What Water Works for SWOSU?

Bottled water has become a staple of our fast paced lives. Between 2000 to 2008, the sales of bottled water in the United States doubled from $6.1 to$12.6 billion (“Bottled Water,” 2008). One of the major reasons that bottled water drinkers give for their preference is convenience. Alternatively, critics assert that bottled water hurts the environment, is overpriced, and is no safer than tap water. This raises the question: does taste or other less physiological factors such as cost and availability affect what water people choose to drink? A study conducted at University of Applied Sciences Hamburg sampled females on whether type of water affecd sensitivity to taste (Hoehl, Schoenburger, & Busch- Stockfishch, 2010). Researchers found most subjects tasted a sourness when sampling water containing minerals. Nordin, Razani, Markison, & Murphy (2003) reported older subjects to have a poorer ability to discriminate between taste intensities. These researchers also observed some gender differences. Therefore, the objective of our study was to determine whether taste, age, gender, cost and/or availability have an effect on what water that people at Southwestern Oklahoma State University (SWOSU) actually prefer

Then and Now: Examining Memories of Pediatric Experiences and Their Influence on Opinions about Healthcare as an Adult

Children use their memories of healthcare experiences to navigate subsequent visits. The purpose of this exploratory study was to examine young adults’ (N = 343) memories of childhood medical experiences, how support from parents and the medical team influenced these memories, and how memories of pediatric experiences influence opinions about healthcare as an adult. The participants remembered having mild anxiety about childhood medical visits, feeling parents and the medical team were helpful with coping, and thinking the medical staff were supportive/friendly. Participants remembered having a relationship with their healthcare providers and the healthcare providers communicating with them as a child. The adults reported their current opinion and healthcare use was influenced by childhood healthcare experiences. These findings highlight the importance of parents and the medical team during pediatric healthcare visits. Also, visits during childhood were found to influence use of healthcare as an adult, highlighting the need for positive pediatric experiences, both at the doctor and dentist

Differential Downregulation of E-Cadherin and Desmoglein by Epidermal Growth Factor

Modulation of cell : cell junctions is a key event in cutaneous wound repair. In this study we report that activation of the epidermal growth factor (EGF) receptor disrupts cell : cell adhesion, but with different kinetics and fates for the desmosomal cadherin desmoglein and for E-cadherin. Downregulation of desmoglein preceded that of E-cadherin in vivo and in an EGF-stimulated in vitro wound reepithelialization model. Dual immunofluorescence staining revealed that neither E-cadherin nor desmoglein-2 internalized with the EGF receptor, or with one another. In response to EGF, desmoglein-2 entered a recycling compartment based on predominant colocalization with the recycling marker Rab11. In contrast, E-cadherin downregulation was accompanied by cleavage of the extracellular domain. A broad-spectrum matrix metalloproteinase inhibitor protected E-cadherin but not the desmosomal cadherin, desmoglein-2, from EGF-stimulated disruption. These findings demonstrate that although activation of the EGF receptor regulates adherens junction and desmosomal components, this stimulus downregulates associated cadherins through different mechanisms

Differential Downregulation of E-Cadherin and Desmoglein by Epidermal Growth Factor

Modulation of cell : cell junctions is a key event in cutaneous wound repair. In this study we report that activation of the epidermal growth factor (EGF) receptor disrupts cell : cell adhesion, but with different kinetics and fates for the desmosomal cadherin desmoglein and for E-cadherin. Downregulation of desmoglein preceded that of E-cadherin in vivo and in an EGF-stimulated in vitro wound reepithelialization model. Dual immunofluorescence staining revealed that neither E-cadherin nor desmoglein-2 internalized with the EGF receptor, or with one another. In response to EGF, desmoglein-2 entered a recycling compartment based on predominant colocalization with the recycling marker Rab11. In contrast, E-cadherin downregulation was accompanied by cleavage of the extracellular domain. A broad-spectrum matrix metalloproteinase inhibitor protected E-cadherin but not the desmosomal cadherin, desmoglein-2, from EGF-stimulated disruption. These findings demonstrate that although activation of the EGF receptor regulates adherens junction and desmosomal components, this stimulus downregulates associated cadherins through different mechanisms

Developing an obesity-cancer intervention for workplaces: Indigenous, Native American, Māori and other minority occupational settings

