Micronutrition in periodontal treatment: Antioxidants

Nutritional factors are implicated in several systemic diseases and conditions, includingoverweight and obesity, hypertension, dyslipidemia, type 2 diabetes, cardiovascular disease,osteoporosis that are associated with periodontitis. Also, periodontitis is associated with lowserum/plasma micronutrient levels. In recent years, strong evidence has emerged that dietsrich in refined carbohydrates and saturated fats are pro-inflammatory, whereas those rich inpolyunsaturated fats (fish oils), antioxidant micronutrients (fruits, berries and vegetables) areanti-inflammatory.Although numerous studies evaluated the relationship between micronutrients and periodontaldisease, intervention studies in humans are scarce. In relation to periodontal disease, numberof studies have been conducted and include vitamin C, vitamin E (tocopherol), carotenoids,polyphenols, flavonoids and other pyhtonutrients. There is a body of evidence from crosssectionalstudies that support the potential to improve periodontal outcomes by using dietaryantioxidants.This lecture will discuss the possible role of some micronutrients in the etiology and therapy ofperiodontal diseases and makes recommendations for daily nutritional intake for vitamins andantioxidants

The effects of IL-10 gene polymorphism on serum, and gingival crevicular fluid levels of IL-6 and IL-10 in chronic periodontitis

Objective Anti-inflammatory cytokines play a crucial role in periodontitis by inhibiting synthesis of pro-inflammatory cytokines. The purpose of this study was to evaluate the effect of interleukin-10 (-597) gene polymorphism and genotype distributions on chronic periodontitis (CP) development and IL-6 and IL-10 levels in gingival crevicular fluid (GCF) and serum before and after non-surgical periodontal treatment. Material and Methods The study population consisted of 55 severe generalized CP patients as CP group and 50 healthy individuals as control group. Plaque index, gingival index, probing depth and clinical attachment level were recorded and GCF and blood samples were taken at both the baseline and the sixth week after non-surgical periodontal treatment. PCR-RFLP procedure was used for gene analyses and cytokine levels were measured via ELISA. Results IL-10 genotype distribution was significantly different between CP and control groups (p=0.000, OR:7, 95%CI, 2.83-60.25). Clinical measurements significantly improved in the CP group after periodontal treatment (p;0.05). Sixth week GCF IL-10 levels were significantly lower in patients carrying IL-10 AC+CC genotype compared to the patients carrying IL-10 AA genotype (p;0.05). Conclusion IL-10 AA genotype carriers had lower IL-6 and IL-6/10 levels in serum; however, GCF IL-6/10 levels were similar in both genotypes. Within the limitations of our study, a possible association between IL-10(-597) gene polymorphism and CP might be considered

NF-κB Gen polimorfizminin agresif ve kronik periodontitis ile ilişkisi

Amaç: Transkripsiyon faktörü olan Nükleer Faktör-kappa B (NF-κB) enfeksiyona karşı immun cevapta rol alan çok çeşitli sitokinlerin gen ekspresyonunda anahtar rol oynar. Bu çalışmada kronik ve agresif periodontitis hastalarında NF-κB1(-94ins/del) gen polimorfizmi saptanması ve sağlıklı kontrol grubu ile karşılaştırılması amaçlanmıştır.Metot: Çalışmamıza 75 AgP, 72 KP ve 41 periodontal açıdan sağlıklı toplam 188 birey dahil edildi. Hastalardan plak ve gingival indeks, cep derinliği ve ataşman seviyesi ölçümleri alındı. Tüm bireylerden venöz kan örnekleri toplandı ve DNA izolasyonu yapıldı. NF-κB polimorfizmi polimeraz zincir reaksiyonu-restriksiyon parça uzunluk polimorfizmi(PZR-RFLP)  yöntemi ile belirlendi. Bulgular: Çalışmaya alınan periodontitis hasta grupları ile kontrol grubu arasında yaş ve cinsiyet açısından istatistiksel fark yoktu (p>0.05). NF-κB1 genine ait genotip dağılımları ve alel frekansları açısından AgP ve KP grupları ile kontrol grubu karşılaştırmalarında anlamlı farklılık saptanmadı (p>0.05) .Sonuçlar: Bu çalışmanın sınırları dahilinde, NF-κB1 geni(-94 ins/del) promotor polimorfizmi ile agresif ve kronik periodontitis arasında ilişki bulunmamıştır

The effect of hyperlipidemia on bone graft regeneration of peri-implantal created defects in rabbits

