2 research outputs found
LbL Assembly of Albumin on Nitric Oxide-Releasing Silica Nanoparticles Using Suramin, a Polyanion Drug, as an Interlayer Linker
Preformed protein corona of nanoparticles
can be utilized as a
promising formulation strategy for improving nano drug delivery. Nitric
oxide (NO) is a labile molecule with extensive therapeutic implications.
In this study, we test whether preformation of protein coatings can
enhance the performance of NO-delivering nanoparticles. S-Nitroso
(SNO) silica nanoparticles (SNO-SiNPs) were prepared using a nanoprecipitation
method. For the first time, bovine serum albumin (BSA) was used to
coat NO-releasing nanoparticles, facilitated by a polyanionic drug,
suramin, under a layer-by-layer (LbL) scheme. Bare and coated nanoparticles
were characterized by zeta-potential, size, and spectroscopic measurements.
We demonstrate that albumin/suramin-surface coassembly has advantages
in preventing particle aggregation during lyophilization, controlling
NO release and exerting an enhanced anticancer effect
Versatile Synthesis of Thiol- and Amine-Bifunctionalized Silica Nanoparticles Based on the Ouzo Effect
In this article, we report a novel,
nanoprecipitation-based method
for preparing silica nanoparticles with thiol and amine cofunctionalization.
(3-Mercaptopropyl)Âtrimethoxysilane (MPTMS) and 3-aminopropyltrimethoxysilane
(APTMS) were used as the organosilane precursors, which were subjected
to acid-catalyzed polycondensation in an organic phase containing
a water-miscible solvent (e.g., dimethyl sulfoxide). A pale colloidal
solution could be immediately formed when the preincubated organic
phase was directly injected into water. The initial composition ratio
between MPTMS and APTMS is an important factor governing the formation
of nanoparticles. Specifically, large, unstable micrometer-sized particles
were formed for preparation using MPTMS as the sole silane source.
In contrast, when APTMS was used alone, no particles could be formed.
By reducing the fraction of APTMS (or increasing that of MPTMS) in
the initial mixture of organosilanes, the formation of nanometer-sized
particles occurred at a critical fraction of APTMS (i.e., 25%). Remarkably,
a tiny fraction (e.g., 1%) of APTMS was sufficient to produce stable
nanoparticles with a hydrodynamic diameter of about 200 nm. Other
factors that would also affect particle formation were determined.
Moreover, an interesting temperature effect on particle formation
was observed. The TEM micrographs show spherical nanospheres with
mean sizes of 130–150 nm in diameter. The solid-state <sup>29</sup>Si NMR spectra demonstrate that the hybrid silica materials
contain fully and partially condensed silicon structures. The bifunctionalized
silica nanoparticles have positive zeta potentials whose magnitudes
are positively correlated with the amount of APTMS. The total thiol
content, however, is negatively correlated with the amount of APTMS.
The cationic nanoparticles can bind an antisense oligonucleotide in
a composition-dependent manner