There is growing evidence for links between obesity and certain types of cancer. Studies done within Native American, Māori, and other Indigenous populations suggest the need to promote healthier lifestyles, including the maintenance of optimal body weight through nutrition and physical activity, to lower the risk factors of obesity-related cancers. What is missing is a program that combines culturally attuned workplace interventions that deal with obesity reduction as it relates to cancer prevention. The main purpose of this project was to discuss the process of developing an employee assistance program module to reduce the risk for obesity-related cancers. Expert curriculum developers specialising in workplace disease management assisted with the creation of a unique obesity and cancer prevention program. Several national leaders in Indigenous and minority health were consulted for feedback. The completed intervention included a six-session model with cultural features wrapped around topics of obesity-related cancer warning signs, diet and physical activity guidance, stress management, goal-setting, and resource linkage. A Native American workplace was selected for feasibility and pilot testing. Preliminary results are also discussed. Ultimately, this paper presents a novel intervention approach to address health issues for Native Americans, with indicators for use in other Indigenous populations globally

Compensation for Changing Motor Uncertainty

When movement outcome differs consistently from the intended movement, errors are used to correct subsequent movements (e.g., adaptation to displacing prisms or force fields) by updating an internal model of motor and/or sensory systems. Here, we examine changes to an internal model of the motor system under changes in the variance structure of movement errors lacking an overall bias. We introduced a horizontal visuomotor perturbation to change the statistical distribution of movement errors anisotropically, while monetary gains/losses were awarded based on movement outcomes. We derive predictions for simulated movement planners, each differing in its internal model of the motor system. We find that humans optimally respond to the overall change in error magnitude, but ignore the anisotropy of the error distribution. Through comparison with simulated movement planners, we found that aimpoints corresponded quantitatively to an ideal movement planner that updates a strictly isotropic (circular) internal model of the error distribution. Aimpoints were planned in a manner that ignored the direction-dependence of error magnitudes, despite the continuous availability of unambiguous information regarding the anisotropic distribution of actual motor errors

Floods, drying, habitat connectivity, and fish occupancy dynamics in restored and unrestored oxbows of West Central Iowa, USA

1. In the agricultural landscape of the Midwestern USA, waterways are highly altered. Oxbows are among the few remaining off‐channel habitats associated with streams, supporting fish assemblages that include the endangered Topeka shiners Notropis topeka in portions of their remaining range. Oxbow restorations seek to increase the number and quality of oxbows for Topeka shiners. For oxbows to provide optimal habitat, periods of isolation from streams and connection with streams during floods are necessary. 2. Water levels and patterns of drying and hydrological connectivity between 12 oxbows and their neighboring streams in West Central Iowa were continuously monitored from May to October 2011, and fish assemblages were assessed for responses to the differing hydrodynamics using dynamic occupancy modelling. 3. The 12 oxbows exhibited varied hydrodynamics and connectivity with streams. Two oxbows never contained fish; these oxbows never flooded and were among the three oxbows that were dry for the longest periods. 4. Occupancy modelling suggested that connection with the stream via floods significantly increased the probability of colonization, and low water level increased the probability of local extinction from oxbows. Thirteen of the 16 fish species encountered had detection probabilities over 60%, and eight had detection probabilities over 90%, including Topeka shiners. 5. None of the five previously restored oxbows flooded; all five contained fish, but only one contained Topeka shiners. Three of the four oxbows containing Topeka shiners flooded and all four dried at least once. 6. These results suggest that planning for future oxbow restorations should consider: (i) sites that flood frequently; and (ii) construction methods promoting alternating periods of isolation from and connection with streams

4th Biennial Employment Law Institute

Materials from the 4th Biennial Employment Law Institute held by UK/CLE in June 1994

Directing the evolution of Rubisco and Rubisco activase: first impressions of a new tool for photosynthesis research

During the last decade the practice of laboratory-directed protein evolution has become firmly established as a versatile tool in biochemical research by enabling molecular evolution toward desirable phenotypes or detection of novel structure–function interactions. Applications of this technique in the field of photosynthesis research are still in their infancy, but recently first steps have been reported in the directed evolution of the CO2-fixing enzyme Rubisco and its helper protein Rubisco activase. Here we summarize directed protein evolution strategies and review the progressive advances that have been made to develop and apply suitable selection systems for screening mutant forms of these enzymes that improve the fitness of the host organism. The goal of increasing photosynthetic efficiency of plants by improving the kinetics of Rubisco has been a long-term goal scoring modest successes. We discuss how directed evolution methodologies may one day be able to circumvent the problems encountered during this venture