Impact of Irrigation on Midsummer Surface Fluxes and Temperature under Dry Synoptic Conditions: A Regional Atmospheric Model Study of the U.S. High Plains

The impact of irrigation on the surface energy budget in the U.S. high plains is investigated. Four 15-day simulations were conducted: one using a 1997 satellite-derived estimate of farmland acreage under irrigation in Nebraska (control run), two using the Olson Global Ecosystem (OGE) vegetation dataset (OGE wet run and OGE dry run), and the fourth with the Kuchler vegetation dataset (natural vegetation run) as lower boundary conditions in the Colorado State University Regional Atmospheric Modeling System (RAMS). In the control and OGE wet simulations, the topsoil in the irrigated locations, up to a depth of 0.2 m, was saturated at 0000 UTC each day for the duration of the experiment (1–15 July 1997). In the other two runs, the soil was allowed to dry out, except when replenished naturally by rainfall. Identical observed atmospheric conditions were used along the lateral boundary in all four cases. The area-averaged model-derived quantities for the grid centered over Nebraska indicate significant differences in the surface energy fluxes between the control (irrigated) and the ‘‘dry’’ simulations. For example, a 36% increase in the surface latent heat flux and a 2.68C elevation in dewpoint temperature between the control run and the OGE dry run is shown. Surface sensible heat flux of the control run was 15% less and the near-ground temperature was 1.28C less compared to the OGE dry run. The differences between the control run and the natural vegetation run were similar but amplified compared to the control run–OGE dry run comparisons. Results of statistical analyses of long-term(1921–2000) surface temperature data from two sites representing locations of extensive irrigated and nonirrigated land uses appear to support model results presented herein of an irrigationrelated cooling in surface temperature. Growing season monthly mean and monthly mean maximum temperature data for the irrigated site indicate a steady decreasing trend in contrast to an increasing trend at the nonirrigated site

Impact of Irrigation on Midsummer Surface Fluxes and Temperature under Dry Synoptic Conditions: A Regional Atmospheric Model Study of the U.S. High Plains

The impact of irrigation on the surface energy budget in the U.S. high plains is investigated. Four 15-day simulations were conducted: one using a 1997 satellite-derived estimate of farmland acreage under irrigation in Nebraska (control run), two using the Olson Global Ecosystem (OGE) vegetation dataset (OGE wet run and OGE dry run), and the fourth with the Kuchler vegetation dataset (natural vegetation run) as lower boundary conditions in the Colorado State University Regional Atmospheric Modeling System (RAMS). In the control and OGE wet simulations, the topsoil in the irrigated locations, up to a depth of 0.2 m, was saturated at 0000 UTC each day for the duration of the experiment (1–15 July 1997). In the other two runs, the soil was allowed to dry out, except when replenished naturally by rainfall. Identical observed atmospheric conditions were used along the lateral boundary in all four cases. The area-averaged model-derived quantities for the grid centered over Nebraska indicate significant differences in the surface energy fluxes between the control (irrigated) and the ‘‘dry’’ simulations. For example, a 36% increase in the surface latent heat flux and a 2.68C elevation in dewpoint temperature between the control run and the OGE dry run is shown. Surface sensible heat flux of the control run was 15% less and the near-ground temperature was 1.28C less compared to the OGE dry run. The differences between the control run and the natural vegetation run were similar but amplified compared to the control run–OGE dry run comparisons. Results of statistical analyses of long-term(1921–2000) surface temperature data from two sites representing locations of extensive irrigated and nonirrigated land uses appear to support model results presented herein of an irrigationrelated cooling in surface temperature. Growing season monthly mean and monthly mean maximum temperature data for the irrigated site indicate a steady decreasing trend in contrast to an increasing trend at the nonirrigated site

Supersymmetric Chern-Simons Theories with Vector Matter

In this paper we discuss SU(N) Chern-Simons theories at level k with both fermionic and bosonic vector matter. In particular we present an exact calculation of the free energy of the N=2 supersymmetric model (with one chiral field) for all values of the 't Hooft coupling in the large N limit. This is done by using a generalization of the standard Hubbard-Stratanovich method because the SUSY model contains higher order polynomial interactions.Comment: 46 pages, 24 figures, v2: comments and references added, v3: a footnote in Section 3.5 adde

