553 research outputs found
Repetitive Segmental Structure of the Transducin β Subunit: Homology with the CDC4 Gene and Identification of Related mRNAs
Retinal transducin, a guanine nucleotide regulatory protein (referred to as a G protein) that activates a cGMP phosphodiesterase in photoreceptor cells, is comprised of three subunits. We have identified and analyzed cDNA clones of the bovine transducin β subunit that may be highly conserved or identical to that in other G proteins. From the cDNA nucleotide sequence of the entire coding region, the primary structure of a 340-amino acid protein was deduced. The encoded β subunit has a Mr of 37,375 and is comprised of repetitive homologous segments arranged in tandem. Furthermore, significant homology in primary structure and segmental sequence exists between the β subunit and the yeast CDC4 gene product. The Mr 37,375 β subunit polypeptide is encoded by a 2.9-kilobase (kb) mRNA. However, there exists in retina other β-related mRNAs that are divergent from the 2.9-kb mRNA on the basis of oligonucleotide and primer-extended probe hybridizations. All mammalian tissues and clonal cell lines that have been examined contain at least two β-related mRNAs, usually 1.8 and 2.9 kb in length. These results suggest that the mRNAs are the processed products of a small number of closely related genes or of a single highly complex β gene
CASTER: a scintillator-based black hole finder probe
The primary scientific mission of the Black Hole Finder Probe (BHFP), part of the NASA Beyond Einstein program, is to survey the local Universe for black holes over a wide range of mass and accretion rate. One approach to such a survey is a hard X-ray coded-aperture imaging mission operating in the 10-600 keV energy band, a spectral range that is considered to be especially useful in the detection of black hole sources. The development of new inorganic scintillator materials provides improved performance (for example, with regards to energy resolution and timing) that is well suited to the BHFP science requirements. Detection planes formed with these materials coupled with a new generation of readout devices represent a major advancement in the performance capabilities of scintillator-based gamma cameras. Here, we discuss the Coded Aperture Survey Telescope for Energetic Radiation (CASTER), a concept that represents a BHFP based on the use of the latest scintillator technology
CASTER - a concept for a Black Hole Finder Probe based on the use of new scintillator technologies
The primary scientific mission of the Black Hole Finder Probe (BHFP), part of
the NASA Beyond Einstein program, is to survey the local Universe for black
holes over a wide range of mass and accretion rate. One approach to such a
survey is a hard X-ray coded-aperture imaging mission operating in the 10--600
keV energy band, a spectral range that is considered to be especially useful in
the detection of black hole sources. The development of new inorganic
scintillator materials provides improved performance (for example, with regards
to energy resolution and timing) that is well suited to the BHFP science
requirements. Detection planes formed with these materials coupled with a new
generation of readout devices represent a major advancement in the performance
capabilities of scintillator-based gamma cameras. Here, we discuss the Coded
Aperture Survey Telescope for Energetic Radiation (CASTER), a concept that
represents a BHFP based on the use of the latest scintillator technology.Comment: 12 pages; conference paper presented at the SPIE conference "UV,
X-Ray, and Gamma-Ray Space Instrumentation for Astronomy XIV." To be
published in SPIE Conference Proceedings, vol. 589
The White Dwarf Cooling Sequence of NGC6397
We present the results of a deep Hubble Space Telescope (HST) exposure of the
nearby globular cluster NGC6397, focussing attention on the cluster's white
dwarf cooling sequence. This sequence is shown to extend over 5 magnitudes in
depth, with an apparent cutoff at magnitude F814W=27.6. We demonstrate, using
both artificial star tests and the detectability of background galaxies at
fainter magnitudes, that the cutoff is real and represents the truncation of
the white dwarf luminosity function in this cluster. We perform a detailed
comparison between cooling models and the observed distribution of white dwarfs
in colour and magnitude, taking into account uncertainties in distance,
extinction, white dwarf mass, progenitor lifetimes, binarity and cooling model
uncertainties. After marginalising over these variables, we obtain values for
the cluster distance modulus and age of \mu_0 = 12.02 \pm 0.06 and T_c = 11.47
\pm 0.47Gyr (95% confidence limits). Our inferred distance and white dwarf
initial-final mass relations are in good agreement with other independent
determinations, and the cluster age is consistent with, but more precise than,
prior determinations made using the main sequence turnoff method. In
particular, within the context of the currently accepted \Lambda CDM
cosmological model, this age places the formation of NGC6397 at a redshift z=3,
at a time when the cosmological star formation rate was approaching its peak.Comment: 56 pages, 30 figure
The Space Motion of the Globular Cluster NGC 6397
As a by-product of high-precision, ultra-deep stellar photometry in the
Galactic globular cluster NGC 6397 with the Hubble Space Telescope, we are able
to measure a large population of background galaxies whose images are nearly
point-like. These provide an extragalactic reference frame of unprecedented
accuracy, relative to which we measure the most accurate absolute proper motion
ever determined for a globular cluster. We find mu_alpha = 3.56 +/- 0.04 mas/yr
and mu_delta = -17.34 +/- 0.04 mas/yr. We note that the formal statistical
errors quoted for the proper motion of NGC 6397 do not include possible
unavoidable sources of systematic errors, such as cluster rotation. These are
very unlikely to exceed a few percent. We use this new proper motion to
calculate NGC 6397's UVW space velocity and its orbit around the Milky Way, and
find that the cluster has made frequent passages through the Galactic disk.Comment: 5 pages including 3 figures, accepted for publication in the
Astrophysical Journal Letters. Very minor changes in V2. typos fixe
Molecular Architecture and Functional Model of the Complete Yeast ESCRT-I Heterotetramer
SummaryThe endosomal sorting complex required for transport-I (ESCRT-I) complex, which is conserved from yeast to humans, directs the lysosomal degradation of ubiquitinated transmembrane proteins and the budding of the HIV virus. Yeast ESCRT-I contains four subunits, Vps23, Vps28, Vps37, and Mvb12. The crystal structure of the heterotetrameric ESCRT-I complex reveals a highly asymmetric complex of 1:1:1:1 subunit stoichiometry. The core complex is nearly 18 nm long and consists of a headpiece attached to a 13 nm stalk. The stalk is important for cargo sorting by ESCRT-I and is proposed to serve as a spacer regulating the correct disposition of cargo and other ESCRT components. Hydrodynamic constraints and crystallographic structures were used to generate a model of intact ESCRT-I in solution. The results show how ESCRT-I uses a combination of a rigid stalk and flexible tethers to interact with lipids, cargo, and other ESCRT complexes over a span of ∼25 nm
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