28 research outputs found
Activation of mouse lymphocytes by anti-immunoglobulin. IV. Stimulation with soluble heterologous anti-delta antibodies.
Activation of mouse lymphocytes by anti-immunoglobulin. II. A thymus-independent response by a mature subset of b lymphocytes.
VIKS rechnet sich. Moderne Innovationsprozesse bei der Einfuehrung eines Vertriebs-Informations- und Kommunikations-Systems Erfahrungen aus einem mittelstaendischen Anlagenbauunternehmen
SIGLEAvailable from TIB Hannover: F94B2139+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman
CBA/N Immune Defective Mice; Evidence for the Failure of a B Cell Subpopulation to be Expressed
Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
International audienceQuantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology
Interpretation of multifrequency transient EPR spectra of the P 700 + A0Q K − state in photosystem I complexes with a sequential correlated radical pair model: Wild type versus A0 mutants
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the approximately 21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community