Differential response to climate change among populations for woody plant species : an ecological and physiological approach

Changes in key climatic variables (e.g., atmospheric CO2, air temperature and water availability) are occurring at unprecedented rates and having substantial impacts on functionality, biodiversity and productivity of terrestrial ecosystems. Because forests dominate terrestrial net primary production and play a prominent role in the global carbon cycle, understanding the capacity of woody species to cope with simultaneously changing climatic variables is critical for the management of natural resources and the conservation of biodiversity. One fundamental way that plants may respond to rapid climate change in the short-term is to adjust their growth and physiology via phenotypic plasticity – the ability of a genotype to express multiple phenotypes in response to environmental change, which is thought to be particularly important for woody species with long generation times. For any given species, plant populations originating from different environments usually differ in their responses to the same environmental change, as evidence of intraspecific variation in phenotypic plasticity. Although some progress has been made on intraspecific variation in woody plant response to climate change, no studies have looked into the interactive effects of concurrently changing climatic variables on their intraspecific variation in phenotypic plasticity. Therefore, my PhD thesis was designed to assess the impacts of key climatic variables (i.e., [CO2], temperature, and water availability) on growth and physiology of woody plant populations originating from contrasting environments, with a focus on the intraspecific variation in their capacity to cope with climate change. Three Australian native woody species representing different taxa and functional groups were included in this research: Telopea speciosissima (Proteaceae; Shrub; open xvi woodland), Eucalyptus grandis (Myrtaceae; Tree; wet forest) and Eucalyptus tereticornis (Myrtaceae; Tree; dry forest), each of which consisted of two populations originating from climatically differentiated regions. Treatment levels (i.e., changes in [CO2], temperature, and water availability) in this research were chosen based on predicted climatic conditions within this century. My goal was to use these woody species to generate improve understanding of woody plant growth and physiological responses under future climatic scenarios. my PhD research addressed the main and interactive effects of changes in multiple climatic variables (i.e., [CO2], temperature, and water availability) on growth and physiology of three woody species representing different taxa and functional groups, with a focus on the intraspecific variation in their responses between populations originating from different environments. Results of this research were reported based on the treatment levels chosen for the experiments. Significant intraspecific variation in growth plasticity when responding to a constant mild warming (TE; ambient + 3.5–4.0 °C) was found in all three species, and intraspecific variation in photosynthetic responses to a short-term heat stress (ambient + 8 °C) was observed in the two Eucalyptus species. In contrast, populations did not differ in their growth or photosynthetic responses to elevated [CO2] (CE) or to sustained drought in most cases for all three species. These results together suggest that temperature would be more effective than [CO2] or water availability in exposing intraspecific variation in phenotypic plasticity for woody plant populations under future climates. The relationships between phenotypic plasticity and source environment variability of plant populations differed among the three species. Results from the two Eucalyptus species confirmed the general prediction that greater levels of environmental variability will select for plants with greater phenotypic plasticity, while findings from T. speciosissima contradicted the paradigm, indicating that woody plant populations originating from more variable environments may not necessarily show greater phenotypic plasticity in response to climate change. In addition, TE negatively affected plant resistance to drought and heat stress exacerbated the negative effects of drought on plant responses, suggesting that temperature may influence the responses of woody plants to drought under future climates. Overall, my PhD work expands current knowledge regarding the interactive effects of simultaneously changing climatic variables on woody plant growth and physiology. More importantly, this research contributes valuable information on intraspecific variation in phenotypic plasticity of woody plant populations in response to changing climatic variables, as well as the association between phenotypic plasticity and source environment variability, which will assist in making robust predictions of the distribution and abundance of woody species under future climates

Constant-size ciphertexts in threshold attribute-based encryption without dummy attributes

