Deformation conditions during syn-convergent extension along the Cordillera Blanca shear zone, Peru

Strain localization across the brittle-ductile transition is a fundamental process in accommodating tectonic movement in the mid-crust. The tectonically active Cordillera Blanca shear zone (CBSZ), a ~200-km-long normal-sense shear zone situated within the footwall of a discrete syn-convergent extensional fault in the Peruvian Andes, is an excellent field laboratory to explore this transition. Field and microscopic observations indicate consistent top-down-to-the-southwest sense of shear and a sequence of tectonites ranging from undeformed granodiorite through mylonite and ultimately fault breccia along the detachment. Using microstructural analysis, two-feldspar and Ti-in-quartz (TitaniQ) thermometry, recrystallized quartz paleopiezometry, and analysis of quartz crystallographic preferred orientations, we evaluate the deformation conditions and mechanisms in quartz and feldspar across the CBSZ. Deformation temperatures derived from asymmetric strain-induced myrmekite in a subset of tectonite samples are 410 ± 30 to 470 ± 36 °C, consistent with TitaniQ temperatures of 450 ± 60 to 490 ± 33 °C and temperatures \u3e400 °C estimated from microstructural criteria. Brittle fabrics overprint ductile fabrics within ~150 m of the detachment that indicate that deformation continued to lower-temperature (~280–400 °C) and/or higher-strain-rate conditions prior to the onset of pervasive brittle deformation. Initial deformation occurred via high-temperature fracturing and dissolution-precipitation in feldspar. Continued subsolidus deformation resulted in either layering of mylonites into monophase quartz and fine-grained polyphase domains oriented subparallel to macroscopic foliation or the interconnection of recrystallized quartz networks oriented obliquely to macroscopic foliation. The transition to quartz-controlled rheology occurred at temperatures near ~500 °C and at a differential stress of ~16.5 MPa. Deformation within the CBSZ occurred predominantly above ~400 °C and at stresses up to ~71.4 MPa prior to the onset of brittle deformation

Deformation Conditions During Syn-Convergent Extension Along the Cordillera Blanca Shear Zone, Peru

Strain localization across the brittle-ductile transition is a fundamental process in accommodating tectonic movement in the mid-crust. The tectonically active Cordillera Blanca shear zone (CBSZ), a ∼200-km-long normal-sense shear zone situated within the footwall of a discrete syn-convergent extensional fault in the Peruvian Andes, is an excellent field laboratory to explore this transition. Field and microscopic observations indicate consistent top-down-to-the-southwest sense of shear and a sequence of tectonites ranging from undeformed granodiorite through mylonite and ultimately fault breccia along the detachment. Using microstructural analysis, two-feldspar and Ti-in-quartz (TitaniQ) thermometry, recrystallized quartz paleopiezometry, and analysis of quartz crystallographic preferred orientations, we evaluate the deformation conditions and mechanisms in quartz and feldspar across the CBSZ. Deformation temperatures derived from asymmetric strain-induced myrmekite in a subset of tectonite samples are 410 ± 30 to 470 ± 36 °C, consistent with TitaniQ temperatures of 450 ± 60 to 490 ± 33 °C and temperatures \u3e400 °C estimated from microstructural criteria. Brittle fabrics overprint ductile fabrics within ∼150 m of the detachment that indicate that deformation continued to lower-temperature (∼280–400 °C) and/or higher-strain-rate conditions prior to the onset of pervasive brittle deformation. Initial deformation occurred via high-temperature fracturing and dissolution-precipitation in feldspar. Continued subsolidus deformation resulted in either layering of mylonites into monophase quartz and fine-grained polyphase domains oriented subparallel to macroscopic foliation or the interconnection of recrystallized quartz networks oriented obliquely to macroscopic foliation. The transition to quartz-controlled rheology occurred at temperatures near ∼500 °C and at a differential stress of ∼16.5 MPa. Deformation within the CBSZ occurred predominantly above ∼400 °C and at stresses up to ∼71.4 MPa prior to the onset of brittle deformation

