Psychotic aura symptoms in familial hemiplegic migraine type 2 (ATP1A2)

Abstract INTRODUCTION: Neuropsychological symptoms are rare in familial hemiplegic migraine (FHM). There are no reports of psychotic symptoms in FHM type 2 (ATP1A2). We examined a family with a FHM phenotype due to a M731T mutation in ATP1A2. A 10-year follow-up allowed us to observe complex auras, including psychotic symptoms in two siblings. CASE REPORT: Male, 48 years old, with an aura that included complex illusions with a feeling of time travelling, coincident with other aura features. The aura was regarded as mystical by the patient. Female, 38 years old, with a complex migraine aura, during which she believed she had the ability to time travel and was being followed by lobbyists who wanted to steal this ability from her. DISCUSSION: FHM type 2 must be included in the list of differential diagnoses of acute psychosis in patients with a previous history of migraine aura

À descoberta de sofware para explorar a programação linear no ensino secundário

A Programação Linear, PL, tem como objectivo a resolução de problemas de optimização com restrições em que todas as funções envolvidas são lineares. Diversos problemas da vida real podem ser formulados como problemas de PL como, por exemplo, os problemas de planeamento e de transportes. A Programação Linear é um dos temas obrigatórios de algumas disciplinas de Matemática do Ensino Secundário, sendo importante que se trabalhem com os alunos problemas que traduzam situações reais, ou suas adaptações. Como estes problemas nem sempre são fáceis e rápidos de resolver, a utilização de ferramentas tecnológicas na sua resolução reveste-se de enorme importância. Existem diversos programas de computador como o Solver do Microsoft Office Excel, o WinQSB, o Programación Lineal e o Winplot, de fácil utilização pelos alunos, que possibilitam a exploração gráfica, no caso bidimensional, ou analítica dos problemas de PL. O uso deste software permite que os alunos resolvam uma maior diversidade de problemas e se centrem mais na análise e interpretação de resultados. Neste artigo, ilustra-se, resumidamente, a forma de resolver problemas de PL com o software mencionado e discute-se as suas potencialidades e limitações

Linear programming knowledge in first stage higher education courses

In this work we will describe an experimental study with approximately fifty Portuguese students of the 2nd year of the BSc Degree of Biomedical Engineering as well as Chemical and Biological Engineering Higher Education Courses. The aims of this study are to identify which knowledge of Linear Programming (LP) taught in High School the students acquired and still remember, which tools they used in that stage of education when learning the theme of LP and their opinion about the significance of LP in daily life problem resolution. We also propose to investigate if the students are able to use WinQSB software to solve LP problems. In addition, we intend to identify not only the versatility of this software but also the opinion of the students about the resolution of LP problems with WinQSB, as well as their main difficulties during this experience

Boro no crescimento de mudas de erva-mate em solução nutritiva.

A maioria das soluções nutritivas indicam a dose de 0,5 mg L-1 de boro (B) como ideal, mas para erva-mate não há informações sobre sua necessidade em B. Avaliou-se o crescimento de mudas clonais de erva-mate em solução nutritiva, submetidas a doses de: 0,0; 1,0; 2,5 e 5,0 mg L-1 de B. O experimento foi conduzido em Viçosa-MG e após 80 dias, através de análise de regressão, avaliou-se características de crescimento das mudas onde obteve-se que doses de B próximas de 2,90 mg L-1 favoreceram o melhor crescimento em altura, diâmetro do colo e comprimento do sistema radicular. Já para maximizar a produção de matéria seca, volume do sistema radicular, área foliar e espessura foliar a erva-mate necessita de doses próximas a 3,00 mg L-1 de B. Conclui-se que mudas de erva-mate, conduzidas em solução nutritiva, necessitam de 2,5 a 3,1 mg L-1 de B para um bom crescimento

BDNF and CGRP interaction: implications in migraine susceptibility

Abstract OBJECTIVES: Migraine pathophysiology involves several pathways. Our aims were to explore a possible role of the brain-derived neurotrophic factor gene (BDNF) in migraine susceptibility; to study, for the first time, the calcitonin gene-related peptide gene (CGRP); and a possible interaction between the two. METHODS: Using a case-control approach, four tagging single nucleotide polymorphisms (SNPs) (rs7124442, rs6265, rs11030107, and rs2049046) of BDNF and one tagging SNP-rs1553005-of CGRP were analyzed in 188 cases and 287 controls. A multivariable logistic regression was performed, adjusting for gender. Allelic and haplotypic frequencies were estimated. Interaction was assessed by a stepwise multivariable-logistic regression and confirmed by a multifactor dimensionality reduction analysis. RESULTS: No significant main effects were found; however, a significant interaction was found between BDNF and CGRP, showing an increased risk for the AT-genotype of rs2049046 and the GC-genotype of rs1553005 (odds ratio=1.88, 95% confidence interval: 1.20-2.93) for migraineurs. CONCLUSION: Our data support the hypothesis of an interaction between BDNF and CGRP in migraine susceptibility that should be further explored

