Characterization, dissolution and solubility of the hydroxypyromorphite–hydroxyapatite solid solution [(PbxCa1−x)5(PO4)3OH] at 25 °C and pH 2–9

Additional file 1: Appendix A. Supplementary data—X-ray diffractograms (XRD) of the hydroxypyromorphite–hydroxyapatite solid solution [(PbxCa1−x)5(PO4)3(OH)] after dissolution at 25 ˚C and an initial pH of 5.60 and 9.00 for 300d

Crowd3D: Towards Hundreds of People Reconstruction from a Single Image

Image-based multi-person reconstruction in wide-field large scenes is critical for crowd analysis and security alert. However, existing methods cannot deal with large scenes containing hundreds of people, which encounter the challenges of large number of people, large variations in human scale, and complex spatial distribution. In this paper, we propose Crowd3D, the first framework to reconstruct the 3D poses, shapes and locations of hundreds of people with global consistency from a single large-scene image. The core of our approach is to convert the problem of complex crowd localization into pixel localization with the help of our newly defined concept, Human-scene Virtual Interaction Point (HVIP). To reconstruct the crowd with global consistency, we propose a progressive reconstruction network based on HVIP by pre-estimating a scene-level camera and a ground plane. To deal with a large number of persons and various human sizes, we also design an adaptive human-centric cropping scheme. Besides, we contribute a benchmark dataset, LargeCrowd, for crowd reconstruction in a large scene. Experimental results demonstrate the effectiveness of the proposed method. The code and datasets will be made public.Comment: 8 pages (not including reference

Biosynthesis, characterization, and antifungal activity of plant-mediated silver nanoparticles using Cnidium monnieri fruit extract

The present study describes a novel method for green synthesis of silver nanoparticles using Cnidium monnieri (CM-AgNPs). Cnidium monnieri fruit is an excellent anti tinea drug that can be used externally to treat superficial fungal infections in the human body. The aqueous ethanolic extract of Cnidium monnieri fruit was prepared and employed in the synthesis of stable silver nanoparticles via biological reduction method. The synthesis conditions of CM-AgNPs was systematically optimized using Box–Behnken design. CM-AgNPs were well characterized by UV-spectroscopy and X-ray powder diffraction (XRD), and it was confirmed that the synthesized particles were AgNPs. The possible functional groups required for the reduction and stabilization of CM-AgNPs in the extract were identified through FTIR spectrum. The size of CM-AgNPs structure was confirmed to be approximately 44.6 nm in polydisperse spherical shape through scanning electron microscopy (SEM), transmission electron microscopy (TEM), and laser dynamic light scattering (DLS). Further, the minimum inhibitory concentration 90% (MIC90) ratios values of Cm-AgNPs against Trichophyton rubrum (7 d), T. mentagrophytes (7 d) and Candida albicans (24 h) were 3.125, 3.125, and 0.78125 μg/mL, respectively, determined by the broth micro dilution method. Finally, the result was concluded that the synthesized AgNPs could be further evaluated in large scale as a potential human topical antifungal agent

Assessing the effects of trans-boundary aerosol transport between various city clusters on regional haze episodes in spring over East China

Regional haze episodes have been frequently reported in east China since 2000. In the present study, two regional haze episodes over east China in the spring of 2011 were examined by observations and simulations conducted by a three-dimensional regional chemical transport model (NAQPMS) with an on-line tracer-tagged module. The model reproduced accurately the observed PM2.5 with correlation coefficient ranging from 0.52 to 0.76 and root mean square error (RMSE) of 20–50µg/m3 in four city clusters (Yangtze River Delta, Shandong Peninsula, Huabei Plain and Central Liaoning) over east China. Our results indicate that a northward cross-border transport from the Yangtze River Delta to Central Liaoning below 2 km above ground played an important role in the formation of these regional high PM2.5 episodes. Contributions of regional transport from outside city clusters presented an increasing trend from south to north. In the northernmost cluster (Central Liaoning), the contribution from other city clusters reached 40–50% during the two episodes. In contrast, it was below 10% in the Yangtze River Delta (southernmost cluster). Mixing accumulation of pollutants from various city clusters during transport was responsible for this trend. Furthermore, a preliminary estimate shows that cross-border transport of PM2.5 might increase 0.5–3% daily mortality during the high PM2.5 episodes

