Phage combination alleviates bacterial leaf blight of rice (Oryza sativa L.)

Rice bacterial leaf blight (BLB) is the most destructive bacterial diseases caused by Xanthomonas oryzae pv. oryzae (Xoo). Phages have been proposed as a green and efficient strategy to kill bacterial pathogens in crops, however, the mechanism of action of phages in the control of phyllosphere bacterial diseases remain unclear. Here, the glasshouse pot experiment results showed that phage combination could reduce the disease index by up to 64.3%. High-throughput sequencing technology was used to analyze the characteristics of phyllosphere microbiome changes and the results showed that phage combinations restored the impact of pathogen invasion on phyllosphere communities to a certain extent, and increased the diversity of bacterial communities. In addition, the phage combination reduced the relative abundance of epiphytic and endophytic Xoo by 58.9% and 33.9%, respectively. In particular, Sphingomonas and Stenotrophomonas were more abundant. According to structural equation modeling, phage combination directly and indirectly affected the disease index by affecting pathogen Xoo biomass and phage resistance. In summary, phage combination could better decrease the disease index. These findings provide new insights into phage biological control of phyllosphere bacterial diseases, theoretical data support, and new ideas for agricultural green prevention and control of phyllosphere diseases

Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi

Fusarium species are important seedborne pathogens that cause rice bakanae disease (RBD). In this study, 421 strains were isolated from 25 rice samples collected from Zhejiang, Anhui, and Jiangxi provinces of China. Furthermore, 407 isolates were identified as F. fujikuroi (80.05% isolation frequency), F. proliferatum (8.31%), F. equiseti (5.94%), F. incarnatum (2.61%), F. andiyazi (0.95%), and F. asiaticum (0.48%) based on morphology and translation elongation factor 1-alpha (TEF1-α) gene. Phylogenetic analysis of combined sequences of the RNA polymerase II largest subunit (RPB1), RNA polymerase II second largest subunit (RPB2), TEF1-α gene, and ribosomal DNA (rDNA) internal transcribed spacer (ITS) showed that 17 representative strains were attributed to six species. Pathogenicity tests showed that representative isolates possessed varying ability to cause symptoms of bakanae on rice seedlings. Moreover, the seed germination assay revealed that six isolates had different effects, such as inhibition of seed germination, as well as seed and bud rot. The loop mediated isothermal amplification (LAMP)-based assay were developed for the detection of F. fujikuroi. According to sequences of desaturase-coding gene promoter, a species-specific marker desM231 was developed for the detection of F. fujikuroi. The LAMP assay using seeds collected from field was validated, and diagnostics developed are efficient, rapid, and sensitive

Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial Community

Ralstonia Solanacearum is one of the most infectious soil-borne bacterial plant pathogens, causing tomato bacterial wilt (TBW). Nanotechnology is an emerging area of research, particularly the application of nanoparticles (NPs) as nanopesticides to manage plant disease is gaining attention nowadays. However, the interaction between NPs and rhizosphere bacterial communities remains largely elusive. This study indicated that metal NPs (CuO, ZnO, and FeO) reduced the incidence of bacterial wilt to varying degrees and affected the composition and structure of the rhizosphere bacterial community. The results revealed that the application of metal oxide NPs can improve the morphological and physiological parameters of TBW infected tomato plants. Among all, CuONPs amendments significantly increase the Chao1 and Shannon index. In the early stage (the second week), it significantly reduces the relative abundance of pathogens. However, the relative abundance of beneficial Streptomyces bacteria increased significantly, negatively correlated with the relative abundance of pathogenic bacteria. In addition, the nano-treatment group will enrich some potential beneficial bacteria such as species from Sphingomonadaceae, Rhizobiaceae, etc. In general, our research provides evidence and strategies for preventing and controlling soil-borne disease tomato bacterial wilt with metal oxide NPs

An X-Ray-dim “Isolated” Neutron Star in a Binary?

We report the discovery of a dark companion to 2MASS J15274848+3536572 with an orbital period of 6.14 hr. Combining the radial velocity from LAMOST observations and modeling of the multiband light curve, one obtains a mass function of ≃0.131 M _⊙ , an inclination of 45\buildrel{\circ}\over{.} {20}_{-0\buildrel{\circ}\over{.} \,20}^{+0\buildrel{\circ}\over{.} \,13} , and a mass ratio of ${0.631}_{-0.003}^{+0.014}$ , which demonstrate the binary nature of the dark companion with a mass of 0.98 ± 0.03 M _⊙ and a main-sequence K9-M0 star of 0.62 ± 0.01 M _⊙ . LAMOST optical spectra at a range of orbital phases reveal extra-peaked H _α emission that suggests the presence of an accretion disk. The dark companion does not seem to be a white dwarf because of the lack of any observed dwarf nova outbursts in the long-term data archive, although a magnetic white dwarf cannot be excluded. Alternatively, we propose a scenario wherein the dark companion is a neutron star, but we have not detected radio pulsations or a single pulse from the system with the FAST (Five-hundred-meter Aperture Spherical radio Telescope), which hints at a radio-quiet compact object. If the dark companion is identified as a neutron star, it will be the nearest (∼118 pc) and lightest neutron star. Furthermore, kinematic analysis of the system’s orbit in the galaxy may suggest its supernova event is associated with the radionuclide ^60 Fe signal observed from deep-sea crusts. This radio-quiet and X-ray-dim nearby neutron star may resemble an XDINS (X-ray-dim isolated neutron star) but in a binary

The Gastrodia elata genome provides insights into plant adaptation to heterotrophy

Gastrodia elata is an obligate mycoheterotrophic plant with highly reduced leaves and bracts in scape. Here, Yuan et al sequence and analyze its 1.06 Gb genome which provides insights in adaptation to a lifestyle of heterotrophy