51 research outputs found
Die Bedeutung der Temperatur bei der Kultur des Champignons insbesondere beim "Till-Verfahren"
Die III. Phase der Entwicklung des Champignon-Anbauverfahrens auf nicht kompostiertem sterilem Nährsubstrat
Neues Verfahren der industriellen und nicht industriellen Brutherstellung fĂĽr die Produktion von Speisepilzen auf der Basis von fermentiertem Substrat
Together is Better: mRNA Co-Encapsulation in Lipoplexes is Required to Obtain Ratiometric Co-Delivery and Protein Expression on the Single Cell Level
Liposomes can efficiently deliver messenger RNA (mRNA) into cells. When mRNA cocktails encoding different proteins are needed, a considerable challenge is to efficiently deliver all mRNAs into the cytosol of each individual cell. In this work, two methods are explored to co-deliver varying ratiometric doses of mRNA encoding red (R) or green (G) fluorescent proteins and it is found that packaging mRNAs into the same lipoplexes (mingle-lipoplexes) is crucial to efficiently deliver multiple mRNA types into the cytosol of individual cells according to the pre-defined ratio. A mixture of lipoplexes containing only one mRNA type (single-lipoplexes), however, seem to follow the “first come – first serve” principle, resulting in a large variation of R/G uptake and expression levels for individual cells leading to ratiometric dosing only on the population level, but rarely on the single-cell level. These experimental observations are quantitatively explained by a theoretical framework based on the stochasticity of mRNA uptake in cells and endosomal escape of mingle- and single-lipoplexes, respectively. Furthermore, the findings are confirmed in 3D retinal organoids and zebrafish embryos, where mingle-lipoplexes outperformed single-lipoplexes to reliably bring both mRNA types into single cells. This benefits applications that require a strict control of protein expression in individual cells
Influence of Internal Baffles on Mixing Characteristics of Biomass in a Fluidized Sand Bed
Rosana G. Moreira, Editor-in-Chief; Texas A&M UniversityThis is a paper from International Commission of Agricultural Engineering (CIGR, Commission Internationale du Genie Rural) E-Journal Volume 9 (2007): Influence of Internal Baffles on Mixing Characteristics of Biomass in a Fluidized Sand Bed. Manuscript EE 06 016. Vol. IX. April, 2007
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