Global myocardial strain after the Ozaki aortic valve replacement in low-gradient critical aortic stenosis and heart failure: a case report

The article presents the results of 1,5-year follow-up after surgery of critical aortic stenosis by the Ozaki technique in a patient with severe heart failure using data on global longitudinal strain

Quantum dot loaded immunomicelles for tumor imaging

<p>Abstract</p> <p>Background</p> <p>Optical imaging is a promising method for the detection of tumors in animals, with speed and minimal invasiveness. We have previously developed a lipid coated quantum dot system that doubles the fluorescence of PEG-grafted quantum dots at half the dose. Here, we describe a tumor-targeted near infrared imaging agent composed of cancer-specific monoclonal anti-nucleosome antibody 2C5, coupled to quantum dot (QD)-containing polymeric micelles, prepared from a polyethylene glycol/phosphatidylethanolamine (PEG-PE) conjugate. Its production is simple and involves no special equipment. Its imaging potential is great since the fluorescence intensity in the tumor is twofold that of non-targeted QD-loaded PEG-PE micelles at one hour after injection.</p> <p>Methods</p> <p>Para-nitrophenol-containing (5%) PEG-PE quantum dot micelles were produced by the thin layer method. Following hydration, 2C5 antibody was attached to the PEG-PE micelles and the QD-micelles were purified using dialysis. 4T1 breast tumors were inoculated subcutaneously in the flank of the animals. A lung pseudometastatic B16F10 melanoma model was developed using tail vein injection. The contrast agents were injected via the tail vein and mice were depilated, anesthetized and imaged on a Kodak Image Station. Images were taken at one, two, and four hours and analyzed using a methodology that produces normalized signal-to-noise data. This allowed for the comparison between different subjects and time points. For the pseudometastatic model, lungs were removed and imaged <it>ex vivo </it>at one and twenty four hours.</p> <p>Results</p> <p>The contrast agent signal intensity at the tumor was double that of the passively targeted QD-micelles with equally fast and sharply contrasted images. With the side views of the animals only tumor is visible, while in the dorsal view internal organs including liver and kidney are visible. <it>Ex vivo </it>results demonstrated that the agent detects melanoma nodes in a lung pseudometastatic model after a 24 hours wash-out period, while at one hour, only a uniform signal is detected.</p> <p>Conclusions</p> <p>The targeted agent produces ultrabright tumor images and double the fluorescence intensity, as rapidly and at the same low dose as the passively targeted agents. It represents a development that may potentially serve to enhance early detection for metastases.</p

Development of neonatal feeding devices: A historical excursus

The paper contains a review of available literature on neonatal feeding devices. It considers in detail the design and composition of materials to manufacture feeding bottles, pacifiers, and nipples

Пародонтопротекторное действие антидисбиотических средств при экспериментальном сахарном диабете 2 типа = Periodontitis-protective effecr of anti-dysbiotic means at experimental diabetes mellitus type 2

Stupak E. P., Skidan K. V., Skidan M. I., Dem'yanenko S. A. Пародонтопротекторное действие антидисбиотических средств при экспериментальном сахарном диабете 2 типа = Periodontitis-protective effecr of anti-dysbiotic means at experimental diabetes mellitus type 2. Journal of Education, Health and Sport. 2015;5(12):588-597. eISSN 2391-8306. DOIhttp://dx.doi.org/10.5281/zenodo.44264 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%2812%29%3A588-597 http://pbn.nauka.gov.pl/works/687233 Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011–2014http://journal.rsw.edu.pl/index.php/JHS/issue/archive   The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport (null) 2391-8306 7 © The Author (s) 2015; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 10.12.2015. Revised 15.12.2015. Accepted: 29.12.2015.   УДК 616.731-07.23.008   ПАРОДОНТОПРОТЕКТОРНОЕ ДЕЙСТВИЕ АНТИДИСБИОТИЧЕСКИХ СРЕДСТВ ПРИ ЭКСПЕРИМЕНТАЛЬНОМ САХАРНОМ ДИАБЕТЕ 2 ТИПА   PERIODONTITIS-PROTECTIVE EFFECR OF ANTI-DYSBIOTIC MEANS AT EXPERIMENTAL DIABETES MELLITUS TYPE 2   Е. П. Ступак1, К. В. Скидан2, М. И. Скидан2, С. А. Демьяненко3 E. P. Stupak1, K. V. Skidan2, M. I. Skidan2, S. A. Dem'yanenko3   1ВГУУ «Украинская медицинская стоматологическая академия», г. Полтава 2Харьковский национальный медицинский университет 3Крымский государственный медицинский университет им. С. И. Георгиевского, г. Симферополь   1HSEI «Ukrainian medical Stomatological Academy», Poltava 2Kharkiv national medical university, Kharkiv 3Crimean State Medical University named after S.I.Georgievskij, Simferopol       Abstract Aim: To determine the therapeutic and preventive effect of oral gels with anti-dysbiotic means (ADM) on periodont at type 2 diabetes mellitus (DMT2). Materials and Methods: Diabetes in rats was induced by an intra-muscular administration of protamine sulfate for 10 days. As ADM there were used lysozyme, quercetin, flour, mucosin and flour from vine leaves, which were applied in form of gels on the oral mucosa of rats for 2 weeks. In the gums' homogenates there were determined the activities of urease, lysozyme, elastase, catalase and the MDA content. The degree of dysbiosis by Levitsky was calculated as the ratio of the relative activities of urease and lysozyme. The antioxidant-prooxidant index API was calculated as the ratio of the catalase activity and the MDA content. Results: The urease activity, the degree of dysbiosis, the elastase activity and the MDA content were increased while the lysozyme activity and the index API were reduced in rats with diabetes type 2 in the periodont. Applied gels containing ADM showed therapeutic and preventive effects. Conclusion: At diabetes type 2 in the periodont of rats there were developing dysbiosis and inflammation. The application of oral gels with ADM provided a therapeutic and preventive treatment.   Keywords: diabetes type 2, periodont, dysbiosis, inflammation, anti-dysbiotic means, oral gels.     Резюме При экспериментальном сахарном диабете 2 типа в десне развивается дисбиоз и воспаление. Оральные гели с антидисбиотическими средствами (лизоцим, кверцетин, мукозин и мука из виноградных листьев) оказывают лечебно-профилактический эффект.   Ключевые слова: сахарный диабет 2 типа, пародонт, дисбиоз, воспаление, антидисбиотические средства, оральные гели

