Transfer of dietary fatty acids from butyric acid fortified canola oil into the meat of broilers

The literature reported positive beneficial effects of butyric acid and canola oil on production performance traits of broiler chickens. Three hundred hybrid Ross 708 (150 males and 150 females) were randomly allotted to 10 pens per treatment with 5 males and 5 females per pen. Ten pens were administered a diet supplemented with soybean oil (control), ten pens the same basal diet but supplemented with a blend of mono-, di-, tri-glyceride of butyric acid added to soybean oil (T1) and ten pens the same basal diet supplemented with a mix of soybean and canola oil containing butyrate (T2). No differences in final body weight, dressing percentage, liver and thigh weight were found between groups. The T2 birds showed the highest feed/gain ratio (P<0.05). The control group showed the highest value for breast weight while the highest quantity of abdominal fat was in T2 carcasses. Fatty acid profile was significantly influenced by the presence of oil supplements, not only quantitatively but also qualitatively

Quality traits of Procambarus clarkii (girard) related to sex and refrigerated storage

The red swamp crayfish (Procambarus clarkii, Girard) is the most widespread alien species in Europe. Imported from Louisiana to southern Spain in 1973, where at present it is reared in crayfish farm and also diffused in the wild, it was introduced into Italy in the '80s and is now well established in natural environments of northern and central Italy. Naturalization of this alien species poses risks for aquatic ecosystems and its use as food may be a way to limit its spreading. The aim of this study was to analyse the morphological, mercantile and biochemical characteristics of male and female specimens of Procambarus clarkii and to evaluate changes in qualitative traits during refrigerated storage. Specimens of red swamp crayfish collected in a river, after a rearing period of 85 days in tanks, were harvested, killed by hypothermia and refrigerated (1°C). Male (n=31) and female (n=26) specimens were separated and measured for total body and abdomen length, 1 (n=10), 3 (n=16), 8 (n=16) and 10 days (n=15) after harvesting. They were weighed, sectioned to separate claws and tails (shell-on), which were weighed individually and their colour parameters (lightness, L*; chromaticity indexes, a* and b*) measured in dorsal and ventral sites. A pooled sample consisting of tail muscle of 2-3 specimens having the same storage times was homogenized and analysed for total lipids, qualitative and quantitative fatty acid composition and malondialdehyde (MDA). The other tails (shell-on) were steamed, the exoskeleton removed and abdominal muscles (shell-off tails) analysed for colour parameters in dorsal and ventral sites, and for free water (by compression method). Data was analysed by GLM using the SAS Package, with sex, storage time and relative interaction (except for data of chemical analysis) as fixed effects. Females and males differed in total (p<0.05) and abdominal (p<0.01) length (8.65 vs 8.27cm and 4.32 vs 4.04cm, respectively) and in body weight (corrected for cheliped weight, due their lack in some cases; 16.35 vs 13.89g; p<0.05), however the cooked-meat yield of shell-on tails was similar (46.53 vs 42.93%; p=0.19). Regarding colour parameters, the two sexes only differed in L*, which was significantly greater in males in the different sites of measurement, except the ventral site of raw shell-on tails. Males and females showed a similar water holding capacity of abdominal meat (mean 10.78±6.33cm2). No differences between the sexes were recorded for total lipids (mean 1.05±0.14%), MDA content and fatty acid profile (means SFA: 22.16%, MUFA: 28.40%, PUFAn6: 20.39%; PUFAn3: 28.80%; EPA: 18.24%; DHA: 7.17%). During refrigerated storage, a significant decrease in water holding capacity of muscle (free water increasing from 14.07 to 26.15cm2 on days 1 and 10, respectively; p<0.001) and in cooked-meat yield (from 50.92 to 39.11% on days 1 and 10, respectively; p<0.01) and sharp changes in cooked tail muscle colour parameters were noticed. The latter body part showed a significant decrease (p<0.001) in a* and b* at the dorsal site and in L* at the ventral site (77.74 and 49.14 on days 1 and 10, respectively) with increasing storage. During the refrigerated storage of the whole crayfish the changes recorded for the examined parameters indicate the quality decay, particularly after the 3rd day

Monobutirryne: a novel feed additive in the diet of broiler chickens.

