Retroperitoneal Laparoscopy in Dogs: Access Technique, Working Space, and Surgical Anatomy

Objective: To develop and describe a laparoscopic retroperitoneal access technique, investigate working space establishment, and describe the surgical anatomy in the retroperitoneal space as an initial step for clinical application of retroperitoneal laparoscopy in dogs. Study Design: Cadaveric and experimental study. Animals: Cadaveric (n58) and healthy (n56) adult dogs. Methods: The retroperitoneal access technique was developed in 3 cadavers based on the human technique and transperitoneal observation. Its application and working space establishment with carbon dioxide (CO2) insufflation alone was evaluated in 5 cadavers by observing with a transperitoneal telescope and in 6 live dogs by repeated computed tomography (CT) scans at pressure of 0, 5, 10, and 15 mmHg. Recordings of retroperitoneoscopy as well as working space volume and linear dimensions measured on CT images were analyzed. Results: Retroperitoneal access and working space establishment with CO2 insufflation alone were successfully performed in all 6 live dogs. The only complication observed was in 1 dog that developed subclinical pneumomediastinum. As pressure increased, working space was established from the ipsilateral to the contralateral side, and peritoneal tearing eventually developed. Working space volume increased significantly from 5 mmHg and linear dimensions increased significantly from 0 to 10 mmHg. With pneumo-retroperitoneum above 5 mmHg, retroperitoneal organs, including kidneys and adrenal glands, were easily visualized. Conclusion: The retroperitoneal access technique and working space establishment with CO2 insufflation starting with 5 mmHg and increasing to 10 mmHg provided adequate working space and visualization of retroperitoneal organs, which may allow direct access for retroperitoneal laparoscopy in dogs.OAIID:RECH_ACHV_DSTSH_NO:T201622608RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A000300CITE_RATE:1.295FILENAME:정준모.pdfDEPT_NM:수의학과EMAIL:[email protected]_YN:YFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/c102ca68-4a4d-4030-b323-ee99c3af083f/linkCONFIRM:

Identification of key regulators during the establishment of mouse intestinal organoid from embryonic stem cells

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Comparative Genome-wide Analysis of Intestinal Organoids Derived from Mouse GI Tract Adult Stem cells and Embryonic Stem Cells

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Chromatin structural changes during intestinal organoid generation from mouse embryonic stem cells

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Single-cell transcriptome sequencing of plant leaf expressing anti-HER2 VHH–FcK cancer therapeutic protein

Abstract The transgenic plant is a promising strategy for the production of highly valuable biotherapeutic proteins such as recombinant vaccines and antibodies. To achieve an efficient level of protein production, codon sequences and expression cassette elements need to be optimized. However, the systematical expression of recombinant proteins in plant biomass can generally be controlled for the production of therapeutic proteins after the generation of transgenic plants. Without understanding the transgene expression patterns in plant tissue, it is difficult to enhance further production levels. In this study, single-cell RNA-sequencing (scRNA-seq) analysis of transgenic tobacco (Nicotiana tabacum) leaf, expressing an immunotherapeutic llama antibody against breast cancer, anti-HER2 VHH–Fc, was conducted to obtain data on the expression pattern of tissue-specific cells. These high-quality scRNA-seq data enabled the identification of gene expression patterns by cell types, which can be applied to select the best cell types or tissues for the high production of these recombinant antibodies. These data provide a foundation to elucidate the mechanisms that regulate the biosynthesis of recombinant proteins in N. tabacum

Extractive Desulfurization Using Fe-Containing Ionic Liquids

Fe^III-containing ionic liquids (ILs), prepared from the reaction of anhydrous FeCl₃ and imidazolium chloride ([imidazolium]Cl), were used as effective extractants for the desulfurization of a model oil containing dibenzothiophene (DBT). The amount of DBT extracted increased with an increasing molar ratio of FeCl₃/[imidazolium]Cl. The ability of the ILs to extract DBT seems to be attributed to the combined effects of Lewis acidity and fluidity of ILs