Identification and Prevalence of Aspergillus sp Isolated from Bali Dog’s Skin

Aspergillus sp are fungi that commonly caused a systemic respiratory infection in Human and Dog. In Bali, most of the dog are free-roaming dogs, and has poor skin health condition. Some of them are suspected with fungal infection. Aspergillus sp  has high risk to infect the dog, because they are available everywhere in environment. Therefore, this research is conducted for adding primary data of Aspergillus sp isolated from dog’s skin and their prevalence. The samples are 15 free-roaming Bali dogs with suspected fungal infection. Identification was carried out by using microscopic and macroscopic observation methods, skin swab was taken from the dog’s skin using the Mackenzie method, and the sterile toothbrush swab gently from the dog’s skin to the Sabouraud Dextrose Agar (SDA) media. The fungi were cultured on SDA media for macroscopic identification for approximately 3-4 days, mostly to observe how the fungal colony grew and its color. Microscopic identification are using adhesive tape, the tape was gently pressed into the fungal colony, the tape was stuck into the surface of object-glass with 3-4 drops of Methylene Blue staining, and the object glass was examined by using a light microscope, the findings of Aspergillus sp fungi are recorded and the data are descriptively presented. The result shows that most Aspergillus species that were found is Aspergillus fumigatus and Aspergillus niger according to their colonies growth and microscopic appearance, and the prevalence from 15 samples were 53.33%.    Keywords: Aspergillus sp, fungi, Bali Dogs, Identification, Prevalenc

ALBUMIN LEVELS OF BALI CATTLE THAT INFECTED BY FASCIOLA GIGANTICA

Albumin is a plasma protein that is most widely in the body and serves to maintain the osmotic pressure of blood. Albumin levels on Bali cattle are infected by various factors, one of which is a worm infection Fasciola gigantica. The purpose of this research is to determine the levels of albumin Bali cattle that infected by Fasciola gigantica. This research is using 60 blood samples of Bali cattle, consisting of 30 blood samples Bali cattle that infected by Fasciola gigantica and 30 blood samples of healthy Bali cattle. This research are using cross-sectional studies methods, samples were taken at the Slaughter House Pesanggaran, Denpasar. Analysis of the data is using the onesample t test, comparing samples that infected with the healthy samples. The results showed that the average levels of albumin Bali cattle infected with Fasciola gigantica was 3.24 g/dL and the average albumin levels were not infected with Fasciola gigantica was 4.14 g/dL. The data analysis showed that the infection of Fasciola gigantica highly significant (P<0.01) towards decreased levels of albumin Bali cattle at 0.9 g/dL. It is concluded that infected Bali cows that are slaughtered in the Slaughterhouse Pesanggaran suffered liver disfunction

THE OVICIDAL EFFECT OF ALBENDAZOLE AGAINST WORM EGGS OF PARAMPHISTOMUM SPP. BY IN-VITRO

Albendazole is one of the modern anthelmintics that has effect vermicidal, larvicidal, and ovicidal. The sample used is the eggs of worms Paramphistomum spp., obtained from the rumen of Bali cattle. This research is an experimental research laboratory and using complete random design. This study uses four different treatments with five repetitions so retrieved 20 types of worm eggs of Paramphistomum spp. research methods include the beginning of a collection of egg worm of Paramphistomum spp., the dose given that is 0,06 mL Albendazole/40mL NaCl (P1), 0,12mL Albendazole /40mL NaCl (P2), 0,24 mL Albendazole/40 mL NaCl (P3) and control without treatment (P0). The eggs were observed on the 10th day and the 30th day, and then counting the number of eggs that do not hatch. The data obtained were tested statistically with ANOVA test and proceed with Duncan test to see the differences between the treatments. Albendazole has ovicidal effect against the worm eggs of Paramphistomum spp. by invitro. The doses of 0,24mL Albendazole/40mL NaCl (P3) give the highest percentage number worm eggs of Paramphistomum spp. are not hatching with 16.85%.

OVICIDAL EFFECT WUDANI LEAF EXTRACT AGAINST EGGS FASCIOLA GIGANTICA WORM BY IN-VITRO

Bali cattle is a source of meat that has high economic value. One endoparasite disease that attacks cattle and very detrimental to farmers is Fascioliasis caused by Fasciola gigantica worm. This study aims to determine the ovicidal effect of the extract of wudani leaf against Fasciola gigantica worm eggs in-vitro and determine the effective dose of it. This study used a completely randomized design (CRD) consist of 4 treatments, each treatment consisted of 5 replicates so that the number of samples are 20 observations. Obtained data were tested with Analyze of Variant followed by Duncan's Multiple Range Test. The results of the study at day 10 indicated the ovicidal effect wudani leaf extract to doses 0,24mL / 40 NaCl physiological and dose 0,48mL /40 physiological NaCl was higher than the dose 0,12mL / 40 Physiological NaCl (P 0.01), while the results at day 30 showed a significant result that the dose 0,48mL / 40mL physiological NaCl was higher than the dose 0,24mL / 40mL physiological NaCl, the dose 0,12mL / 40mL physiological NaCl and control (P <0.01)