Effect of simulating parity-odd observables in high energy heavy ion collisions on Balance Functions of charged particles and elliptic flow of pions

At the early stage of heavy ion collisions, non-trivial topologies of the gauge fields can be created resulting in an imbalance of axial charge density and eventually separation of electric charges along the direction of the magnetic field produced in such collisions. This process is called the chiral magnetic effect (CME). In this work we implement such a charge separation at the partonic level in AMPT for Au+Au collisions at $\sqrt{s_{NN}}$ = 200 GeV to study its consequence on experimental observables. We present the effects on the pion elliptic flow ($v_2$) and the charged particle balance function (BF) for varying strengths of initial charge separation. We find that the shape of the balance function is sensitive to the increasing charge separation. $v_2$ of pion shows a strong decreasing trend at higher transverse momenta ($p_T$) with increasing charge separation. Charge balance functions show a peak at $\Delta\phi \sim 180$ with charge separation implemented in the partonic level as expected for the parity violation. We have also calculated parity observable $\gamma$ in the form of BF's moments. $\gamma$ shows a decreasing trend with charge separation. It has a negative value for charge separation produced by flipping more than 30 $\%$ of quarks in the parton level. We also notice that $<\gamma>$ for the same charge correlation and the opposite charge correlation shows negative and positive values, respectively

Neurotoxicological effects induced by up-regulation of miR-137 following triclosan exposure to zebrafish (Danio rerio).

Triclosan (TCS) is a prevalent anthropogenic contaminant in aquatic environments and its chronic exposure can lead to a series of neurotoxic effects in zebrafish. Both qRT-PCR and W-ISH identified that TCS exposure resulted in significant up-regulation of miR-137, but downregulation of its regulatory genes (bcl11aa, MAPK6 and Runx1). These target genes are mainly associated with neurodevelopment and the MAPK signaling pathway, and showed especially high expression in the brain. After overexpression or knockdown treatments by manual intervention of miR-137, a series of abnormalities were induced, such as ventricular abnormality, bent spine, yolk cyst, closure of swim sac and venous sinus hemorrhage. The most sensitive larval toxicological endpoint from intervened miR-137 expression was impairment of the central nervous system (CNS), ventricular abnormalities and notochord curvature. Microinjection of microRNA mimics or inhibitors of miR-137 both caused zebrafish malformations. The posterior lateral line neuromasts became obscured and decreased in number in intervened miR-137 groups and TCS-exposure groups. Up-regulation of miR-137 led to more severe neurotoxic effects than its down-regulation. Behavioral observations demonstrated that both TCS exposure and miR-137 over-expression led to inhibited hearing or vision sensitivity. HE staining indicated that hearing and vision abnormalities induced by long-term TCS exposure originated from CNS injury, such as reduced glial cells and loose and hollow fiber structures. The findings of this study enhance our mechanistic understanding of neurotoxicity in aquatic animals in response to TCS exposure. These observations provide theoretical guidance for development of early intervention treatments for nervous system diseases

The Mechanism of ATP-Dependent Allosteric Protection of Akt Kinase Phosphorylation

SummaryKinases use ATP to phosphorylate substrates; recent findings underscore the additional regulatory roles of ATP. Here, we propose a mechanism for allosteric regulation of Akt1 kinase phosphorylation by ATP. Our 4.7-μs molecular dynamics simulations of Akt1 and its mutants in the ATP/ADP bound/unbound states revealed that ATP occupancy of the ATP-binding site stabilizes the closed conformation, allosterically protecting pT308 by restraining phosphatase access and key interconnected residues on the ATP→pT308 allosteric pathway. Following ATP→ADP hydrolysis, pT308 is exposed and readily dephosphorylated. Site-directed mutagenesis validated these predictions and indicated that the mutations do not impair PDK1 and PP2A phosphatase recruitment. We further probed the function of residues around pT308 at the atomic level, and predicted and experimentally confirmed that Akt1H194R/R273H double mutant rescues pathology-related Akt1R273H. Analysis of classical Akt homologs suggests that this mechanism can provide a general model of allosteric kinase regulation by ATP; as such, it offers a potential avenue for allosteric drug discovery