TOTAL SIALIC ACID LEVELS IN HUMAN FOLLICULAR FLUID: A PRELIMINARY STUDY

International-Federation-of-Fertility-Societies 21st World Congress on Fertility and Sterility / 69th Annual Meeting of the American-Society-for-Reproductive-Medicine -- OCT 12-17, 2013 -- Boston, MAWOS: 000342554501238…Int Federat Fertil Soc, Amer Soc Reprod Me

Psödoeksfoliasyon Sendromu ve Psödoeksfoliasyon Glokomda Seruloplazmin ve Katalaz Aktiviteleri

Objective: Pseudoexfoliation syndrome (PEX) is an age-related systemic disorder primarily with ocular manifestations. PEX is considered as the most common identifiable cause of glaucoma (pseudoexfoliation glaucoma, PEG) and loss of vision. Oxidative stress was suggested to be involved in the pathogenesis of PEX and PEG. Catalase is an antioxidant enzyme which catalyzes decomposition of hydrogen peroxide to water and oxygen. Ceruloplasmin was reported to have antioxidant functions, as well as pro-oxidant activity. This study has aimed to determine whether ceruloplasmin and catalase activities have a role in the development of PEX and/or PEG. Material and Methods: Ceruloplasmin and catalase activities were determined in serum samples of 32 cases of PEX, 30 cases of PEG and 32 control subjects using spectrophotometric methods. Results: Ceruloplasmin activities of PEX patients (32.94±16.36 U/L) and PEG patients (29.98±13.15 U/L) were similar and slightly lower than those of controls (35.22±21.32 U/L). However, the differences were not statistically significant. Catalase activites were found to be 61.96±33.95 U/L and 60.44±50.84 U/L in PEX and PEG patients, respectively. Controls had slightly, but insignificantly, higher catalase activities (64.98±58.14 U/L). Conclusion: Neither catalase nor ceruloplasmin activities are significantly related with PEX or PEG in the studied Turkish population. As the mean age of populations keeps increasing, PEX may become more common in the future. Therefore, further studies are needed to decipher the pathophysiology of this important disorder.Amaç: Psödoeksfoliasyon sendromu (PES) öncelikle oküler bulguları olan, yaşa bağlı sistemik bir hastalıktır. PES, glokomun (psödoeksfoliasyon glokom, PEG) ve görme kaybının en yaygın tanımlanabilir nedeni olarak kabul edilir. Oksidatif stresin PES ve PEG patogenezinde rolü olduğu öne sürülmüştür. Katalaz, hidrojen peroksitin su ve oksijene ayrışma reaksiyonunu katalize eden antioksidan bir enzimdir. Seruloplazminin antioksidan fonksiyonları yanında, pro-oksidan aktiviteye de sahip olduğu bildirilmiştir. Bu çalışmada seruloplazmin ve katalaz aktivitelerinin PEX ve/veya PEG gelişiminde rolü olup olmadığını belirlemek amaçlanmıştır. Gereç ve Yöntemler: Seruloplazmin ve katalaz aktiviteleri spektrofotometrik yöntemlerle 32 PES'li olgu, 30 PEG'li olgu ve 32 kontrol bireyin serum örneklerinde belirlenmiştir. Bulgular: PES hastalarının (32,94±16,36 U/L) ve PEG hastalarının (29,98±13,15 U/L) seruloplazmin aktiviteleri birbirine çok benzer seviyede ve kontrollerinkinden (35,22±21,32 U/L) biraz düşüktü. Ancak aradaki fark istatistiki olarak anlamlı değildi. Katalaz aktiviteleri PES ve PEG hastalarında sırasıyla 61,96±33,95 U/L ve 60,44±50,84 U/L olarak bulundu. Kontroller ise, biraz daha yüksek katalaz aktivitesine sahipti (64,98±58,14 U/L). Sonuç: Çalışılan Türk popülasyonunda katalaz ve seruloplazmin aktiviteleri PES veya PEG ile istatistiki olarak anlamlı biçimde ilişkili değildir. Popülasyonların ortalama yaşı artmaya devam ettiğinden, PEX gelecekte daha yaygın hale gelebilir. Bu nedenle, bu önemli hastalığın patofizyolojisini deşifre etmek için daha ileri çalışmalar gerekmektedir

MEASUREMENT OF THE SERUM AND FOLLICULAR FLUID TOTAL OXIDANT STATUS, TOTAL ANTIOXIDANT STATUS, OXIDATIVE STRESS INDEX IN PATIENT WITH HIGH, NORMAL OR POOR RESPONSE

International-Federation-of-Fertility-Societies 21st World Congress on Fertility and Sterility / 69th Annual Meeting of the American-Society-for-Reproductive-Medicine -- OCT 12-17, 2013 -- Boston, MAWOS: 000342554501739…Int Federat Fertil Soc, Amer Soc Reprod Me

The effects of chronic periodontitis and rheumatoid arthritis on serum and gingival crevicular fluid total antioxidant/oxidant status and oxidative stress index

Background: Chronic periodontitis (CP) and rheumatoid arthritis (RA) appear to share many pathologic features. Oxygen metabolism has an important role in the pathogenesis of both CP and RA. The aim of this study is to evaluate the relationship between these two chronic inflammatory diseases with regard to antioxidant and oxidant status. Methods: A total of 80 participants were divided into four groups of 20 each: group RA–CP (patients with RA and CP), group RA (periodontally healthy patients with RA), group CP (systemically healthy patients with CP), and group C (periodontally and systemically healthy volunteers) were included in the study. After assessment of periodontal measurements, gingival crevicular fluid (GCF) samples were taken at one incisor, premolar, and molar tooth and stored with serum samples at -80 C for the antioxidant/oxidant assay. Results: Although all clinical measurements in groups RA–CP and CP were statistically higher compared to those of C and RA groups (P 0.05). GCF total oxidant status (TOS) values of CP and RA–CP groups were higher than those of the RA group (P 0.05). Conclusions: Local OSI values in groups with patients with CP were higher, whereas systemic OSI values showed no difference among the groups. The presence of RA seems not to affect local and systemic OSI values in patients with C