Phase Reversal Method for Damage Imaging in Composite Laminates Based on Data Fusion

This paper proposes a phase-reversal method (PRM) for damage imaging in plate structures. The PRM is a novel Lamb-wave-based method that mainly focuses on phase spectrum information of scattering waves reflected from a defect. The PRM reverses the phase angle along the propagation path by using the inverse Fourier transform first, and then the reversal reconstruction of the wave field in the frequency domain is performed for damage imaging. The proposed method analyzes the scattered wave field without using the baseline data and structural parameters. Moreover, dispersion characteristics and anisotropy are not involved in the process of damage positioning, thus making the PRM suitable for damage monitoring of composite laminates. To improve the PRM accuracy further, a combined addition and multiplication method of the correlation coefficient (CAMM) is proposed, which can reduce the effects of phase and noise artifacts and distortion. The results of the finite element simulations and experiments show that the combination of the PRM and CAMM methods can accurately locate damage in composite structures. Therefore, the PRM and CAMM methods have great application potential in damage imaging in composite laminates

Phase Reversal Method for Damage Imaging in Composite Laminates Based on Data Fusion

This paper proposes a phase-reversal method (PRM) for damage imaging in plate structures. The PRM is a novel Lamb-wave-based method that mainly focuses on phase spectrum information of scattering waves reflected from a defect. The PRM reverses the phase angle along the propagation path by using the inverse Fourier transform first, and then the reversal reconstruction of the wave field in the frequency domain is performed for damage imaging. The proposed method analyzes the scattered wave field without using the baseline data and structural parameters. Moreover, dispersion characteristics and anisotropy are not involved in the process of damage positioning, thus making the PRM suitable for damage monitoring of composite laminates. To improve the PRM accuracy further, a combined addition and multiplication method of the correlation coefficient (CAMM) is proposed, which can reduce the effects of phase and noise artifacts and distortion. The results of the finite element simulations and experiments show that the combination of the PRM and CAMM methods can accurately locate damage in composite structures. Therefore, the PRM and CAMM methods have great application potential in damage imaging in composite laminates

TCTP is a critical factor in shrimp immune response to virus infection.

The translationally controlled tumor protein (TCTP) is an abundant, ubiquitous, and conserved protein which plays important roles in a number of biological processes. In the present study, the TCTP in shrimp Litopenaeus vannamei was analyzed. The TCTP of L.vannamei, a 168-amino-acid polypeptide, shares a high degree of similarity with TCTPs from other species, having two TCTP protein signatures at the 45-55 aa and 123-145 aa motif. The mRNA and protein levels from different tissues were detected with the highest in muscle and the lowest in heart among all examined tissues. In addition, temporal TCTP expression was significantly up-regulated at 16 h and 48 h following infection with white spot syndrome virus (WSSV). Lastly, silencing of TCTP with dsRNA led to a significant increase of WSSV loads. These results provide new insights into the importance of TCTP as an evolutionarily conserved molecule for shrimp innate immunity against virus infection

Bioinformatic analyzes and validation of cystathionine gamma-lyase as a prognostic biomarker and related to immune infiltrates in hepatocellular carcinoma

Background: The role of cystathionine γ-lyase (CTH) in the prognosis and immune invasion of hepatocellular carcinoma (HCC) remains poorly understood. Methods: In this study, the clinical data of patients with HCC were analyzed, and the expression level of CTH was compared between HCC and normal tissues using the R package and various databases. Results: We found that CTH expression was significantly decreased in HCC compared with normal tissues, and its expression was associated with various clinicopathological factors, including tumor stage, gender, tumor status, residual tumor, histologic stage, race, alpha-fetoprotein (AFP), albumin, drinking, and smoking. Our results suggest that CTH might be a protective factor for the survival of patients with HCC. Further functional analysis revealed that high CTH expression was enriched in the Reactome signaling by interleukins and the Reactome neutrophil degranulation. Moreover, CTH expression was closely correlated with a variety of immune cells, including a negative correlation with the CD56 (bright) NK cells and follicular helper T cell (TFH), while a positive correlation with Th17 cells and central memory T cell (Tcm). High expression of CTH in immune cells predicted a better prognosis of HCC. Our findings further indicated Pyridoxal phosphate, l-cysteine, Carboxymethylthio-3-(3-chlorophenyl)-1,2,4-oxadiazol, 2-[(3-Hydroxy-2-Methyl-5-Phosphonooxymethyl-Pyridin-4-Ylmethyl)-Imino]-5-phosphono-pent-3-enoic acid and L-2-amino-3-butynoic acid as potential target candidate medications for HCC treatment based on CTH. Conclusion: Our study suggests that CTH can serve as a biomarker to predict the prognosis and immune infiltration of HCC