Sequencing and characterization of the guppy (Poecilia reticulata) transcriptome

Background: Next-generation sequencing is providing researchers with a relatively fast and affordable option for developing genomic resources for organisms that are not among the traditional genetic models. Here we present a de novo assembly of the guppy (Poecilia reticulata) transcriptome using 454 sequence reads, and we evaluate potential uses of this transcriptome, including detection of sex-specific transcripts and deployment as a reference for gene expression analysis in guppies and a related species. Guppies have been model organisms in ecology, evolutionary biology, and animal behaviour for over 100 years. An annotated transcriptome and other genomic tools will facilitate understanding the genetic and molecular bases of adaptation and variation in a vertebrate species with a uniquely well known natural history. Results: We generated approximately 336 Mbp of mRNA sequence data from male brain, male body, female brain, and female body. The resulting 1,162,670 reads assembled into 54,921 contigs, creating a reference transcriptome for the guppy with an average read depth of 28×. We annotated nearly 40% of this reference transcriptome by searching protein and gene ontology databases. Using this annotated transcriptome database, we identified candidate genes of interest to the guppy research community, putative single nucleotide polymorphisms (SNPs), and male-specific expressed genes. We also showed that our reference transcriptome can be used for RNA- sequencing-based analysis of differential gene expression. We identified transcripts that, in juveniles, are regulated differently in the presence and absence of an important predator, Rivulus hartii, including two genes implicated in stress response. For each sample in the RNA-seq study, >50% of high-quality reads mapped to unique sequences in the reference database with high confidence. In addition, we evaluated the use of the guppy reference transcriptome for gene expression analyses in a congeneric species, the sailfin molly (Poecilia latipinna). Over 40% of reads from the sailfin molly sample aligned to the guppy transcriptome. Conclusions: We show that next-generation sequencing provided a reliable and broad reference transcriptome. This resource allowed us to identify candidate gene variants, SNPs in coding regions, and sex-specific gene expression, and permitted quantitative analysis of differential gene expression

Patients with aortic stenosis exhibit early improved endothelial function following transcatheter aortic valve replacement: The eFAST study

BACKGROUND: Patients with severe aortic stenosis (AS) exhibit systemic endothelial dysfunction, which can be associated with myocardial ischaemia in absence of obstructive coronary disease. Transcatheter aortic valve replacement (TAVR) is used to treat severe AS in patients with high or prohibitive surgical risk. However, it remains unknown whether endothelial function recovers post-TAVR. We therefore sought to assess the early and late changes in flow-mediated dilation (FMD), a measure of endothelial function, following TAVR. METHODS: Patients undergoing TAVR for severe AS had ultrasound assessment of brachial endothelial-independent and -dependent FMD. Measurements were performed pre-TAVR, at early follow-up (<48 h post-TAVR) and late follow-up (4-6 weeks post-TAVR). RESULTS: 27 patients (mean age 82.0 ± 7.0; 33.3% female) were recruited; 37.0% had diabetes mellitus and 59.3% had hypertension. Brachial artery FMD increased from 4.2 ± 1.6% (pre-TAVR) to 9.7 ± 3.5% at early follow-up (p < 0.0001). At late follow-up, improvement compared with early follow-up was sustained (8.7 ± 1.9%, p = 0.27). Resting brachial arterial flow velocities decreased significantly at late follow-up (11.24 ± 5.16 vs. 7.73 ± 2.79 cm/s, p = 0.003). Concordantly, at late follow-up, there was decrease in resting wall shear stress (WSS; 14.8 ± 7.8 vs. 10.6 ± 4.8dyne/cm2, p = 0.01), peak WSS (73.1 ± 34.1 vs. 58.8 ± 27.8dyne/cm2, p = 0.03) and cumulative WSS (3543 ± 1852 vs. 2504 ± 1089dyne·s/cm2, p = 0.002). Additionally, a favourable inverse correlation between cumulative WSS and FMD was restored at late follow-up (r = -0.21 vs. r = 0.49). CONCLUSION: Endothelial function in patients with AS improves early post-TAVR and this improvement is sustained. This likely occurs as a result of improved arterial haemodynamics, leading to lower localised WSS and release of vasoactive mediators that may also alleviate myocardial ischaemia