Abstract Aim It is reported that hyperlipidemia affects quality and density of bone and adversely affects wound healing. The effect of hyperlipidemia on implant osseointegration and peri-implant defect regeneration has not been fully explained. The purpose of this study was to examine the effects of hyperlipidemia on the healing potential of the materials used for peri-implant bone regeneration and implant stability. Materials and methods Twelve male, New Zealand rabbits were used in this study. Half of the rabbits were fed a 2% cholesterol diet for 8 weeks to induce hypercholesterolemia. Peri-implant defects (7 mm diameter) were created in the tibias of rabbits and placed implants (3.3 mm in diameter). This study was conducted as a split-mouth design. Animals were randomly divided into two groups: (1) hypercholesterol+autogenous graft group and hypercholesterol+xenograft group (n = 6), and (2) autogenous graft and xenograft groups as controls (n = 6). At 8 weeks after surgery, the rabbits were euthanized. During implant surgery and at 8 weeks, implant stability was measured with resonance frequency analysis (RFA values). Bone-to-implant contact (BIC) was analyzed via histomorphometric analysis. Results Hyperlipidemic groups showed significantly lower BIC values than those of the control groups at 8 weeks (p < 0.05). According to baseline RFA readings, there was no significant difference between control and hyperlipidemic groups (p ˃ 0.05). The hypercholesterol+autogenous graft group had significantly lower RFA readings and BIC values than the hypercholesterol+xenograft group at 8 weeks (p < 0.05). Conclusion Within the limitations of this study, it was found that hyperlipidemia may negatively affect the implant stability especially in the autogenous group and also, may decrease peri-implant bone regeneration. However, further studies are necessary to confirm these results more

Periodontoloji kliniğine başvuran hastalarda periodontal durum ve sistemik hastalıkların değerlendirilmesi

&lt;p&gt;&lt;strong&gt;Objectives:&lt;/strong&gt; The present study aims to determine the periodontal status and frequencies of the systemic diseases encountered in individuals admitted to Periodontology Department of Faculty of Dentistry at Cumhuriyet University.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Materials and Methods:&lt;/strong&gt; The present study included 1400 individuals aged between 12-82, 834 of whom were female, and 566 of whom were male. The factors investigated were periodontal status, oral hygiene habits, smoking and presence of systemic diseases. Clinical and radiological examinations were utilized in the diagnosis of periodontal disease.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Results:&lt;/strong&gt; When periodontal conditions were investigated, it was found that 0.3% of the patients were healthy, 51.9% had gingivitis, 46,4% had chronic periodontitis and 1.4% had aggressive periodontitis. The prevalence of systemic diseases in the sample group of the study was 39.2%. 31.4 % of the patients with gingivitis, 47.9% of the patients with chronic periodontitis and 50% of the patients with aggressive periodontitis were also found to have systemic disease. 2.1% of the patients included in the study had hepatitis and 24.4% were smokers.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; The prevalence of periodontal and systemic diseases in the sample group of the study was found to be high. A detailed medical history of all patients with periodontal disease should be obtained and investigated and an appropriate treatment should be planned accordingly.&lt;/p&gt;&lt;p&gt; &lt;/p&gt;&lt;p&gt;&lt;strong&gt;ÖZET&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Amaç: &lt;/strong&gt;Bu çalışmada, Cumhuriyet Üniversitesi Diş hekimliği Fakültesi Periodontoloji Anabilim Dalına başvuran bireylerin periodontal durumlarının ve bu hastalarda karşılaşılan sistemik hastalıkların sıklıklarının belirlenmesi amaçlandı.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Gereç ve Yöntem: &lt;/strong&gt;834 kadın, 566 erkek 12-82 yaş arasındaki 1400 birey çalışmaya dahil edildi. Periodontal durum, oral hijyen alışkanlıkları, sigara alışkanlığı ve sistemik hastalık varlığı araştırıldı. Klinik ve radyolojik muayene sonrası periodontal hastalık teşhisi konuldu.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Bulgular:&lt;/strong&gt; Periodontal durumlarına göre hastaların %0,3 periodontal açıdan sağlıklı, %51.9’u gingivitis, %46,4 kronik periodontitis, %1,4 agresif periodontitis olarak saptandı. Çalışmadaki bireylerin sistemik hastalık prevalansı %39.2 olarak tespit edildi. Gingivitis tanısı olan hastaların %31,4’de, kronik periodontitisli hastaların %47,9 ve agresif periodontitisli hastaların %50'sinde sistemik hastalık gözlendi. Bu çalışmaya katılan bireylerin %2,1’nin hepatit olduğu ve  %24,4’nün sigara içtiği tespit edildi.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sonuçlar: &lt;/strong&gt;Bu çalışma, periodontal ve sistemik hastalıkların görülme sıklığının incelenen bireyler arasında yüksek olduğunu gösterdi. Periodontal hastalığı bulunan tüm bireylerden tedavi öncesi detaylı anamnez alınmalı, buna göre uygun tedavi planlanmalıdır.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Anahtar Kelimeler: &lt;/strong&gt;Periodontal hastalık, tıbbi öykü, sistemik hastalık.&lt;strong&gt; &lt;/strong&gt;&lt;/p&gt