Oral Bisphosphonates and Risk of Atrial Fibrillation and Flutter in Women: A Self-Controlled Case-Series Safety Analysis

Background: A recent trial unexpectedly reported that atrial fibrillation, when defined as serious, occurred more often in participants randomized to an annual infusion of the relatively new parenteral bisphosphonate, zoledronic acid, than among those given placebo, but had limited power. Two subsequent population-based case-control studies of patients receiving a more established oral bisphosphonate, alendronic acid, reported conflicting results, possibly due to uncontrolled confounding factors.Methodology/Principal Findings: We used the United Kingdom General Practice Research Database to assess the risk of atrial fibrillation and flutter in women exposed to the oral bisphosphonates, alendronic acid and risedronate sodium. The self-controlled case-series method was used to minimise the potential for confounding. The age-adjusted incidence rate ratio for atrial fibrillation or flutter in individuals during their exposure to these oral bisphosphonates (n = 2195) was 1.07 (95% CI 0.94 - 1.21). The age-adjusted incidence rate ratio for alendronic acid (n = 1489) and risedronate sodium (n = 649) exposed individuals were 1.09 (95% CI 0.93 - 1.26) and 0.99 (95% CI 0.78 - 1.26) respectively. In post-hoc analyses, an increased risk of incident atrial fibrillation or flutter was detected for patients during their first few months of alendronic acid therapy.Conclusions/Significance: We found no robust evidence of an overall long-term increased risk of atrial fibrillation or flutter associated with continued exposure to the oral bisphosphonates, alendronic acid and risedronate sodium. A possible signal for an increase in risk during the first few months of therapy with alendronic acid needs to be re-assessed in additional studies

Vaccination with DNA plasmids expressing Gn coupled to C3d or alphavirus replicons expressing Gn protects mice against rift valley fever virus

Background: Rift Valley fever (RVF) is an arthropod-borne viral zoonosis. Rift Valley fever virus (RVFV) is an important biological threat with the potential to spread to new susceptible areas. In addition, it is a potential biowarfare agent. Methodology/Principal Findings: We developed two potential vaccines, DNA plasmids and alphavirus replicons, expressing the Gn glycoprotein of RVFV alone or fused to three copies of complement protein, C3d. Each vaccine was administered to mice in an all DNA, all replicon, or a DNA prime/replicon boost strategy and both the humoral and cellular responses were assessed. DNA plasmids expressing Gn-C3d and alphavirus replicons expressing Gn elicited high titer neutralizing antibodies that were similar to titers elicited by the live-attenuated MP12 virus. Mice vaccinated with an inactivated form of MP12 did elicit high titer antibodies, but these antibodies were unable to neutralize RVFV infection. However, only vaccine strategies incorporating alphavirus replicons elicited cellular responses to Gn. Both vaccines strategies completely prevented weight loss and morbidity and protected against lethal RVFV challenge. Passive transfer of antisera from vaccinated mice into naïve mice showed that both DNA plasmids expressing Gn-C3d and alphavirus replicons expressing Gn elicited antibodies that protected mice as well as sera from mice immunized with MP12. Conclusion/Significance: These results show that both DNA plasmids expressing Gn-C3d and alphavirus replicons expressing Gn administered alone or in a DNA prime/replicon boost strategy are effective RVFV vaccines. These vaccine strategies provide safer alternatives to using live-attenuated RVFV vaccines for human use. © 2010 Bhardwaj et al

Microtubules gate tau condensation to spatially regulate microtubule functions.