MAILLARD REACTIONS – AN IMPORTANT FACTOR OF THE SAFETY AND QUALITY OF INFANT FORMULA

The physicochemical properties of infant adapted milk formulae that affect their tolerability and effectiveness depend on the composition and quality of the raw ingredients, the production process, the storage conditions and the quality control of the finished products. The technology of manufacturing a powdered infant formula includes a variety of methodsfor processing componentsthat make up its composition, including raw milk. This processing is accompanied by a noticeable change in a number of physical, chemical and biological properties of the individual components of milk, their loss, the formation of fundamentally new chemical compounds. The most frequent reactions observed during the heat treatment of milk include the formation of bonds between reactive carbonyl groups of the sugar and the amino groups of amino acids, followed by the appearance of a large number of low- and high-molecular compounds, the so-called Maillard Reaction Products(MRP). The study of MRP in recent years hasincreasingly attracted the attention of medical practitioners because of the discovery of these compounds in infant formula and their potential danger to children’s health. This review provides evidence that powdered infant adapted formulae produced with an original technology based on whole goat milk with a native ratio of the main groups of milk proteins(whey – 20% and casein – 80%) have a minimum potential for unwanted effects associated with MRP

Prebiotic components of breast milk and the possibility of repeating their effects in infant formulas

It is fundamental truth that breast milk is the best food for a newborn, providing it with energy, high-quality nutrients, as well as a large variety of biologically active components necessary for adequate physical and psychomotor development. Acting either as part of complex biological structures or separately, the breast milk components, including personalized microbial and immune, regulate morphogenesis at the molecular and cellular level, determine the metabolism nature, guide the development of mucosal immunity in the child’s body. It is impossible to identify any component of breast milk as the main component that determines its functional activity and provides the benefits of natural feeding. The purpose of this review is to describe the role of prebiotics in breast milk and infant formula for the formation and maintenance of a healthy baby intestinal microbiota, which is a key element in the formation of normal digestion, a functionally sound immune system and metabolism

New complementary foods in the diet of breast-fed and bottle-fed infants

Objective: to evaluate the physical development and health status of babies receiving goat’s milk-based formula “Bibikasha”.Patients and methods. An open-label uncontrolled trial was conducted in 47 babies aged 5 to 6.5 months. Their weight and height changes, neuropsychological development, and the skin were assessed. The incidence of acute respiratory diseases, the manifestations of dyspepsia, and number of bowel movement a day were estimated; fecal macroscopy, microbiological examination, and complete blood count were carried out. The data were statistically processed using Statistica Advanced.Results. At complementary feeding, there was constipation and hard stools in approximately 20% the infants in the study group and fecal opportunistic bacteria in 63.8%. When eating Bibikasha, the number of infants with opportunistic pathogenic bacteria reduced by 3 times and hard stools and constipation disappeared completely. During their follow-up, none of the babies developed anemia, acute respiratory or gastrointestinal diseases; weight gain rates and psychomotor development were age-appropriate, indicating their harmonious development.Conclusion. Bibikasha used as a complementary food has a positive effect on a baby’s health and contributes to the prevention of nutrition-related diseases. Adding Bibikasha to the diet of infants with constipation normalizes their bowel functio