Attribute-based encryption (ABE) is an augmentation of public key encryption that allows users to encrypt and decrypt messages based on users’ attributes. In a ( t, s ) threshold ABE, users who can decrypt a ciphertext must hold at least t attributes among the s attributes specified by the encryptor. At PKC 2010, Herranz, Laguillaumie and Ràfols proposed the first threshold ABE with constant-size ciphertexts. In order to ensure the encryptor can flexibly select the attribute set and a threshold value, they use dummy attributes to satisfy the decryption requirement. The advantage of their scheme is that any addition or removal of the attributes will not require any change to users’ private keys or public parameters. Unfortunately, the need for dummy attributes makes their scheme inefficient, since the computational cost of encryption is linear to the size of selected attribute set and dummy attribute set. In this work, we improve Herranz et al.’s work, and propose a new threshold ABE scheme which does not use any dummy attribute . Our scheme not only retains the nice feature of Herranz et al.’s scheme, but also offers two improvements in comparison to the previous work. Firstly, the computational costs of encryption and decryption are only linear in the size of the selected attribute set. Secondly, without any dummy attribute, most of the computations can be conducted without the knowledge of the threshold t . Hence, threshold change in the encryption phase does not require complete recomputation of the ciphertext

The impacts of Ramadan fasting for patients with non-alcoholic fatty liver disease (NAFLD): a systematic review

BackgroundNumerous studies have explored the impacts of Ramadan fasting on Non-alcoholic fatty liver disease (NAFLD). Therefore, the objective of this systematic review was to analyze and summarize all clinical studies regarding the impacts of Ramadan fasting for patients with NAFLD.MethodsWe performed a comprehensive search of the Embase, Cochrane, and PubMed databases from inception to September 1, 2023. All clinical studies concerning the impacts of Ramadan fasting on patients with NAFLD were included.ResultsIn total, six studies with 397 NAFLD patients comprising five prospective studies and one retrospective study were included in the systematic review. All six studies were assessed as high-quality. Ramadan fasting may offer potential benefits for patients with NAFLD, including improvements in body weight, body composition, cardiometabolic risk factors, glucose profiles, liver parameters, and inflammation markers.ConclusionRamadan fasting might be an effective dietary intervention for NAFLD. However, the number of studies examining the impacts of Ramadan fasting for patients with NAFLD is relatively limited. Therefore, more high-quality research is needed to further our understanding of the benefits of Ramadan fasting for NAFLD.Systematic review registrationhttps://inplasy.com, identifier 202390102

Ciphertext-Policy Attribute-Based Encrypted Data Equality Test and Classification

Thanks to the ease of access and low expenses, it is now popular for people to store data in cloud servers. To protect sensitive data from being leaked to the outside, people usually encrypt the data in the cloud. However, management of these encrypted data becomes a challenging problem, e.g. data classification. Besides, how to selectively share data with other users is also an important and interesting problem in cloud storage. In this paper, we focus on ciphertext-policy attribute based encryption with equality test (CP-ABEET). People can use CP-ABEET to implement not only flexible authorization for the access to encrypted data, but also efficient data label classification, i.e. test of whether two encrypted data contain the same message. We construct an efficient CP-ABEET scheme, and prove its security based on a reasonable number-theoretic assumption. Compared with the only existing CP-ABEET scheme, our construction is more efficient in key generation, and has shorter attribute-related secret keys and better security

Limited hydraulic recovery in seedlings of six tree species with contrasting leaf habits in subtropical China