Steiner t-designs for large t

One of the most central and long-standing open questions in combinatorial design theory concerns the existence of Steiner t-designs for large values of t. Although in his classical 1987 paper, L. Teirlinck has shown that non-trivial t-designs exist for all values of t, no non-trivial Steiner t-design with t > 5 has been constructed until now. Understandingly, the case t = 6 has received considerable attention. There has been recent progress concerning the existence of highly symmetric Steiner 6-designs: It is shown in [M. Huber, J. Algebr. Comb. 26 (2007), pp. 453-476] that no non-trivial flag-transitive Steiner 6-design can exist. In this paper, we announce that essentially also no block-transitive Steiner 6-design can exist.Comment: 9 pages; to appear in: Mathematical Methods in Computer Science 2008, ed. by J.Calmet, W.Geiselmann, J.Mueller-Quade, Springer Lecture Notes in Computer Scienc

Feasibility and Validity of Computed Tomography-Derived Fractional Flow Reserve in Patients With Severe Aortic Stenosis: The CAST-FFR Study

BACKGROUND: Coronary artery disease is common in patients with severe aortic stenosis. Computed tomography-derived fractional flow reserve (CT-FFR) is a clinically used modality for assessing coronary artery disease, however, its use has not been validated in patients with severe aortic stenosis. This study assesses the safety, feasibility, and validity of CT-FFR in patients with severe aortic stenosis. METHODS: Prospectively recruited patients underwent standard-protocol invasive FFR and coronary CT angiography (CTA). CTA images were analyzed by central core laboratory (HeartFlow, Inc) for independent evaluation of CT-FFR. CT-FFR data were compared with FFR (ischemia defined as FFR ≤0.80). RESULTS: Forty-two patients (68 vessels) underwent FFR and CTA; 39 patients (92.3%) and 60 vessels (88.2%) had interpretable CTA enabling CT-FFR computation. Mean age was 76.2±6.7 years (71.8% male). No patients incurred complications relating to premedication, CTA, or FFR protocol. Mean FFR and CT-FFR were 0.83±0.10 and 0.77±0.14, respectively. CT calcium score was 1373.3±1392.9 Agatston units. On per vessel analysis, there was positive correlation between FFR and CT-FFR (Pearson correlation coefficient, R=0.64, P<0.0001). Sensitivity, specificity, positive predictive value, and negative predictive values were 73.9%, 78.4%, 68.0%, and 82.9%, respectively, with 76.7% diagnostic accuracy. The area under the receiver-operating characteristic curve for CT-FFR was 0.83 (0.72-0.93, P<0.0001), which was higher than that of CTA and quantitative coronary angiography (P=0.01 and P<0.001, respectively). Bland-Altman plot showed mean bias between FFR and CT-FFR as 0.059±0.110. On per patient analysis, the sensitivity, specificity, positive predictive, and negative predictive values were 76.5%, 77.3%, 72.2%, and 81.0% with 76.9% diagnostic accuracy. The per patient area under the receiver-operating characteristic curve analysis was 0.81 (0.67-0.95, P<0.0001). CONCLUSIONS: CT-FFR is safe and feasible in patients with severe aortic stenosis. Our data suggests that the diagnostic accuracy of CT-FFR in this cohort potentially enables its use in clinical practice and provides the foundation for future research into the use of CT-FFR for coronary evaluation pre-aortic valve replacement

Random walk generated by random permutations of {1,2,3, ..., n+1}

We study properties of a non-Markovian random walk $X^{(n)}_l$, $l =0,1,2, >...,n$, evolving in discrete time $l$ on a one-dimensional lattice of integers, whose moves to the right or to the left are prescribed by the \text{rise-and-descent} sequences characterizing random permutations $\pi$ of $[n+1] = \{1,2,3, ...,n+1\}$. We determine exactly the probability of finding the end-point $X_n = X^{(n)}_n$ of the trajectory of such a permutation-generated random walk (PGRW) at site $X$, and show that in the limit $n \to \infty$ it converges to a normal distribution with a smaller, compared to the conventional P\'olya random walk, diffusion coefficient. We formulate, as well, an auxiliary stochastic process whose distribution is identic to the distribution of the intermediate points $X^{(n)}_l$, $l < n$, which enables us to obtain the probability measure of different excursions and to define the asymptotic distribution of the number of "turns" of the PGRW trajectories.Comment: text shortened, new results added, appearing in J. Phys.