Impact of Schistosoma japonicum Infection on Collagen-Induced Arthritis in DBA/1 Mice: A Murine Model of Human Rheumatoid Arthritis

BACKGROUND: The hygiene hypothesis suggests that helminth infections prevent a range of autoimmune diseases. METHODOLOGY/PRINCIPAL FINDINGS: To investigate the effects of S. japonicum infection on collagen-induced arthritis (CIA), male DBA/1 mice were challenged with unisexual or bisexual S. japonicum cercariae two weeks prior to bovine type II collagen (CII) immunization or at the onset of CIA. S. japonicum infection prior to CII immunization significantly reduced the severity of CIA. ELISA (enzyme linked immunosorbent assay) showed that the levels of anti-CII IgG and IgG2a were reduced in prior schistosome-infected mice, while anti-CII IgG1 was elevated. Splenocyte proliferation against both polyclonal and antigen-specific stimuli was reduced by prior schistosome infection as measured by tritiated thymidine incorporation ((3)H-TdR). Cytokine profiles and CD4(+) T cells subpopulation analysis by ELISA and flow cytometry (FCM) demonstrated that prior schistosome infection resulted in a significant down-regulation of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β and IL-6) and Th1 cells, together with up-regulation of the anti-inflammatory cytokine IL-10 and Th2 cells. Interestingly, the expansion of Treg cells and the reduction of Th17 cells were only observed in bisexually infected mice. In addition, prior schistosome infection notably reduced the expression of pro-inflammatory cytokines and receptor activator of NF-κB ligand (RANKL) in the inflamed joint. However, the disease was exacerbated at one week after infection when established CIA mice were challenged with bisexual cercariae. CONCLUSION/SIGNIFICANCE: Our data provide direct evidence that the Th2 response evoked by prior S. japonicum infection can suppress the Th1 response and pro-inflammatory mediator and that bisexual infection with egg-laying up-regulates the Treg response and down-regulates the Th17 response, resulting in an amelioration of autoimmune arthritis. The beneficial effects might depend on the establishment of a Th2-dominant response rather than the presence of the eggs. Our results suggest that anti-inflammatory molecules from the parasite could treat autoimmune diseases

Quantitative Correlation between Thermal Cycling and the Microstructures of X100 Pipeline Steel Laser-Welded Joints

Due to the limitations of the energy density and penetration ability of arc welding technology for long-distance pipelines, the deterioration of the microstructures in the coarse-grained heat-affected zone (HAZ) in welded joints in large-diameter, thick-walled pipeline steel leads to insufficient strength and toughness in these joints, which strongly affect the service reliability and durability of oil and gas pipelines. Therefore, high-energy-beam welding is introduced for pipeline steel welding to reduce pipeline construction costs and improve the efficiency and safety of oil and gas transportation. In the present work, two pieces of X100 pipeline steel plates with thicknesses of 12.8 mm were welded by a high-power robot laser-welding platform. The quantitative correlation between thermal cycling and the microstructure of the welded joint was studied using numerical simulation of the welding temperature field, optical microscopy (OM), and scanning electron microscopy (SEM) with energy-dispersive spectroscopy (EDS). The results show that the heat-source model of a Gaussian-distributed rotating body and the austenitization degree parameters are highly accurate in simulating the welding temperature field and characterizing the austenitization degree. The effects of austenitization are more significant than those of the cooling rate on the final microstructures of the laser-welded joint. The microstructure of the X100 pipeline steel in the HAZ is mainly composed of acicular ferrite (AF), granular bainite (GB), and bainitic ferrite (BF). However, small amounts of lath martensite (LM), upper bainite (UB), and the bulk microstructure are found in the columnar zone of the weld. The aim of this paper is to provide scientific guidance and a reference for the simulation of the temperature field during high-energy-beam laser welding and to study and formulate the laser-welding process for X100 pipeline steel. Document type: Articl

Data from a comparative proteomic analysis of tumor-derived lung-cancer CD105+ endothelial cells

AbstractIncreasing evidence indicates that tumor-derived endothelial cells (TECs) are more relevant for the study of tumor angiogenesis and for screening antiangiogenic drugs than normal ECs (NECs). In this data article, high-purity (>98%) primary CD105+ NECs and TECs purified from a mouse Lewis lung carcinoma model bearing 0.5cm tumors were identified using 2D-PAGE and Matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS). All the identified proteins were categorized functionally by Gene Ontology (GO) analysis, and gene-pathway annotated by Kyoto Encyclopedia of Genes and Genomes (KEGG). Finally, protein–protein interaction networks were also built. The proteomics and bioinformatics data presented here provide novel insights into the molecular characteristics and the early modulation of the TEC proteome in the tumor microenvironment

The Organization of Sex Work in Low- and High-Priced Venues with a Focus on the Experiences of Ethnic Minority Women Working in These Venues

Prior research on female sex workers (FSW) in China, and their risk for HIV and STI, neglects the nuanced experiences of ethnic minority FSW. We conducted participant observations and in-depth interviews with 33 FSW and six venue bosses to describe the experiences of FSW and management structures in high and low-priced sex work venues in Liuzhou, China. In low-priced venues, FSW had more autonomy and stronger relationships with their ethnic minority peers. Mid and high-priced venues had more formal management structures. Ethnic minority FSW working in higher priced venues experienced less support and kinship with their peers. HIV/STI prevention outreach activities occurred in all of the venues, but they were not tailored for different venue types or for ethnic minority FSW. Our findings provide guidance for tailoring public health programs that meet the needs of ethnic minority women working in different types of sex work venues

