Mechanism and function of drosophila capa GPCR: a desiccation stress-responsive receptor with functional homology to human neuromedinU receptor

The capa peptide receptor, capaR (CG14575), is a G-protein coupled receptor (GPCR) for the D. melanogaster capa neuropeptides, Drm-capa-1 and -2 (capa-1 and -2). To date, the capa peptide family constitutes the only known nitridergic peptides in insects, so the mechanisms and physiological function of ligand-receptor signalling of this peptide family are of interest. Capa peptide induces calcium signaling via capaR with EC50 values for capa-1 = 3.06 nM and capa-2 = 4.32 nM. capaR undergoes rapid desensitization, with internalization via a b-arrestin-2 mediated mechanism but is rapidly re-sensitized in the absence of capa-1. Drosophila capa peptides have a C-terminal -FPRXamide motif and insect-PRXamide peptides are evolutionarily related to vertebrate peptide neuromedinU (NMU). Potential agonist effects of human NMU-25 and the insect -PRLamides [Drosophila pyrokinins Drm-PK-1 (capa-3), Drm-PK-2 and hugin-gamma [hugg]] against capaR were investigated. NMU-25, but not hugg nor Drm-PK-2, increases intracellular calcium ([Ca2+]i) levels via capaR. In vivo, NMU-25 increases [Ca2+]i and fluid transport by the Drosophila Malpighian (renal) tubule. Ectopic expression of human NMU receptor 2 in tubules of transgenic flies results in increased [Ca2+]i and fluid transport. Finally, anti-porcine NMU-8 staining of larval CNS shows that the most highly immunoreactive cells are capa-producing neurons. These structural and functional data suggest that vertebrate NMU is a putative functional homolog of Drm-capa-1 and -2. capaR is almost exclusively expressed in tubule principal cells; cell-specific targeted capaR RNAi significantly reduces capa-1 stimulated [Ca2+]i and fluid transport. Adult capaR RNAi transgenic flies also display resistance to desiccation. Thus, capaR acts in the key fluid-transporting tissue to regulate responses to desiccation stress in the fly

Magneto--Acoustic Energetics Study of the Seismically Active Flare of 15 February 2011

Multi--wavelength studies of energetic solar flares with seismic emissions have revealed interesting common features between them. We studied the first GOES X--class flare of the 24th solar cycle, as detected by the Solar Dynamics Observatory (SDO). For context, seismic activity from this flare (SOL2011-02-15T01:55-X2.2, in NOAA AR 11158) has been reported in the literature (Kosovichev, 2011; Zharkov et al., 2011). Based on Dopplergram data from the Helioseismic and Magnetic Imager (HMI), we applied standard methods of local helioseismology in order to identify the seismic sources in this event. RHESSI hard X-ray data are used to check the correlation between the location of the seismic sources and the particle precipitation sites in during the flare. Using HMI magnetogram data, the temporal profile of fluctuations in the photospheric line-of-sight magnetic field is used to estimate the magnetic field change in the region where the seismic signal was observed. This leads to an estimate of the work done by the Lorentz-force transient on the photosphere of the source region. In this instance this is found to be a significant fraction of the acoustic energy in the attendant seismic emission, suggesting that Lorentz forces can contribute significantly to the generation of sunquakes. However, there are regions in which the signature of the Lorentz-force is much stronger, but from which no significant acoustic emission emanates.Comment: Submitted to Solar Physic

Imaging Spectroscopy of a White-Light Solar Flare

We report observations of a white-light solar flare (SOL2010-06-12T00:57, M2.0) observed by the Helioseismic Magnetic Imager (HMI) on the Solar Dynamics Observatory (SDO) and the Reuven Ramaty High-Energy Solar Spectroscopic Imager (RHESSI). The HMI data give us the first space-based high-resolution imaging spectroscopy of a white-light flare, including continuum, Doppler, and magnetic signatures for the photospheric FeI line at 6173.34{\AA} and its neighboring continuum. In the impulsive phase of the flare, a bright white-light kernel appears in each of the two magnetic footpoints. When the flare occurred, the spectral coverage of the HMI filtergrams (six equidistant samples spanning \pm172m{\AA} around nominal line center) encompassed the line core and the blue continuum sufficiently far from the core to eliminate significant Doppler crosstalk in the latter, which is otherwise a possibility for the extreme conditions in a white-light flare. RHESSI obtained complete hard X-ray and \Upsilon-ray spectra (this was the first \Upsilon-ray flare of Cycle 24). The FeI line appears to be shifted to the blue during the flare but does not go into emission; the contrast is nearly constant across the line profile. We did not detect a seismic wave from this event. The HMI data suggest stepwise changes of the line-of-sight magnetic field in the white-light footpoints.Comment: 14 pages, 7 figures, Accepted by Solar Physic