Subtropical tree species may experience severe drought stress due to variable rainfall under future climates. However, the capacity to restore hydraulic function post-drought might differ among co-occurring species with contrasting leaf habits (e.g., evergreen and deciduous) and have implications for future forest composition. Moreover, the links between hydraulic recovery and physiological and morphological traits related to water-carbon availability are still not well understood. Here, potted seedlings of six tree species (four evergreen and two deciduous) were grown outdoors under a rainout shelter. They grew under favorable water conditions until they were experimentally subjected to a soil water deficit leading to losses of ca. 50% of hydraulic conductivity, and then soils were re-watered to field capacity. Traits related to carbon and water relations were measured. There were differences in drought responses and recovery between species, but not as a function of evergreen or deciduous groups. Sapindus mukorossi exhibited the most rapid drought response, which was associated with a suite of physiological and morphological traits (larger plant size, the lowest hydraulic capacitance (Cbranch), higher minimum conductance (gmin) and lower HV (Huber value)). Upon re-watering, xylem water potential exhibited fast recovery in 1–3 days among species, while photosynthesis at saturating light (Asat) and stomatal conductance (gs) recovery lagged behind water potential recovery depending on species, with gs recovery being more delayed than Asat in most species. Furthermore, none of the six species exhibited significant hydraulic recovery during the 7 days re-watering period, indicating that xylem refilling was apparently limited; in addition, NSC availability had a minimal role in facilitating hydraulic recovery during this short-term period. Collectively, if water supply is limited by insignificant hydraulic recovery post-drought, the observed carbon assimilation recovery of seedlings may not be sustained over the longer term, potentially altering seedling regeneration and shifting forest species composition in subtropical China under climate change.This work was supported by grants from the National Natural Science Foundation of China (31600483 and 31760111) and the Natural Science Talent Funding of Guizhou University (202132)

KASP-IEva: an intelligent typing evaluation model for KASP primers

KASP marker technology has been used in molecular marker-assisted breeding because of its high efficiency and flexibility, and an intelligent evaluation model of KASP marker primer typing results is essential to improve the efficiency of marker development on a large scale. To this end, this paper proposes a gene population delineation method based on NTC identification module and data distribution judgment module to improve the accuracy of K-Means clustering, and introduces a decision tree to construct the KASP-IEva primer typing evaluation model. The model firstly designs the NTC identification module and data distribution judgment module to extract four types of data, grouping and categorizing to achieve the improvement of the distinguishability of amplification product signals; secondly, the K-Means algorithm is used to aggregate and classify the data, to visualize the five aggregated clusters and to obtain the morphology location eigenvalues; lastly, the evaluation criteria for the typing effect level are constructed, and the logical decision tree is used to make conditional discrimination on the eigenvalues in order to realize the score prediction. The performance of the model was tested by the KASP marker typing test results of 2519 groups of cotton varieties, and the following conclusions were obtained: the model is able to visualize the aggregation and classification effects of the amplification products of NTC, pure genotypes, heterozygous genotypes, and untyped genotypes, enabling rapid and accurate KASP marker typing evaluation. Comparing and analyzing the model evaluation results with the expert evaluation results, the average accuracy rate of the four grades evaluated by the model was 87%, and the overall evaluation results showed an uneven distribution of the grades with significant differential characteristics. When evaluating 2519 KASP fractal maps, the expert evaluation consumes 15 hours, and the model evaluation only uses 8min27.45s, which makes the model intelligent evaluation significantly better than the expert evaluation from the perspective of time. The establishment of the model will further enhance the application of KASP markers in molecular marker-assisted breeding and provide technical support for the large-scale screening and identification of excellent genotypes

The roles of divergent and parallel molecular evolution contributing to thermal adaptive strategies in trees

Local adaptation is a driver of biological diversity, and species may develop analogous (parallel evolution) or alternative (divergent evolution) solutions to similar ecological challenges. We expect these adaptive solutions would culminate in both phenotypic and genotypic signals. Using two Eucalyptus species (Eucalyptus grandis and Eucalyptus tereticornis) with overlapping distributions grown under contrasting ‘local’ temperature conditions to investigate the independent contribution of adaptation and plasticity at molecular, physiological and morphological levels. The link between gene expression and traits markedly differed between species. Divergent evolution was the dominant pattern driving adaptation (91% of all significant genes); but overlapping gene (homologous) responses were dependent on the determining factor (plastic, adaptive or genotype by environment interaction). Ninety-eight percent of the plastic homologs were similarly regulated, while 50% of the adaptive homologs and 100% of the interaction homologs were antagonistical. Parallel evolution for the adaptive effect in homologous genes was greater than expected but not in favour of divergent evolution. Heat shock proteins for E. grandis were almost entirely driven by adaptation, and plasticity in E. tereticornis. These results suggest divergent molecular evolutionary solutions dominated the adaptive mechanisms among species, even in similar ecological circumstances. Suggesting that tree species with overlapping distributions are unlikely to equally persist in the future