Tunable analog thermal material.

Naturally-occurring thermal materials usually possess specific thermal conductivity (κ), forming a digital set of κ values. Emerging thermal metamaterials have been deployed to realize effective thermal conductivities unattainable in natural materials. However, the effective thermal conductivities of such mixing-based thermal metamaterials are still in digital fashion, i.e., the effective conductivity remains discrete and static. Here, we report an analog thermal material whose effective conductivity can be in-situ tuned from near-zero to near-infinity κ. The proof-of-concept scheme consists of a spinning core made of uncured polydimethylsiloxane (PDMS) and fixed bilayer rings made of silicone grease and steel. Thanks to the spinning PDMS and its induced convective effects, we can mold the heat flow robustly with continuously changing and anisotropic κ. Our work enables a single functional thermal material to meet the challenging demands of flexible thermal manipulation. It also provides platforms to investigate heat transfer in systems with moving components

Nowhere to run: oligo (p-phenylene vinylene) kills oral intracellular bacteria photodynamically

Abstract Bacterial infections pose a severe threat to human health due to the exacerbation of antibiotic resistance and intracellular bacterial infections. Research suggests that oligo(p-phenylene vinylene) (OPV), commonly employed in the manufacture of organic solar batteries, can help address this issue. This study demonstrates the ability of OPV to target and sterilize intracellular Porphyromonas gingivalis and methicillin-resistant Staphylococcus aureus (MRSA) photodynamically. Most notably, OPV specifically targets bacteria without affecting healthy cells under dark conditions. Its chemical composition includes a conjugated backbone and ionic imidazole side chains, which allow OPV to bind to cell membranes. Furthermore, dental blue light curing lamps may excite OPV. Compared with antibiotics and traditional photosensitizers, OPV proves to be a potentially superior solution to eradicate intracellular microbial infections, both in fundamental research and clinical applications

Robustly printable freeform thermal metamaterials

10.1038/s41467-021-27543-7Nature Communications121722

Expression profiles of <i>L. Vannamei</i> TCTP.

<p>A) Relative expression of TCTP in different tissues of <i>Litopeaeus vannamei</i> determined by Real-time RT-PCR. Total RNA was extracted from haemocyte, heart, intestine, muscle, hepatopancreas, and gill of healthy shrimp respectively. B) Protein level of TCTP in different organs. Protein level was analyzed by Western blot. Loading of the lanes was normalized to levels of β-actin and the experiment is representative of five independent experiments.The calculated densitometry intensities of the respective bands were present as fold of β-actin. The results are expressed as means ± S.D (n = 5).</p

Temporal expression level of TCTP in gills after WSSV infection.

<p>A) Transcripts determined by real-time RT-PCR at different time post WSSV infection. B) TCTP protein level determined by Western blot. Loading of the lanes was normalized to levels of β-actin and the experiment is representative of three independent expriments. The calculated densitometry intensities of the respective bands were present as fold of β-actin. The results are expressed as means ± S.D (n = 3). The asterisk indicates that the expression levels are significantly different (P<0.05). C) Semi-quantitative RT-PCR expression profiles for transcripts encoding β-actin and VP28 of WSSV during time-course infection of WSSV.</p