Purpose of the present work was to validate the monoglyceride of butyric acid (MB) as an alternative to antibiotics as growth promoters in the diet of broiler chickens. The approach is a kind of prolongation of previous works, in which a blend of mono-, di- and tri-glycerides of butyric acid have been previously tested. The results indicated that MB was very efficient in limiting the mortality of birds challenged with Eimeria spp., but did not appreciably impair the performance of unchallenged birds. In conclusion, the metabolisable energy content of MB appeared comparable with that of soybean oil and MB a reliable coccidiostat

Morphological, nutritional and safety traits of bluefin tuna (Thunnus thynnus) reared in floating cages

To evaluate the influence of fattening on morphological, nutritional and safety characteristics, two lots of bluefin tuna were sampled before (November: 2 C, 4 ?) and after 5 months of fattening (April: 5 C, 5 ?). The specimens, after death, were refrigerated and analysed at different times over the period of a week for morphological and flesh physico-chemical parameters in six muscle sites. Tuna sampled in April had greater body weight (44.04 vs 36.41kg), trunk length (52.21 vs 48.22cm) and minimum and maximum circumferences (13.74 vs 12.77 and 94.90 vs 89.10cm). No differences in other linear measures or body components were found. Fattening did not influence flesh colour or total lipid content, producing small differences in its chemical composition: greater C18:0, C18:1n9 and PUFAn6 percentages; higher putrescine and histamine (0.489 vs 0.335 and 0.666 vs 0.370mg/kg) but lower spermine and spermidine (10.598 vs 17.387 and 2.420 vs 3.928mg/kg) levels. Large differences in physico-chemical parameters were found between muscle sites and a significant interaction between sampling date and muscle site indicated non homogeneous changes in chemical composition of dorsal, ventral and red muscle after fattening. Sex only influenced fat content in viscera (C 12.84 vs ? 9.84%)

Like the back of my hand: Visual ERPs reveal a specific change detection mechanism for the bodily self

The ability to identify our own body is considered a pivotal marker of self-awareness. Previous research demonstrated that subjects are more efficient in the recognition of images representing self rather than others' body effectors (self-advantage). Here, we verified whether, at an electrophysiological level, bodily-self recognition modulates change detection responses. In a first EEG experiment (discovery sample), event-related potentials (ERPs) were elicited by a pair of sequentially presented visual stimuli (vS1; vS2), representing either the self-hand or other people's hands. In a second EEG experiment (replicating sample), together with the previously described visual stimuli, also a familiar hand was presented. Participants were asked to decide whether vS2 was identical or different from vS1. Accuracy and response times were collected. In both experiments, results confirmed the presence of the self-advantage: participants responded faster and more accurately when the self-hand was presented. ERP results paralleled behavioral findings. Anytime the self-hand was presented, we observed significant change detection responses, with a larger N270 component for vS2 different rather than identical to vS1. Conversely, when the self-hand was not included, and even in response to the familiar hand in Experiment 2, we did not find any significant modulation of the change detection responses. Overall our findings, showing behavioral self-advantage and the selective modulation of N270 for the self-hand, support the existence of a specific mechanism devoted to bodily-self recognition, likely relying on the multimodal (visual and sensorimotor) dimension of the bodily-self representation. We propose that such a multimodal self-representation may activate the salience network, boosting change detection effects specifically for the self-hand

Effect of chestnut tannins and short chain fatty acids as anti-microbials and as feeding supplements in broilers rearing and meat quality

