Pengaruh Kualitas Produk, Harga, Dan Saluran Distribusi Terhadap Loyalitas Pelanggan Majalah Swa Melalui Variabel Kepuasan Pelanggan (Studi Kasus Pada Pelanggan Majalah Swa Di DKI Jakarta)

Customer loyalty is a goal that has to be achieved by a company. To be able to get loyal customers, SWA magazine needs to pay attention on the factors that influence customers\u27 loyalty. Moreover, business and economy themed magazines are mushrooming, leading to the opportunity for the readers to move from one magazine to another.This research aimed to ascertain the effect of product quality, price, and distribution channel on customer loyalty of SWA magazine in Jakarta through customer satisfaction variable both simultaneously and partially. The hypothesis was there was an effect of product quality, price, and distribution channel on customer loyalty of SWA magazine in Jakarta through customer satisfaction variable both simultaneously and partially. The type of this research was explanatory research with 97 respondents with multi stage sampling technique through questionnaire and interview. The data was analyzed using linear regression method with the assistance of SPSS 16.0.The result of this research showed that product quality, price and distribution channel variables had significant and positive effect partially on customer satisfaction. Product quality variable did not have partially significant effect on customer loyalty. Price and distribution channel variables had partially significant and positive effect on customer loyalty. Product quality and price variables had simultaneously positive and significant effect on customer satisfaction while distribution channel had simultaneously negative effect on customer satisfaction. Simultaneously, product quality, price, and distribution channel variables had positive effect and not significant effect on customer loyalty. Partially, customer satisfaction had positive and significant effect on customer loyalty.Based on the result of this research, a conclusion was drawn that customers\u27 perception on product quality, price, and distribution channel was good. Customers\u27 satisfaction and loyalty of SWA magazine were also good. The company was suggested to improving the product quality, adjusting the price and boosting the distribution channel of SWA magazine in accordance with customers\u27 needs and expectation, so that, customers can feel the satisfaction and decided to be loyal customers

Summary of clinical data for the affected patients in the study family.

<p>Summary of clinical data for the affected patients in the study family.</p

Ablation of endogenous BRD4 expression in HLE-B3 cells using shRNA.

<p>HLE-B3 cells were not transfected (wild-type), mock-transfected (shNT), or transfected with shRNA targeting <i>BRD4</i> (shBRD4) for 48 h, and were then subjected to RT-PCR (A) and western blotting (B) to detect BRD4 mRNA and protein levels. β-Actin and GAPDH served as loading controls. ShBRD4 significantly ablated endogenous BRD4 at both the mRNA and protein levels. Decreases in p53, p62, LC3BI, and CX43 protein levels, and an increase in LC3BII were detected after BRD4 knockdown. Protein levels were analyzed by western blotting. Western blots from three independent experiments were quantified and normalized against β-actin. The quantities were determined using ImageJ (National Institutes of Health) and all data were normalized to β-actin. ***<i>p</i> < 0.001 vs. wild type.</p

Clinical findings of the proband and preoperative eyes of the father.

<p>(A) In the upper panel, both of the father’s eyes showed white congenital cataracts before preoperative slit-lamp images were obtained, and the lower panel shows slit-lamp images for both of the proband’s eyes, showing postoperative bilateral phacoemulsification with posterior chamber lens implantations. (B) Brachydactyly on both hands was observed and hand X-rays revealed short 3rd and 4th left metacarpals and a short right 3rd metacarpal. (C) Macrocephaly without definite brain anomalies on 1.5-tesla magnetic resonance imaging. (D) Foot radiographs showing flat feet and both accessory navicular bones.</p

Summary of five candidate variants with a consistent inheritance pattern detected by whole exome sequencing analyses.

<p>Summary of five candidate variants with a consistent inheritance pattern detected by whole exome sequencing analyses.</p

Identification of a Novel Mutation in <i>BRD4</i> that Causes Autosomal Dominant Syndromic Congenital Cataracts Associated with Other Neuro-Skeletal Anomalies

<div><p>Congenital cataracts can occur as a non-syndromic isolated ocular disease or as a part of genetic syndromes accompanied by a multi-systemic disease. Approximately 50% of all congenital cataract cases have a heterogeneous genetic basis. Here, we describe three generations of a family with an autosomal dominant inheritance pattern and common complex phenotypes, including bilateral congenital cataracts, short stature, macrocephaly, and minor skeletal anomalies. We did not find any chromosomal aberrations or gene copy number abnormalities using conventional genetic tests; accordingly, we conducted whole-exome sequencing (WES) to identify disease-causing genetic alterations in this family. Based on family WES data, we identified a novel <i>BRD4</i> missense mutation as a candidate causal variant and performed cell-based experiments by ablation of endogenous <i>BRD4</i> expression in human lens epithelial cells. The protein expression levels of connexin 43, p62, LC3BII, and p53 differed significantly between control cells and cells in which endogenous <i>BRD4</i> expression was inhibited. We inferred that a <i>BRD4</i> missense mutation was the likely disease-causing mutation in this family. Our findings may improve the molecular diagnosis of congenital cataracts and support the use of WES to clarify the genetic basis of complex diseases.</p></div

BRD4 protein expression profile in mouse tissues obtained from two male mice.

<p>Protein levels of BRD4 and β-actin were analyzed by western blotting and protein quantities were determined using ImageJ (National Institutes of Health, Bethesda, MD, USA) by densitometry. Relative ratios of the Brd4/β-actin proteins are shown.</p

Pedigree and Sanger sequencing of the family with congenital cataracts and amino acid sequence conservation.

<p>(A) Pedigree of the family. The three generations of the family comprised four affected members (I-2, II-2, III-1, and III-2) and six unaffected members. The black arrow indicates the proband (III-2). Darkened symbols represent affected members. Stars indicate sampled subjects. Genotypes at the <i>BRD4</i> mutation site are indicated below each examined family member. (B) Sequencing chromatograms, vertical arrows indicate the mutation site. Sanger sequencing confirmed the <i>BRD4</i> heterozygous mutation (c.910C>T, p.His304Tyr) in all patients (II-3, III-2, and III-1) and the wild-type genotype in the unaffected family members (II-5 and III-3). (C) Conservation analysis of the amino acid sequence; the mutation site was highly conserved across vertebrate species.</p

Additional file 2: Figure S1. of Exploring evidence of positive selection reveals genetic basis of meat quality traits in Berkshire pigs through whole genome sequencing

The distributions of novel SNV and known SNP in each chromosome. Figure S2. A statistical enrichment test (Fisher’s exact test) for detecting enriched non-synonymous SNP site on targeted genes. Figure S3. A statistical enrichment test (Fisher’s exact test) for detecting enriched Indel site on targeted genes. Figure S4. The overall reads mapping rate of assembled contigs for each breed and reference genome by aligning the total sequence reads of each Berkshire sample. (DOCX 3433 kb

Additional file 1: of The genome landscape of indigenous African cattle

Supplemental data including Note S1, Tables S1-S7 and Figures S1-S12. (DOCX 14235 kb