Oxidative stress parameters changes in blood of rats treated with adriamycin and selenium

Meeting on Free Radicals and Oxidative Stress, 2003, Loannina, Greec

Effect of gamma irradiation on magnesium content in rat tissues

Studies in the field of radiation chemistry have shown that the greatest damage induced by ionizing radiation appears as a consequence of uncontrolled production of free radicals in the living organism, which have their origin in oxidative stress induced by ionizing radiation. Ionizing radiation also has an influence on the content of bio-essential elements in mammalian tissues. Analysis of the concentration of magnesium (Mg) seven days after whole body irradiation with a single dose of 4.2 Gy of gamma rays from a Co-60 source, showed a slight reduction in Mg content in the following analyzed tissues: front brain (cerebral hemispheres with underlying structures except pituitary gland and hypothalamus) and adrenal glands from irradiated animals as compared to the non-irradiated controls, this difference however being not statistically significant. No significant differences in the content of Mg were detected in the pituitary glands between the two groups of animals. In contrast, a significantly higher concentration of Mg seven days after irradiation was measured in the hind brain-containing cerebellum with lateral lobes and flocculi (p LT 0.005), hypothalamus (p LT 0.0001) and testes (p LT 0.05) of irradiated animals, when compared to the non-irradiated controls. This accumulation of Mg, in particular tissues of the Central Nervous System (CNS) and endocrine glands regulated by the pituitary gland and hypothalamus, may indicate the differential role Mg++ ions have in keeping the homeostasis of certain tissues, those of the brain, adrenal glands and testes after exposure to ionizing radiation

Influence of long-term selenium supplementation on antioxidant trace elements in the rat brain

Metal Ions in Biology and Medicine, 8th International Symposium on Metal Ions in Biology and Medicine, May 18-22, 2004, Hungarian Acad Sci, Budapest, Hungar

Influence of long-term selenium supplementation on antioxidant trace elements in the rat brain

Metal Ions in Biology and Medicine, 8th International Symposium on Metal Ions in Biology and Medicine, May 18-22, 2004, Hungarian Acad Sci, Budapest, Hungar

Selenium and Vitamin E Modulates Radiation-Induced Liver Toxicity in Pregnant and Nonpregnant Rat: Effects of Colemanite and Hematite Shielding

The levels of liver lipid peroxidation, glutathione peroxidase, reduced glutathione, and vitamins A and E were used to follow the level of oxidative damage caused by ionizing radiation in pregnant rats. The possible protective effects of selenium and vitamin E supplemented to rats housed in concrete-protected cages using hematite and colemanite were tested and compared to untreated controls. Ninety-six rats were randomly divided into four main equal groups namely control (A), normal concrete (B), concrete containing colemanite (C), and concrete containing hematite (D). Except group A, all groups exposed to 7 Gy radiation. The four main groups were divided into four subgroups each as follows: subgroups 1 (n = 6): nonpregnant control rats. Subgroups 2 (n = 6): selenium and vitamin E combination was intraperitoneally (i.p.) given to the nonpregnant rats for 20 days. Subgroups 3 (n = 6): pregnant control rats. Subgroups 4 (n = 6): selenium and vitamin E combination was i.p. given to the pregnant rats for concessive 20 days. Lactate dehydrogenate, alkaline phosphates, and lipid peroxidation values were higher in subgroups 1 and 3 than in no radiation group although glutathione peroxidase and vitamin E levels in liver were lower in radiation group than in no radiation group. Lactate dehydrogenate activity and lipid peroxidation levels were found to be decreased in subgroups 2 and 4 protected with concrete containing hematite and colemanite when compared to subgroup 1 and 3 with normal concrete. The radiation doses in rats housed by concrete without colemanite and hematite exposed radiation clearly showed liver degeneration. In conclusion, selenium and vitamin E supplementations and housing by concrete with colemanite was found to offer protection against gamma-irradiation-induced liver damage and oxidative stress in rats, probably by exerting a protective effect against liver necrosis via its free radical scavenging and membrane stabilizing. Protective effects of colemanite in the liver seem to be more important than in hematite