Chestnut tannins (CT) and saturated short medium chain fatty acids (SMCFA) are valid alternatives to contrast the growth of pathogens in poultry rearing, representing a valid alternative to antibiotics. However, the effect of their blends has never been tested. Two blends of CT extract and Sn1-monoglycerides of SMCFA (SN1) were tested in vitro against the proliferation of Clostridium perfringens, Salmonella typhymurium, Escherichia coli, Campylobacter jejuni. The tested concentrations were: 3.0 g/kg of CT; 3.0 g/kg of SN1; 2.0 g/kg of CT and 1.0 g/kg of SN1; 1.0 g/kg of CT and 2.0 g/kg of SN1. Furthermore, their effect on broiler performances and meat quality was evaluated in vivo: one-hundred Ross 308 male birds were fed a basal diet with no supplement (control group) or supplemented with CT or SN1 or their blends at the same concentration used in the in vitro trial. The in vitro assay confirmed the effectiveness of the CT and SN1 mixtures in reducing the growth of the tested bacteria while the in vivo trial showed that broiler performances, animal welfare and meat quality were not negatively affected by the blends, which could be a promising alternative in replacing antibiotics in poultry production

Quality traits of Procambarus clarkii (Girard) related to sex and refrigerated storage

The red swamp crayfish (Procambarus clarkii, Girard) is the most widespread alien species in Europe. Imported from Louisiana to southern Spain in 1973, where at present it is reared in crayfish farm and also diffused in the wild, it was introduced into Italy in the \u201980s and is now well established in natural environments of northern and central Italy. Naturalization of this alien species poses risks for aquatic ecosystems and its use as food may be a way to limit its spreading. The aim of this study was to analyse the morphological, mercantile and biochemical characteristics of male and female specimens of Procambarus clarkii and to evaluate changes in qualitative traits during refrigerated storage. Specimens of red swamp crayfish collected in a river, after a rearing period of 85 days in tanks, were harvested, killed by hypothermia and refrigerated (1\ub0C). Male (n=31) and female (n=26) specimens were separated and measured for total body and abdomen length, 1 (n=10), 3 (n=16), 8 (n=16) and 10 days (n=15) after harvesting. They were weighed, sectioned to separate claws and tails (shell-on), which were weighed individually and their colour parameters (lightness, L*; chromaticity indexes, a* and b*) measured in dorsal and ventral sites. A pooled sample consisting of tail muscle of 2-3 specimens having the same storage times was homogenized and analysed for total lipids, qualitative and quantitative fatty acid composition and malondialdehyde (MDA). The other tails (shell-on) were steamed, the exoskeleton removed and abdominal muscles (shell-off tails) analysed for colour parameters in dorsal and ventral sites, and for free water (by compression method). Data was analysed by GLM using the SAS Package, with sex, storage time and relative interaction (except for data of chemical analysis) as fixed effects. Females and males differed in total (p<0.05) and abdominal (p<0.01) length (8.65 vs 8.27cm and 4.32 vs 4.04cm, respectively) and in body weight (corrected for cheliped weight, due their lack in some cases; 16.35 vs 13.89g; p<0.05), however the cooked-meat yield of shell-on tails was similar (46.53 vs 42.93%; p=0.19). Regarding colour parameters, the two sexes only differed in L*, which was significantly greater in males in the different sites of measurement, except the ventral site of raw shell-on tails. Males and females showed a similar water holding capacity of abdominal meat (mean 10.78\ub16.33cm2). No differences between the sexes were recorded for total lipids (mean 1.05\ub10.14%), MDA content and fatty acid profile (means SFA: 22.16%, MUFA: 28.40%, PUFAn6: 20.39%; PUFAn3: 28.80%; EPA: 18.24%; DHA: 7.17%). During refrigerated storage, a significant decrease in water holding capacity of muscle (free water increasing from 14.07 to 26.15cm2 on days 1 and 10, respectively; p<0.001) and in cooked-meat yield (from 50.92 to 39.11% on days 1 and 10, respectively; p<0.01) and sharp changes in cooked tail muscle colour parameters were noticed. The latter body part showed a significant decrease (p<0.001) in a* and b* at the dorsal site and in L* at the ventral site (77.74 and 49.14 on days 1 and 10, respectively) with increasing storage. During the refrigerated storage of the whole crayfish the changes recorded for the examined parameters indicate the quality decay, particularly after the 3rd day