93 research outputs found
Gene expression of Solea senegalensis Kaup, 1858 fed with the synbiotic composed by sodic alginate and the probiotic Shewanella putrefaciens Pdp11
CURRENT-USE PESTICIDES AND PERSONAL CARE PRODUCTS IN SURFACE SEDIMENTS FROM THE SPANISH MEDITERRANEAN CONTINENTAL SHELF
Benefits of probiotic administration on growth and performance along metamorphosis and weaning of Senegalese sole (Solea senegalensis)
Suitable nutrition at first feeding in marine fish larvae is an important factor for successful larval and juvenile
rearing. In this way probiotic supplementation may contribute to better face the habitual stress of metamorphosis
and weaning at early sole stages. The aimof this study was to optimize Shewanella putrefaciens Pdp11 administration
to Senegalese sole (Solea senegalensis) at early stages of development. For this purpose, we studied the
influence of a short pulse of S. putrefaciens Pdp11 (10–30 dah) on growth performance, gut microbiota modulation,
digestive enzymes activities and body composition. These probiotic bacteria were incorporated using
Artemia as live vector (2.5 × 10
7
cfu mL
−1
) and supplied to sole specimens in a co-feeding regime (Gemma,
Skretting) by triplicate. S. putrefaciens Pdp11 colonized larval gut being present from23 to 119 dah. Probiotic supply
significantly modulated larval and fry gut microbiota. A PCA analysis including all the parameters analyzed
strongly associated S. putrefaciens Pdp11 presence with a higher fish growth, a higher digestive proteolytic activity
level and a fish body composition modulation along S. senegalensis rearing. In addition less size variability was
obtained frommetamorphosis until the end of weaning. In conclusion a short pulse of S. putrefaciens Pdp11 (10–
30 dah) seems sufficient to obtain a suitable microbial modulation involved in a better growth performance and
body composition that suggests a great potential for sole aquaculture production.En prens
Use of in vivo-induced antigen technology to identify bacterial genes expressed during solea senegalensis infection with Photobacterium damselae subsp. piscicida
The marine fish pathogen Photobacterium damselae subsp. piscicida (Phdp) is responsible for important outbreaks affecting several fish species including flatfish Solea senegalensis. The aim of this work was to identify in vivo-induced expressed immunogenic proteins using pooled sera from fish that have experienced photobacteriosis. In vivo induced immunogenic proteins included inosine-5'-monophosphate dehydrogenase (Impdh) and alkyl hydroperoxide reductase (AhpC), two proteins involved in peptide synthesis: serine hydroxymethyl transferase (Shmt) and alanyl-tRNA synthetase (AlaRS) and the non-ribosomal peptide synthetase involved in the synthesis of the siderophore piscibactin (Irp2)
Modulation of the intestinal microbiota and expression of gene of senegalese sole (Solea senegalensis, Kaup 1858) after dietary administration of oxytetracycline.
Oxitetracycline and probiotics (Pdp11) administration to sole juveniles showed important changes in the species composition of intestinal microbiota that established a relationship between these species and gene expression
Effect of two different pulses of Shewanella putrefaciens Pdp11 on Solea senegalensis larvae performance
Changes in intestinal microbiota and gene expression of immune system parameters caused by dietary administration of alginate and Shewanella putrefaciens Pdp11 in Senegalese sole (Solea senegalensis Kaup, 1858) specimens
Dietary administration of sodium alginate single or mixed with Pdp11 modulated the intestinal microbiota diversity of sole juveniles. Both treatments promoted a change in the species present in the gut in comparison with control group. In addition, oral administration of alginate promoted the appearance of an intestinal microbiota highly influenced by the prebiotic presence. Synbiotic administration of alginate with Pdp11 promoted a more pronounced decreasing in the similarity index and in the species richness. Both treatments modulated the expression of genes related with immune system functions
The treatment with the probiotic Shewanella putrefaciens Pdp11 of sepecimens of Solea senegalensis exposed to high stocking densities to enhance their resistance to disease
Aquaculture industry exposes fish to acute stress events, such as high stocking density, and a link between
stress and higher susceptibility to diseases has been concluded. Several studies have demonstrated
increased stress tolerance of fish treated with probiotics, but the mechanisms involved have not
been elucidated. Shewanella putrefaciens Pdp11 is a strain isolated from healthy gilthead seabream
(Sparus aurata L.) and it is considered as probiotics. The aim of this study was to evaluate the effect of the
dietary administration of this probiotics on the stress tolerance of Solea senegalensis specimens farmed
under high stocking density (PHD) compared to a group fed a commercial diet and farmed under the
same conditions (CHD). In addition, during the experiment, a natural infectious outbreak due to Vibrio
species affected fish farmed under crowding conditions. Changes in the microbiota and histology of
intestine and in the transcription of immune response genes were evaluated at 19 and 30 days of the
experiment. Mortality was observed after 9 days of the beginning of the experiment in CHD and PHD
groups, it being higher in the CHD group. Fish farmed under crowding stress showed reduced expression
of genes at 19 day probiotic feeding. On the contrary, a significant increase in immune related gene
expression was detected in CHD fish at 30 day, whereas the gene expression in fish from PHD group was
very similar to that showed in specimens fed and farmed with the conventional conditions. In addition,
the dietary administration of S. putrefaciens Pdp11 produced an important modulation of the intestinal
microbiota, which was significantly correlated with the high number of goblet cells detected in fish fed
the probiotic diet.Postprin
Probiotic Shewanella putrefaciens Pdp11 enhances stress resistance in just weaned Solea senegalensis fry
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