of inequalities for the joint correlation observable for M = 3. We find that correlations produced by quantum theory satisfy both of these sets of inequalities. One bit of communication is therefore sufficient to simulate quantum correlations in both of these scenarios.Comment: 5 page