Effect of potassium simplex optimization medium (KSOM) and embryo screening on the production of human lactoferrin transgenic cloned dairy goats

In this study, we produced cloned transgenic dairy goat based on dairy goat ear skin fibroblast as donor cells for nuclear transfer (NT), which were modified by human lactoferrin (hLF) gene. The developmental competence of NT embryos was compared with either between different embryo culture medium, potassium simplex optimization medium (KSOM) and tissue culture medium (TCM 199), or different classification of NT embryos (48 h after fusion). First we cultured NT embryos to cleavage stage (48 h after fusion) by TCM 199 supplemented with 1 mg/ml bovine serum albumin BSA and KSOM, then used TCM 199 supplemented with 10% FBS to culture them to blastula stage. The results show that the NT embryos in KSOM (19.5%) were superior to TCM 199 (10.6%) in blastulation. In the second experiment, we found that the growth rate of NT embryos (48 h after fusion) was different, then we divided them into four groups: 2-cell, 3- to 4-cell, 5- to 8-cell and >8-cell in stereo microscope and cultured them in vitro respectively. The results show day-2 embryos at 3-4cell and 5-8cell stage (31.9 and 28.2%, P < 0.05) had higher blastocyst formation rates than those at both 2-cell (9.1%) and >8-cell (8.3%) stage, and finally three healthy cloned transgenic goat were successfully produced using 3-8 cell embryos at Day-2 (82%). Using Hoechst 33342 staining, we also found that the >8 cells embryos at Day- 2 demonstrated higher frequency of fragmentation, which may be the one cause of the low blastocyst formation rate. This study therefore demonstrates that KSOM medium could be selected as the early embryo culture medium, and 3-8 cell embryos at day-2 (48 h after fusion) may be the suitable embryos for transplantation, which could reduce the nuclei fragmentation and result in good quality blastocysts that may also enhance the efficiency of transgenic cloned dairy goats production, as well as decrease the economic loss due to embryonic mortality when embryos are transferred to synchronized recipients.Key words: Nuclear transfer, KSOM, transgenic, human lactoferrin, dairy goat

The effect of Er3+ concentration on the kinetics of multiband upconversion in NaYF4:Yb/Er microcrystals

In Yb-Er co-doped upconversion (UC) nanomaterials, upconversion luminescence (UCL) can be modulated to generate multiband UCL emissions by changing the concentration of activator Er3+. Nonetheless, the effect of the Er3+ concentrations on the kinetics of these emissions is still unknown. We here study the single β-NaYF4:Yb3+/Er3+ microcrystal (MC) doped with different Er3+ concentrations by nanosecond time-resolved spectroscopy. Interestingly, different Er3+ doping concentrations exhibit different UCL emission bands and UCL response rates. At low Er3+ doping concentrations (1 mol%), multiband emission in β-NaYF4:Yb3+/Er3+ (20/1 mol%) MCs could not be observed and the response rate of UCL was slow (5–10 μs) in β-NaYF4:Yb3+/Er3+. Increasing the Er3+ doping concentration to 10 mol% can shorten the distance between Yb3+ ions and Er3+ ions, which promotes the energy transfer between them. β-NaYF4:Yb3+/Er3+ (20/10 mol%) can achieve obvious multiband UCL and a quick response rate (0.3 µs). However, a further increase in the Er doping concentration (80 mol%) makes MCs limited by the CR process and cannot achieve the four-photon UC process (4F5/2 → 2K13/2 and 2H9/2 → 2D5/2). Therefore, the result shows that changing the Er3+ doping concentration could control the energy flow between the different energy levels in Er3+, which could affect the response time and UCL emission of the Yb/Er doped rare earth materials. Our work can facilitate the development of fast-response optoelectronics, optical-sensing, and display industries