Prevalence of periodontal disease in patients with Familial Mediterranean Fever: A cohort study from central Turkey

Objective: The aim of this study was to compare the periodontal status in patients with Familial Mediterranean Fever (FMF) and in those without this disease. Method and Materials: 84 subjects clinically diagnosed with FMF and 75 systemically healthy controls, matched by age and gender, were recruited. All FMF patients were on a regular daily colchicine treatment and during attack-free periods. Gingival Index (GI), Plaque Index (PI), probing pocket depth (PD), and clinical attachment level (CAL) were measured in all subjects. To evaluate periodontal disease further, patients were stratified into five groups. Education information and smoking habits were recorded. Results: The FMF patients and healthy controls were comparable for age, gender, and smoking status (P > .05). The FMF patients had significantly higher PI and GI values and lower PD and CAL values than those of the control group (P .05). In the FMF-severe periodontitis group, higher PI and GI values were seen (P .05). Conclusion: Patients with FMF using colchicine did not manifest higher attachment loss compared to age- and sex-matched systemically healthy controls.OBJECTIVE:The aim of this study was to compare the periodontal status in patients with Familial Mediterranean Fever (FMF) and in those without this disease.METHOD AND MATERIALS:84 subjects clinically diagnosed with FMF and 75 systemically healthy controls, matched by age and gender, were recruited. All FMF patients were on a regular daily colchicine treatment and during attack-free periods. Gingival Index (GI), Plaque Index (PI), probing pocket depth (PD), and clinical attachment level (CAL) were measured in all subjects. To evaluate periodontal disease further, patients were stratified into fi ve groups. Education information and smoking habits were recorded.RESULTS:The FMF patients and healthy controls were comparable for age, gender, and smoking status (P&gt;.05). The FMF patients had significantly higher PI and GI values and lower PD and CAL values than those of the control group (P&lt;.05). However, there was no significant difference among all groups in terms of periodontal disease severity (P&gt;.05). In the FMF-severe periodontitis group, higher PI and GI values were seen (P&lt;.05). However, there was no significant difference between the FMF-severe periodontitis group and the controls with severe periodontitis regarding the PD and CAL values (P&gt;.05).CONCLUSION:Patients with FMF using colchicine did not manifest higher attachment loss compared to age- and sex-matched systemically healthy controls.</p

Effect of Chronic Periodontitis on Serum and Gingival Crevicular Fluid Oxidant and Antioxidant Status in Patients With Familial Mediterranean Fever Before and After Periodontal Treatment

Background: The aim of this study is to investigate the impact of periodontal status on oxidant/antioxidant status in patients with chronic periodontitis (CP) who experienced familial Mediterranean fever (FMF) and their response to non-surgical periodontal therapy.Background: The aim of this study is to investigate the impact of periodontal status on oxidant/antioxidant status in patients with chronic periodontitis (CP) who experienced familial Mediterranean fever (FMF) and their response to non-surgical periodontal therapy.Methods: Data were obtained from 13 patients with FMF with generalized CP (FMF-CP), 15 systemically healthy patients with generalized CP, 15 systemically and periodontal healthy controls (HCs), and 14 periodontally healthy patients with FMF (FMF-HC). Each participant&rsquo;s total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) in their gingival crevicular fluid (GCF) and serum were recorded. Probing depth, clinical attachment level, and gingival and plaque indices in each participant were also measured. The GCF and clinical parameters at baseline and 6 weeks after periodontal treatment were recorded.Results: The study showed statistically significant improvement of clinical parameters in both FMF-CP and CP groups after periodontal treatment. The baseline GCF-TOS and OSI levels were significantly higher in the CP group compared with the FMF-CP group (P &lt;0.05). After periodontal treatment, the GCF-TOS levels were significantly reduced in members of the FMF-CP group (P &lt;0.05). The GCF-TAS levels in members of the FMF-CP group were significantly higher than those of members of the HC group at baseline (P &lt;0.05). Serum-TAS levels in the FMF-CP group were significantly higher than those in the CP and HC groups at baseline (P &lt;0.05). The GCF-TOS level in the FMF-CP group was significantly higher than that in the FMF-HC group at baseline and 6 weeks. However, there were no significant differences in the serum-TOS and serum-OSI levels of those in the FMF-CP and CP groups at baseline and 6 weeks (P &gt;0.05).Conclusion: The results of the present study show that patients with FMF-CP displayed reduced oxidative stress and increased antioxidant status compared with those in the CP and HC groups.</p