Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection

IntroductionPeanut (Arachis hypogaea L.) is an important cash crop worldwide. Compared with the ordinary peanut with pure pink testa, peanut with variegated testa color has attractive appearance and a higher market value. In addition, the variegated testa represents a distinct regulation pattern of anthocyanin accumulation in integument cells.MethodsIn order to identify the genetic locus underlying variegated testa color in peanut, two populations were constructed from the crosses between Fuhua 8 (pure-pink testa) and Wucai (red on white variegated testa), Quanhonghua 1 (pure-red testa) and Wucai, respectively. Genetic analysis and bulked sergeant analysis sequencing were applied to detect and identify the genetic locus for variegated testa color. Marker-assisted selection was used to develop new variegated testa peanut lines.ResultsAs a result, all the seeds harvested from the F1 individuals of both populations showed the variegated testa type with white trace. Genetic analysis revealed that the pigmentation of colored region in red on white variegated testa was controlled by a previous reported gene AhRt1, while the formation of white region (un-pigmented region) in variegated testa was controlled by another single genetic locus. This locus, named as AhVt1 (Arachis hypogaea Variegated Testa 1), was preliminary mapped on chromosome 08 through bulked sergeant analysis sequencing. Using a secondary mapping population derived from the cross between Fuhua 8 and Wucai, AhVt1 was further mapped to a 1.89-Mb genomic interval by linkage analysis, and several potential genes associated with the uneven distribution of anthocyanin, such as MADS-box, MYB, and Chalcone synthase-like protein, were harbored in the region. Moreover, the molecular markers closely linked to the AhVt1 were developed, and the new variegated testa peanut lines were obtained with the help of marker-assisted selection.ConclusionOur findings will accelerate the breeding program for developing new peanut varieties with “colorful” testa colors and laid a foundation for map-based cloning of gene responsible for variegated testa

A Major and Stable QTL for Bacterial Wilt Resistance on Chromosome B02 Identified Using a High-Density SNP-Based Genetic Linkage Map in Cultivated Peanut Yuanza 9102 Derived Population

Bacterial wilt (BW) is one of the important diseases limiting the production of peanut (Arachis hypogaea L.) worldwide. The sufficient precise information on the quantitative trait loci (QTL) for BW resistance is essential for facilitating gene mining and applying in molecular breeding. Cultivar Yuanza 9102 is BW resistant, bred from wide cross between cultivated peanut Baisha 1016 and a wild diploid peanut species A. chacoense with BW resistance. In this study, we aim to map the major QTLs related to BW-resistance in Yuanza 9102. A high density SNP-based genetic linkage map was constructed through double-digest restriction-site-associated DNA sequencing (ddRADseq) technique based on Yuanza 9102 derived recombinant inbred lines (RILs) population. The map contained 2,187 SNP markers distributed on 20 linkage groups (LGs) spanning 1566.10 cM, and showed good synteny with AA genome from A. duranensis and BB genome from A. ipaensis. Phenotypic frequencies of BW resistance among RIL population showed two-peak distribution in four environments. Four QTLs explaining 5.49 to 23.22% phenotypic variance were identified to be all located on chromosome B02. The major QTL, qBWB02.1 (12.17–23.33% phenotypic variation explained), was detected in three environments showing consistent and stable expression. Furthermore, there was positive additive effect among these major and minor QTLs. The major QTL region was mapped to a region covering 2.3 Mb of the pseudomolecule B02 of A. ipaensis which resides in 21 nucleotide-binding site -leucine-rich repeat (NBS-LRR) encoding genes. The result of the major stable QTL (qBWB02.1) not only offers good foundation for discovery of BW resistant gene but also provide opportunity for deployment of the QTL in marker-assisted breeding in peanut

Gene expression and DNA methylation altering lead to the high oil content in wild allotetraploid peanut (A. monticola)

IntroductionThe wild allotetraploid peanut Arachis monticola contains a higher oil content than the cultivated allotetraploid Arachis hypogaea. Besides the fact that increasing oil content is the most important peanut breeding objective, a proper understanding of its molecular mechanism controlling oil accumulation is still lacking.MethodsWe investigated this aspect by performing comparative transcriptomics from developing seeds between three wild and five cultivated peanut varieties.ResultsThe analyses not only showed species-specific grouping transcriptional profiles but also detected two gene clusters with divergent expression patterns between two species enriched in lipid metabolism. Further analysis revealed that expression alteration of lipid metabolic genes with co-expressed transcription factors in wild peanut led to enhanced activity of oil biogenesis and retarded the rate of lipid degradation. In addition, bisulfite sequencing was conducted to characterize the variation of DNA methylation between wild allotetraploid (245, WH 10025) and cultivated allotetraploid (Z16, Zhh 7720) genotypes. CG and CHG context methylation was found to antagonistically correlate with gene expression during seed development. Differentially methylated region analysis and transgenic assay further illustrated that variations of DNA methylation between wild and cultivated peanuts could affect the oil content via altering the expression of peroxisomal acyl transporter protein (Araip.H6S1B).DiscussionFrom the results, we deduced that DNA methylation may negatively regulate lipid metabolic genes and transcription factors to subtly affect oil accumulation divergence between wild and cultivated peanuts. Our work provided the first glimpse on the regulatory mechanism of gene expression altering for oil accumulation in wild peanut and gene resources for future breeding applications