Tau is an abundant microtubule-associated protein in neurons. Tau aggregation into insoluble fibrils is a hallmark of Alzheimer's disease and other types of dementia1, yet the physiological state of tau molecules within cells remains unclear. Using single-molecule imaging, we directly observe that the microtubule lattice regulates reversible tau self-association, leading to localized, dynamic condensation of tau molecules on the microtubule surface. Tau condensates form selectively permissible barriers, spatially regulating the activity of microtubule-severing enzymes and the movement of molecular motors through their boundaries. We propose that reversible self-association of tau molecules, gated by the microtubule lattice, is an important mechanism of the biological functions of tau, and that oligomerization of tau is a common property shared between the physiological and disease-associated forms of the molecule

Unresolved issues with the assessment of multidecadal global land surface temperature trends

This paper documents various unresolved issues in using surface temperature trends as a metric for assessing global and regional climate change. A series of examples ranging from errors caused by temperature measurements at a monitoring station to the undocumented biases in the regionally and globally averaged time series are provided. The issues are poorly understood or documented and relate to micrometeorological impacts due to warm bias in nighttime minimum temperatures, poor siting of the instrumentation, effect of winds as well as surface atmospheric water vapor content on temperature trends, the quantification of uncertainties in the homogenization of surface temperature data, and the influence of land use/land cover (LULC) change on surface temperature trends. Because of the issues presented in this paper related to the analysis of multidecadal surface temperature we recommend that greater, more complete documentation and quantification of these issues be required for all observation stations that are intended to be used in such assessments. This is necessary for confidence in the actual observations of surface temperature variability and long-term trends

Effects of Clinically Relevant MPL Mutations in the Transmembrane Domain Revealed at the Atomic Level through Computational Modeling

BACKGROUND: Mutations in the thrombopoietin receptor (MPL) may activate relevant pathways and lead to chronic myeloproliferative neoplasms (MPNs). The mechanisms of MPL activation remain elusive because of a lack of experimental structures. Modern computational biology techniques were utilized to explore the mechanisms of MPL protein activation due to various mutations. RESULTS: Transmembrane (TM) domain predictions, homology modeling, ab initio protein structure prediction, and molecular dynamics (MD) simulations were used to build structural dynamic models of wild-type and four clinically observed mutants of MPL. The simulation results suggest that S505 and W515 are important in keeping the TM domain in its correct position within the membrane. Mutations at either of these two positions cause movement of the TM domain, altering the conformation of the nearby intracellular domain in unexpected ways, and may cause the unwanted constitutive activation of MPL's kinase partner, JAK2. CONCLUSIONS: Our findings represent the first full-scale molecular dynamics simulations of the wild-type and clinically observed mutants of the MPL protein, a critical element of the MPL-JAK2-STAT signaling pathway. In contrast to usual explanations for the activation mechanism that are based on the relative translational movement between rigid domains of MPL, our results suggest that mutations within the TM region could result in conformational changes including tilt and rotation (azimuthal) angles along the membrane axis. Such changes may significantly alter the conformation of the adjacent and intrinsically flexible intracellular domain. Hence, caution should be exercised when interpreting experimental evidence based on rigid models of cytokine receptors or similar systems

Mislocalization of Visual Stimuli: Independent Effects of Static and Dynamic Attention

Shifts of visual attention cause systematic distortions of the perceived locations of visual objects around the focus of attention. In the attention repulsion effect, the perceived location of a visual target is shifted away from an attention-attracting cue when the cue is presented before the target. Recently it has been found that, if the visual cue is presented after the target, the perceived location of the target shifts toward the location of the following cue. One unanswered question is whether a single mechanism underlies both attentional repulsion and attraction effects. We presented participants with two disks at diagonal locations as visual cues and two vertical lines as targets. Participants were asked to perform a forced-choice task to judge targets' positions. The present study examined whether the magnitude of the repulsion effect and the attraction effect would differ (Experiment 1), whether the two effects would interact (Experiment 2), and whether the location or the dynamic shift of attentional focus would determine the distortions effects (Experiment 3). The results showed that the effect size of the attraction effect was slightly larger than the repulsion effect and the preceding and following cues have independent influences on the perceived positions. The repulsion effect was caused by the location of attnetion and the attraction effect was due to the dynamic shift of attentional focus, suggesting that the underlying mechanisms for the retrospective attraction effect might be different from those for the repulsion effect