Diagnostic Test Equivalent Hemoglobin Reticulocyte in Iron Deficiency Anemia

BACKGROUND: Diagnosing iron deficiency anemia (IDA) is easy, but also can be complicated in condition with inflammation. A new modality for diagnostic which isn’t influenced with inflammation is needed. The aim of this study is to find the cut-off point and evaluate the accuracy of reticulocyte hemoglobin equivalent (Ret-He) to diagnose IDA using ferritin as the gold standard.METHODS: This study was an observational study with cross-sectional analytical design continued with the diagnostic test conducted in anemic individuals with age 18 years old or above.RESULTS: Eighty-seven patients (41 men and 46 women) were included in this study with mean of hemoglobin 7.42 g/dL, serum iron 42.71 mg/dL, total iron-binding capacity (TIBC) 242.82 mg/dL, ferritin 799 ug/L and Ret-He 23.63 pg. Ret-He with cut-off value 25 pg showed a sensitivity 97.2% (95% CI 83.79-99.85%), specificity 66.67% (95% CI 51.97-78.85%), positive predictive value 67.30% (95% CI 52.77-79.28%) and negative predictive value 97.14% (95% CI 83.38-99.85%).CONCLUSION: Ret-He showed the best sensitivity for detection of IDA and was suggested as the screening test for IDA.KEYWORDS: IDA, Ret-He, diagnostic tes

Concanavalin A Enhanced Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells

Objective Dental pulp stem cells (DPSCs) can be used as a component in the formation of regenerative dentine during direct pulp capping therapy. Concanavalin A (ConA) is a type of lectin with a molecular weight of 26 kDa derived from the Canavalia ensiformis plant. Lectins possess strong proliferation and differentiation abilities in various animal cells including lymphocytes, osteoblasts, and chondrocytes. The aim of study was to determine the effect of ConA on the proliferation and osteogenic differentiation of DPSCs in vitro. Materials and Methods In this in vitro study, DPSCs were isolated from third molars before ConA induction was performed at concentrations of 5 and 10 μg/mL. The proliferation assay was determined by 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Osteogenic differentiation was determined by means of mineralization. Statistical Analysis Data were analyzed using analysis of variance and a Student’s t-test. The p-value was set at 0.05. Results The addition of 5 and 10 µg/mL of ConA to DPSCs can significantly increase the proliferation and osteogenic differentiation of DPSCs (p ≤0.05). Conclusion ConA can increase the proliferation and osteogenic differentiation of DPSCs

Mutant P53 Expression Of Oral Transformed Epithelium Cell In Rats Injected By Benzo[A]Pyrene

Benzopyrene is a polycyclic aromatic hydrocarbon (PAH) compound that can cause transformation of normal cells into malignant. Benzopyrene enters the body and is converted to benzopyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), making it easier to bind covalently with DNA, causing DNA mutation. Tumor suppressor gene p53, plays an important role in cell cycle arrest at checkpoint phase and inducing apoptosis of cells that have DNA damage. DNA mutation in tumor suppressor genes make cells immortal which results in cell transformation and develops into malignancies. This research is a True Experimental Laboratories with Posttest only control group design. 25 RattusNovergicus were randomly divided into five groups including the control group (without benzopyrene injection) and 4 treatment groups injected with benzopyrene each for 4 weeks (P1), 6 weeks (P2), 8 weeks (P3) and 10 weeks (P4). The transformation cells formed was obtained by HE staining. Mutant p53 expression was obtained by Immunohistochemistry process. Histopathological examination was performed using a 400x magnification light microscope with 5 different visual fields. Therefore, the expression of mutant p53 was calculated and analyzed statistically with the One Way Anova Test. Results of this study showed that there was signifficance difference in mutant p53 expression of oral transformed epithelium cell injected by benzopyrene (p=0.000) and highest expression was at 10th week. So, it can be concluded that the mutant P53 expression of oral transformed epithelium cells in rats was increased after injected by benzopyren