Regulation of axon repulsion by MAX-1 SUMOylation and AP-3.

During neural development, growing axons express specific surface receptors in response to various environmental guidance cues. These axon guidance receptors are regulated through intracellular trafficking and degradation to enable navigating axons to reach their targets. In Caenorhabditis elegans, the UNC-5 receptor is necessary for dorsal migration of developing motor axons. We previously found that MAX-1 is required for UNC-5-mediated axon repulsion, but its mechanism of action remained unclear. Here, we demonstrate that UNC-5-mediated axon repulsion in C. elegans motor axons requires both max-1 SUMOylation and the AP-3 complex β subunit gene, apb-3 Genetic interaction studies show that max-1 is SUMOylated by gei-17/PIAS1 and acts upstream of apb-3 Biochemical analysis suggests that constitutive interaction of MAX-1 and UNC-5 receptor is weakened by MAX-1 SUMOylation and by the presence of APB-3, a competitive interactor with UNC-5. Overexpression of APB-3 reroutes the trafficking of UNC-5 receptor into the lysosome for protein degradation. In vivo fluorescence recovery after photobleaching experiments shows that MAX-1 SUMOylation and APB-3 are required for proper trafficking of UNC-5 receptor in the axon. Our results demonstrate that SUMOylation of MAX-1 plays an important role in regulating AP-3-mediated trafficking and degradation of UNC-5 receptors during axon guidance

A Practical Case Study of the Interactive TV Service as a Time-Critical Product

In this paper, we conducted a case study of time-critical goods - NG goods. We expected the study to integrate the field of information management and the TV broadcasting field, thereby creating a new wave of potential for the information management field after e-commerce. We suggest two perspectives germane to industry development: the development of the whole industry, and, the operation of the individual companie

A Hybrid Meta-heuristic for the Container Loading Problem

It is very common in an enterprise daily operation to solve Container Loading Problem (CLP). Especially, it is an important issue in the logistic management. The problem aims to determine the arrangement of objects with the best utilization ratio in a container. It belongs to the combinatorial optimization problem. In this paper, a two-phased method focusing on the improvement of the efficiency and on the reducing of the problem size is proposed. In the first phase, a constructive method incorporated with a decision rule borrowing from ant colony optimization is used to construct tower set. The pheromone updating mechanism is useful in choosing proper object while constructing tower using decision rule. In the second phase, an improvement method based on genetic algorithm is used. First, the method sorts the towers by the utilization ratio and then assigns a number to each tower accordingly. The chromosome is a sequence of tower numbers which represents the arrangement of towers in the container’s bottom plane. The fitness function is defined as the utilization ratio. A new structure to store the pheromone is proposed which can help the ant in choosing the appropriate object while constructing tower. In this way, the efficiency of the method and the utilization of the container are improved

THE ANTECEDENTS OF AN INDIVIDUAL\u27S COMMITMENTS TOWARD CONTINUOUSLY USING SOCIAL NETWORK SITE

The Social network sites (SNS) has been rapid diffusion around the world. With the increasing importance of SNS, continuance intention also becomes a popular issue in the SNS context. SNS providers have to maintain better relationships with users and make individuals continue to use their sites. Based on this phenomenon, the objective of this study is to gain a better understanding of the continuance intention of SNS through examining the effects of commitments. Specifically, followed Meyer and Allen’s three-component model of commitment, we develop a theoretical model to understand the factors that influence normative, affective and continued commitment and investigate the effects of commitments on continuance intention in the SNS context. Through a survey-based empirical investigation, we anticipate the results to enhance our existing knowledge on continuance intention in the SNS context

Quantum mechanical Gaussian wavepackets of single relativistic particles

We study the evolutions of selected quasi-(1+1) dimensional wavepacket solutions to the Klein-Gordon equation for a relativistic charged particle in uniform motion or accelerated by a uniform electric field in Minkowski space. We explore how good the charge density of a Klein-Gordon wavepacket can be approximated by a Gaussian state with the single-particle interpretation. We find that the minimal initial width of a wavepacket for a good Gaussian approximation in position space is about the Compton wavelength of the particle divided by its Lorentz factor at the initial moment. Relativistic length contraction also manifests in the spreading of the wavepacket's charge density.Comment: 15 pages, 8 figure