Cloning, expression and characterization of alcohol dehydrogenases in the silkworm Bombyx mori

Alcohol dehydrogenases (ADH) are a class of enzymes that catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones, by using either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), as coenzymes. In this study, a short-chain ADH gene was identified in Bombyx mori by 5′-RACE PCR. This is the first time the coding region of BmADH has been cloned, expressed, purified and then characterized. The cDNA fragment encoding the BmADH protein was amplified from a pool of silkworm cDNAs by PCR, and then cloned into E. coli expression vector pET-30a(+). The recombinant His-tagged BmADH protein was expressed in E. coli BL21 (DE3), and then purified by metal chelating affinity chromatography. The soluble recombinant BmADH, produced at low-growth temperature, was instrumental in catalyzing the ethanol-dependent reduction of NAD+, thereby indicating ethanol as one of the substrates of BmADH

A Stimuli-Responsive Biosensor of Glucose on Layer-by-Layer Films Assembled through Specific Lectin-Glycoenzyme Recognition

The research on intelligent bioelectrocatalysis based on stimuli-responsive materials or interfaces is of great significance for biosensors and other bioelectronic devices. In the present work, lectin protein concanavalin A (Con A) and glycoenzyme glucose oxidase (GOD) were assembled into {Con A/GOD}n layer-by-layer (LbL) films by taking advantage of the biospecific lectin-glycoenzyme affinity between them. These film electrodes possess stimuli-responsive properties toward electroactive probes such as ferrocenedicarboxylic acid (Fc(COOH)2) by modulating the surrounding pH. The CV peak currents of Fc(COOH)2 were quite large at pH 4.0 but significantly suppressed at pH 8.0, demonstrating reversible stimuli-responsive on-off behavior. The mechanism of stimuli-responsive property of the films was explored by comparative experiments and attributed to the different electrostatic interaction between the films and the probes at different pH. This stimuli-responsive films could be used to realize active/inactive electrocatalytic oxidation of glucose by GOD in the films and mediated by Fc(COOH)2 in solution, which may establish a foundation for fabricating novel stimuli-responsive electrochemical biosensors based on bioelectrocatalysis with immobilized enzymes

Environmental Regulation, Roundabout Production, and Industrial Structure Transformation and Upgrading: Evidence from China

By dividing the production process into technical processes and procedural processes, this paper supplements the theoretical connotation of production roundaboutness, and analyzes and discusses the micro impact mechanisms of environmental regulation on industrial structure through production roundaboutness. Subsequently, based on the panel data of 31 provinces and cities in China from 2003 to 2017, a panel threshold model was adopted to empirically analyze the impact of environmental regulation, the synergy of environmental regulation, and roundabout production on the transformation and upgrading of industrial structures. The result shows there is a single threshold effect of environmental regulation on the upgrading and transformation of industrial structures based on the threshold variable of production roundaboutness. As well as the continuous increase in production roundaboutness, significant positive correlations between environmental regulation and the transformation and upgrading of industrial structures gradually vanish. However, the synergistic growth of environmental regulation and production roundaboutness is significantly and negatively correlated with industrial structure transformation and upgrade. Investment in social fixed assets has a positive incentive effect on industrial structure transformation and upgrade, while government expenditure, foreign trade, and opening-up have different degrees of inhibition on it. In order to speed up the transformation and upgrading of industrial structures under environmental and resource constraints, it is necessary to straighten out the relationship between environmental regulation and roundabout production, and to promote the scientific development of both

Application and Analysis of Curtain Grouting in Seepage Prevention and Reinforcement of Masonry Dam

At present, the number of masonry dam in China is still relatively large. Due to various reasons, many of the dam body appears aging, degradation and other phenomena, and leakage is one of the common problems. Now grouting technology is the main technical means of anti-seepage treatment. Curtain grouting technology has wide adaptability to strata. With the gradual improvement of grouting technology, it has been widely applied in engineering and water conservancy projects. In order to ensure the safety of the dam and improve the grouting effect, this paper summarizes the rules of anti-seepage treatment based on the actual grouting treatment methods, and provides technical guidance for the anti-seepage treatment of other similar masonry dams

Rank-Constrained Beamforming for MIMO Cognitive Interference Channel

This paper considers the spectrum sharing multiple-input multiple-output (MIMO) cognitive interference channel, in which multiple primary users (PUs) coexist with multiple secondary users (SUs). Interference alignment (IA) approach is introduced that guarantees that secondary users access the licensed spectrum without causing harmful interference to the PUs. A rank-constrained beamforming design is proposed where the rank of the interferences and the desired signals is concerned. The standard interferences metric for the primary link, that is, interference temperature, is investigated and redesigned. The work provides a further improvement that optimizes the dimension of the interferences in the cognitive interference channel, instead of the power of the interference leakage. Due to the nonconvexity of the rank, the developed optimization problems are further approximated as convex form and are solved via choosing the transmitter precoder and receiver subspace iteratively. Numerical results show that the proposed designs can improve the achievable degree of freedom (DoF) of the primary links and provide the considerable sum rate for both secondary and primary transmissions under the rank constraints