Quaternary vegetation history in Hungary

Detailed information of SSR markers used for genotyping the RIL population. (XLSX 49 kb

Modeling and Control of a Linear Piezoelectric Actuator

To improve the output displacement of piezoelectric actuators, a linear piezoelectric actuator based on a multistage amplifying mechanism with a small volume, large thrust, high resolution, high precision, and fast response speed is proposed. However, inherent nonlinear characteristics, such as hysteresis and creep, significantly affect the output accuracy of piezoelectric actuators and may cause system instability. Therefore, a complex nonlinear hysteresis mathematical model with a high degree of fit was established. A Play operator was introduced into the backpropagation neural network, and a genetic algorithm (GA) was used to reduce the probability of the fitting of the neural network model falling into a local minimum. Moreover, simulation and experimental test platforms were constructed. The results showed that the maximum displacement of the actuator was 558.3 μm under a driving voltage of 150 V and a driving frequency of 1 Hz. The complex GA-BP neural network model of the piezoelectric actuator not only exhibited high modeling accuracy but also solved the problems of strong randomness and slow convergence. Compared with other control algorithms, the GA-BP fuzzy PID control exhibited higher control precision

Macrophages in Lung Injury, Repair, and Fibrosis

Fibrosis progression in the lung commonly results in impaired functional gas exchange, respiratory failure, or even death. In addition to the aberrant activation and differentiation of lung fibroblasts, persistent alveolar injury and incomplete repair are the driving factors of lung fibrotic response. Macrophages are activated and polarized in response to lipopolysaccharide- or bleomycin-induced lung injury. The classically activated macrophage (M1) and alternatively activated macrophage (M2) have been extensively investigated in lung injury, repair, and fibrosis. In the present review, we summarized the current data on monocyte-derived macrophages that are recruited to the lung, as well as alveolar resident macrophages and their polarization, pyroptosis, and phagocytosis in acute lung injury (ALI). Additionally, we described how macrophages interact with lung epithelial cells during lung repair. Finally, we emphasized the role of macrophage polarization in the pulmonary fibrotic response, and elucidated the potential benefits of targeting macrophage in alleviating pulmonary fibrosis

Temporal and Spatial Variations in the Leaf Area Index and Its Response to Topography in the Three-River Source Region, China from 2000 to 2017

The Three-River Source Region (TRSR) is an important area for the ecological security of China. Vegetation growth has been affected by the climate change, topography, and human activities in this area. However, few studies have focused on analyzing time series tendencies of vegetation change in various terrain conditions. To address this issue in the TRSR, this study explored vegetation stability, tendency, and sustainability with multiple methods (e.g., coefficient of variation, Theil-Sen median trend analysis, Mann-Kendall test, and Hurst index) based on the 2000–2017 Global LAnd Surface Satellite Leaf Area Index (GLASS LAI) product. The differentiation patterns of LAI variations and multiyear mean LAI value under different topographic factors were also investigated in combination with digital elevation model (DEM). The results showed that (1) the mean LAI value in the study area increased, with a linear tendency of 0.013·10 a−1; (2) LAI values decreased from southeast to northwest in terms of spatial distribution and the CV indicated LAI variations were relatively stable; (3) the trend analysis revealed that the improved area of LAI accounted for 62.72% which was larger than the degraded area (37.28%), and hurst index revealed a weak anti-sustaining effect of the current tendencies; and (4) the increasing trend was found in multiyear mean LAI value as relief amplitude and slope increased, while LAI stability improved with increasing slope. They exhibited a clear regular pattern. Moreover, significant improvement in LAI generally occurred in low-altitude and flat areas. Finally, the overall improvement and sustainability of LAI improved when moving from sunny aspects to shady aspects, but the LAI stability decreased. Note that vegetation degradation was observed in some high slope areas and was further aggravated. This study is beneficial for revealing the spatial and temporal changes of LAI and their changing rules as a function of different topographic factors in the TRSR. Meanwhile, the results of this study provide theoretical support for sustainable development of this area