The effects of a counter-current interstitial flow on a discharging hourglass

This work experimentally investigates the effects of an interstitial fluid on the discharge of granular material within an hourglass. The experiments include observations of the flow patterns, measurements of the discharge rates, and pressure variations for a range of different fluid viscosities, particle densities and diameters, and hourglass geometries. The results are classified into three regimes: (i) granular flows with negligible interstitial fluid effects; (ii) flows affected by the presence of the interstitial fluid; and (iii) a no-flow region in which particles arch across the orifice and do not discharge. Within the fluid-affected region, the flows were visually classified as lubricated and air-coupled flows, oscillatory flows, channeling flows in which the flow preferentially rises along the sidewalls, and fluidized flows in which the upward flow suspends the particles. The discharge rates depends on the Archimedes number, the ratio of the effective hopper diameter to the particle diameter, and hourglass geometry. The hopper-discharge experiments, as well as experiments found in the literature, demonstrate that the presence of the interstitial fluid is important when the nondimensional ratio (N) of the single-particle terminal velocity to the hopper discharge velocity is less than 10. Flow ceased in all experiments in which the particle diameter was greater than 25% of the effective hopper diameter regardless of the interstitial fluid

Driving forces in free visual search : An ethology

Peer reviewedPostprin

Temporal and spatial dynamics of CO2 air-sea flux in the Gulf of Maine

Ocean surface layer carbon dioxide (CO2) data collected in the Gulf of Maine from 2004 to 2008 are presented. Monthly shipboard observations are combined with additional higher‐resolution CO2 observations to characterize CO2 fugacity ( fCO2) and CO2 flux over hourly to interannual time scales. Observed fCO2 andCO2 flux dynamics are dominated by a seasonal cycle, with a large spring influx of CO2 and a fall‐to‐winter efflux back to the atmosphere. The temporal results at inner, middle, and outer shelf locations are highly correlated, and observed spatial variability is generally small relative to the monthly to seasonal temporal changes. The averaged annual flux is in near balance and is a net source of carbon to the atmosphere over 5 years, with a value of +0.38 mol m−2 yr−1. However, moderate interannual variation is also observed, where years 2005 and 2007 represent cases of regional source (+0.71) and sink (−0.11) anomalies. We use moored daily CO2 measurements to quantify aliasing due to temporal undersampling, an important error budget term that is typically unresolved. The uncertainty of our derived annual flux measurement is ±0.26 mol m−2 yr−1 and is dominated by this aliasing term. Comparison of results to the neighboring Middle and South Atlantic Bight coastal shelf systems indicates that the Gulf of Maine exhibits a similar annual cycle and range of oceanic fCO2 magnitude but differs in the seasonal phase. It also differs by enhanced fCO2 controls by factors other than temperature‐driven solubility, including biological drawdown, fall‐to‐winter vertical mixing, and river runoff

Covalent organic frameworks

The first members of covalent organic frameworks (COF) have been designed and successfully synthesized by condensation reactions of phenyl diboronic acid C6H4[B(OH)2]2 and hexahydroxytriphenylene C18H6(OH)6. The high crystallinity of the products (C3H2BO)6 (C9H12)1 (COF-1) and C9H4BO2 (COF-5) has allowed definitive resolution of their structure by powder X-ray diffraction methods which reveal expanded porous graphitic layers that are either staggered (COF-1, P63/mmc) or eclipsed (COF-5, P6/mmm). They exhibit high thermal stability (to temperatures up to 500- to 600-C), permanent porosity, and high surface areas (711 and 1590 m2/g, respectively) surpassing those of related inorganic frameworks. A similar approach has been used for the design of other extended structures

Oblique particle-wall collisions in a liquid

This paper presents experimental measurements of the approach and rebound of a particle colliding obliquely with a wall in a viscous fluid. Steel and glass particles 12.7 mm in diameter were used. The experiments were performed using a thick Zerodur wall (a glass-like material) with various mixtures of glycerol and water. Normal and tangential coefficients of restitution were defined from the ratios of the respective velocity components at the point of contact just prior to and after impact. These coefficients account for losses due to lubrication effects and inelasticity. A third parameter, a coefficient of sliding friction, provides a measure of the tangential force acting on the particle as it slides during a collision. Oblique collisions in a fluid are qualitatively similar to oblique collisions in a dry system, with a lowered friction coefficient dependent on surface roughness. For smooth surfaces the friction coefficient is drastically reduced due to lubrication effects. A theoretical model that takes into account the dependence of viscosity on pressure is proposed to explain the observed tangential force acting on a smooth sphere during an oblique collision. The model relies on an inferred uniform temperature increase within the lubrication layer, a consequence of viscous heating during impact. The tangential force felt by the particle is expressed as a friction coefficient dependent on the viscosity within the lubrication layer. The viscosity increases owing to pressure effects and decreases owing to thermal effects. For rough surfaces the friction coefficient is comparable to that measured in dry systems, since the surface asperities may interact with each other through the lubrication layer

CD32-RNA Co-localizes with HIV-RNA in CD3+ Cells Found within Gut Tissues from Viremic and ART-Suppressed Individuals.

BackgroundIdentifying biomarkers for cells harboring replication-competent HIV is a major research priority. Recently, there have been mixed reports addressing the possibility that CD32-expressing T cells are enriched for HIV. There is growing evidence that CD32 expression increases with cellular activation that may be related to, but not necessarily specific for, infection with HIV. However, the relationship of CD32 expression to HIV-infection in subtypes of tissue-resident leukocytes is unclear.MethodsFirst, we used duplex chromogenic in situ hybridization to identify cells actively transcribing RNA for both CD32 and HIV on human gut tissues. Then we performed multiplexed immunofluorescence and in situ hybridization (mIFISH) on sections from the same tissues to determine the phenotype of individual cells co-expressing HIV-RNA and CD32-RNA.ResultsHIV-RNA+ cells were more abundant in tissues from viremic individuals than in those receiving suppressive anti-retroviral therapy (ART). However, staining by both methods indicated that a higher proportion of HIV-RNA+ cells co-expressed CD32-RNA in ART-suppressed individuals than in those with viremia. The majority of HIV-RNA+ cells were CD3+.ConclusionsOur data suggest that the transcription of CD32-RNA is correlated with HIV transcriptional activity in CD3+ cells found within human gut tissue. Whether or not up-regulation of CD32-RNA is a direct result of HIV transcription or more global T-cell activation remains unclear