ROC analysis showing agreement between global motion test and pattern glare test.

<p>ROC curves after the first session (A), the 5^th session (B), and after a break for a month (C). The AUC was plotted as a function of numbers of sessions (D). Orange, sessions in the first visit; purple, after a 1-month break.</p

Stimulus and experimental procedure.

<p>(A) Kinematogram with different levels of coherence with dots moving in the same direction presented as filled one. (B) Experimental procedure. (C) An example of results obtained from a staircase with a 3-down-1-up paradigm.</p

The donkey - a livestock or a pet? (Breeding and use of donkeys in Polabí lowland - a case study)

Donkeys have been useful helpers to people especially at transporting burdens and in agriculture for thousands of years. Nowadays they make livelihoods of rural as well as urban inhabitants over all continents easier. The use of donkeys in agriculture and transport is vanishing in developing countries due to the mechanization. We know only little about how and why people keep breeding of donkeys in developing countries. Theoretical part of the thesis tries to summarize previous basic knowledge about breeding and using donkeys. It focuses on domestication of donkeys, their use in history in different parts of the world, current position of donkeys and partly on relations between men and animals from the view of Human-Animal Studies. A research in a part of Polabí lowland is added. The main practical aim of the thesis is to take a look at the phenomenon of breeding donkeys in the Czech Republic in the present and bring any findings about why do people breed donkeys in modern times in this developing country. Research finds how are donkeys bred, what reasons lead breeders to get donkeys and what their real use is. The thesis contributes to popularization of this theme among experts and laymen and suggests methodology for research of breeding donkeys in other areas as well

Scores on the pattern glare test.

<p>Scores on the pattern glare test.</p

The thresholds to detect global motion after each session.

<p>The thresholds to detect global motion after each session.</p

The thresholds to detect global motion reduced after practice.

<p>Top panels: Line plot illustrating the changes in threshold to detect global motion with training sessions in normal subjects (A) and those with PRVS (B). Gray lines represent individual subjects’ data and colored symbols represent mean values after each session. Bottom panel: probability density plot for the threshold to detect global motion after the 1^st session (C) and the 5^th session (D). Blue: normal subjects; red: subjects with PRVS.</p

Development of a Reference Standard of <i>Escherichia coli</i> DNA for Residual DNA Determination in China

<div><p>This collaborative study developed the first national <i>Escherichia coli</i> (<i>E. coli</i>) DNA reference standard for standardizing quantitative residual DNA assay methods, fluorescence dye (PicoGreen) and quantitative PCR (q-PCR), which were widely employed to measure residual DNA contents of prokaryotic-derived recombinant products. High purity of <i>E. coli</i> strain <i>BL21</i> was extracted by the cetyl triethyl ammonium bromide (CTAB)/phenol chloroform method, analyzed by UV-visible spectrophotometry and electrophoresis, diluted with tris-EDTA (TE) buffer and manually dispensed. Then, with a cooperative calibration among six laboratories, including five manufacturers and one national control laboratory, the concentration of <i>E. coli</i> DNA standard solution was determined as 96.2 μg/mL (95% C.I: 95.5–96.9 μg/mL, CV 3.4%). The candidate showed excellent stability both from accelerated degradation study and real time stability study. The applicability study showed that the <i>E. coli</i> DNA reference could reach the sensitivity of 0.781 ng/mL and 1 fg/μL, respectively, in fluorescent dye and q-PCR assay, and also had good linearity and precision. The consistency of the reference could meet the requirements of the national reference standard. As a conclusion, the candidate material was suitable to serve as a China national standard for <i>E. coli</i> residual DNA determination. The successful establishment of the <i>E. coli</i> DNA standard will facilitate the standardization of quantitative methods for testing residual host cell DNA.</p></div

ROC curves for detecting the risk factors of amblyopia (hyperopia, myopia, astigmatism, anisometropia, and strabismus).

<p>ROC curves for detecting the risk factors of amblyopia (hyperopia, myopia, astigmatism, anisometropia, and strabismus).</p

Sensitivity and specificity to detect AAPOS 2013 defined amblyopia risk factors with Spot cutoff values derived from ROC curves.

<p>Sensitivity and specificity to detect AAPOS 2013 defined amblyopia risk factors with Spot cutoff values derived from ROC curves.</p

Sensitivity and specificity of identifying amblyopia risk factors with AAPOS criteria.

<p>Sensitivity and specificity of identifying amblyopia risk factors with AAPOS criteria.</p