Distribution modelling and statistical phylogeography: an integrative framework for generating and testing alternative biogeographical hypotheses

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73644/1/j.1365-2699.2007.01814.x.pd

A statistical correlation of sunquakes based on their seismic and white-light emission

Several mechanisms have been proposed to explain the transient seismic emission, i.e. “sunquakes,” from some solar flares. Some theories associate high-energy electrons and/or white-light emission with sunquakes. High-energy charged particles and their subsequent heating of the photosphere and/or chromosphere could induce acoustic waves in the solar interior. We carried out a correlative study of solar flares with emission in hard X-rays, enhanced continuum emission at 6173 Å, and transient seismic emission. We selected those flares observed by the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) with a considerable flux above 50 keV between 1 January 2010 and 26 June 2014. We then used data from the Helioseismic and Magnetic Imager onboard the Solar Dynamic Observatory to search for excess visible-continuum emission and new sunquakes not previously reported. We found a total of 18 sunquakes out of 75 flares investigated. All of the sunquakes were associated with an enhancement of the visible continuum during the flare. Finally, we calculated a coefficient of correlation for a set of dichotomic variables related to these observations. We found a strong correlation between two of the standard helioseismic detection techniques, and between sunquakes and visible-continuum enhancements. We discuss the phenomenological connectivity between these physical quantities and the observational difficulties of detecting seismic signals and excess continuum radiation

High-Energy Aspects of Solar Flares: Overview of the Volume

In this introductory chapter, we provide a brief summary of the successes and remaining challenges in understanding the solar flare phenomenon and its attendant implications for particle acceleration mechanisms in astrophysical plasmas. We also provide a brief overview of the contents of the other chapters in this volume, with particular reference to the well-observed flare of 2002 July 23Comment: This is the introductory article for a monograph on the physics of solar flares, inspired by RHESSI observations. The individual articles are to appear in Space Science Reviews (2011

Surface and capillary transitions in an associating binary mixture model

We investigate the phase diagram of a two-component associating fluid mixture in the presence of selectively adsorbing substrates. The mixture is characterized by a bulk phase diagram which displays peculiar features such as closed loops of immiscibility. The presence of the substrates may interfere the physical mechanism involved in the appearance of these phase diagrams, leading to an enhanced tendency to phase separate below the lower critical solution point. Three different cases are considered: a planar solid surface in contact with a bulk fluid, while the other two represent two models of porous systems, namely a slit and an array on infinitely long parallel cylinders. We confirm that surface transitions, as well as capillary transitions for a large area/volume ratio, are stabilized in the one-phase region. Applicability of our results to experiments reported in the literature is discussed.Comment: 12 two-column pages, 12 figures, accepted for publication in Physical Review E; corrected versio

Cosmological Imprints of Pre-Inflationary Particles

We study some of the cosmological imprints of pre-inflationary particles. We show that each such particle provides a seed for a spherically symmetric cosmic defect. The profile of this cosmic defect is fixed and its magnitude is linear in a single parameter that is determined by the mass of the pre-inflationary particle. We study the CMB and peculiar velocity imprints of this cosmic defect and suggest that it could explain some of the large scale cosmological anomalies.Comment: 31 pages, 7 figure

Svestka's Research: Then and Now

Zdenek Svestka's research work influenced many fields of solar physics, especially in the area of flare research. In this article I take five of the areas that particularly interested him and assess them in a "then and now" style. His insights in each case were quite sound, although of course in the modern era we have learned things that he could not readily have envisioned. His own views about his research life have been published recently in this journal, to which he contributed so much, and his memoir contains much additional scientific and personal information (Svestka, 2010).Comment: Invited review for "Solar and Stellar Flares," a conference in honour of Prof. Zden\v{e}k \v{S}vestka, Prague, June 23-27, 2014. This is a contribution to a Topical Issue in Solar Physics, based on the presentations at this meeting (Editors Lyndsay Fletcher and Petr Heinzel