How gamma radiation affects antimicrobial potential of medicinal plants: Mentha x piperita L. as a case study

Medicinal plants are being used by the food and pharmaceutic industries to extract relevant compounds. To be used in these industries as raw materials, it is required the non-presence of microorganisms. Therefore, it is essential that they undergo a decontamination process before entering processing lines, and these methods should not compromise their bioactive properties. In this context a non-thermal technology, gamma irradiation, was used as a decontamination procedure. For this purpose, samples of Mentha piperita L. (peppermint) was irradiated using a 60Co experimental chamber, at the doses of 1, 5, and 10 kGy. The hydroethanolic extracts were then further evaluated through the virucidal efficacy assay, using two enteric viruses – human adenovirus type-5 (HAdV-5) and murine norovirus type-1 (MNV-1, as a human norovirus surrogate). The antibacterial activity was evaluated against a set of Gram-positive and Gram-negative bacteria. For the virucidal activity, data suggested that the irradiation treatment of all species can preserve the natural properties of the plant against enteric viral pathogens. The hydroethanolic extracts of peppermint reveal a low antibacterial potential for all the tested bacteria. However, antibacterial activity of gamma irradiated samples did not affect this bioactivity for none of the microbial agents tested. Validation of antimicrobial potential of medicinal plants, after gamma irradiation processing, are scarce in the literature. This study allowed defining the influence of this treatment on the bioactive properties of peppermint.Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013), C2TN (UID/Multi/04349/2013), grant to A. Fernandes (SFRH/BPD/114753/2016) and L. Barros contract; European Structural and Investment Funds (ESIF) (Regional Operational Program Norte 2020, Project ValorNatural®); Rural Development Program (Project MicoCoating, PDR2020-101-031472); International Atomic Energy Agency (IAEA) Coordinated Research Project D61024 DEXAFI.info:eu-repo/semantics/publishedVersio

Evidence of syntaxin 1A involvement in migraine susceptibility: a Portuguese study.

Abstract OBJECTIVE: To confirm syntaxin 1A as a risk factor for migraine, given that syntaxin 1A interacts with several factors in migraine pathophysiology. DESIGN: Case-control approach. SETTING: An outpatient clinic. PARTICIPANTS: In a sample of 188 migraineurs (111 without aura and 77 with aura) and 287 migraine-free controls, 3 tagging SNPs of STX1A (rs3793243, rs941298, and rs6951030) were analyzed. A backward stepwise multiple logistic regression was performed. Allelic and haplotypic frequencies were compared between cases and controls. RESULTS: We found that rs941298 and rs6951030 were risk factors for migraines. In particular, the TT genotype of rs941298 is associated with an increased risk of both migraine in general and migraine without aura; the GG and GT genotypes for rs6951030 are also associated with migraine, while the GT genotype of rs6951030 was found to be significant in the migraine without aura group. The single-nucleotide polymorphism rs3793243 did not show any significant association. In the haplotype-based analysis, we found an underrepresentation of the T-C-T haplotype (rs3793243-rs941298-rs6951030) in the global sample and in migraine without aura group. We found an enrichment of the G allele of rs6951030 for female migraineurs only. CONCLUSIONS: We confirmed the involvement of syntaxin 1A in migraine susceptibility regarding rs941298. In addition, we found rs6951030 to also be associated in Portuguese migraine patients. Sex differences should be further explored to disentangle a possible sex susceptibility in syntaxin 1

A role for endothelin receptor type A in migraine without aura susceptibility? A study in Portuguese patients

Abstract BACKGROUND AND PURPOSE: Migraine is a common neurological disabling disorder, and anomalies of vascular function have been implied in its pathophysiology. Several findings point to a possible role of the endothelin receptor type A (EDNRA) in migraine. We aim to assess the involvement of endothelin receptor type A (EDNRA) in migraine susceptibility in a sample of Portuguese migraineurs. METHODS: Three tagging SNPs (rs702757, rs5333 and rs5335) were analysed in 188 cases - 111 without aura (MO) and 77 with aura (MA) - and 287 controls. A multivariable logistic regression was performed, including the three SNPs, adjusted for gender. Allelic and haplotypic frequencies were compared between cases and controls. Significant or promising results were confirmed by a multifactor dimensionality reduction analysis (MDR). RESULTS: We found a nominal association for the rs702757 T-allele [odds ratio (OR) = 1.44, 95% confidence intervals (CI): 1.05-1.99] and for the TT-genotype (OR = 2.34, 95% CI: 1.12-4.90) for MO, that do not remain significant after multiple test correction. A trend towards an increased risk for MA regarding the C-allele of rs5333 was also found. However, an additional MDR analysis was performed, and highly significant results were found for the two SNPs. The T-C-G haplotype (rs702757-rs5333-rs5335) was found to be significantly overrepresented in the MO subgroup, even after permutation was performed. CONCLUSIONS: Our results show additional findings for a role of EDNRA as a susceptibility factor for MO, although we cannot exclude the involvement of this gene in MA susceptibility in our population. Our study also emphasizes the need for replication of association findings in different populations