60 research outputs found
Propaganda and Communication of the Islamic State
The thesis concentrates on propaganda from the theoretical point of view that is later applied to the example of the Islamic state. The aim of the thesis is to analyze propaganda of the Islamic State between the years 2006-2016. This period is divided into three spells. The first chapter defines propaganda, its´ tools, targets and pillars. The second chapter focuses on the Islamic state and its´ ideology and the media it uses to spread its´ propaganda. Foreign fighters are a frequent target of the propaganda, therefore, the third chapter focuses on them and the process of their radicalisation. The final chapter applies the theory of propaganda to the Islamic State and identifies the tools and pillars of propaganda in all three time spells
Additional file 7: of Genome-wide association mapping revealed a diverse genetic basis of seed dormancy across subpopulations in rice (Oryza sativa L.)
The Genome-wide association mapping results for Germination Percentage (GP) of the after-ripened seeds (ARS) in whole, Aus, indica and japonica populations. The figure shows neighbor-joining tree, histogram of the phenotypes (GP), quantile-quantile plot of the expected null distribution and the observed P-value and the Manhattan plots of GP of after-ripened seeds in populations using LMM and LR methods. (PDF 823 kb
Imaging Lysosomal pH Alteration in Stressed Cells with a Sensitive Ratiometric Fluorescence Sensor
The organelle-specific pH is crucial
for cell homeostasis. Aberrant
pH of lysosomes has been manifested in myriad diseases. To probe lysosome
responses to cell stress, we herein report the detection of lysosomal
pH changes with a dual colored probe (CM-ROX), featuring a coumarin
domain with “always-on” blue fluorescence and a rhodamine–lactam
domain activatable to lysosomal acidity to give red fluorescence.
With sensitive ratiometric signals upon subtle pH changes, CM-ROX
enables discernment of lysosomal pH changes in cells undergoing autophagy,
cell death, and viral infection
Defining Cancer Cell Bioenergetic Profiles Using a Dual Organelle-Oriented Chemosensor Responsive to pH Values and Electropotential Changes
Cell
fate is largely shaped by combined activity of different types
of organelles, which often feature functionally critical parameters
that succumb to pathological inducers. We herein report the analysis
of cell bioenergetic profiles with a dual organelle-oriented chemosensor
(RC-AMI), partitioning in mitochondria to give blue fluorescence and
in lysosomes to give red fluorescence. Responsive to lysosomal pH
and mitochondrial transmembrane potential (ΔΨ<sub>m</sub>), two parameters crucial to cell bioenergetics, RC-AMI enables dual
colored reporting of lysosomal acidity and ΔΨ<sub>m</sub>, revealing upregulated ΔΨ<sub>m</sub> and imbalance
dramatically shifted favoring ΔΨ<sub>m</sub> over lysosomal
acidity in cancer cells whereas the tendency is reversed in starved
cells. Complementing classical homo-organelle-specific sensors, this
dual organelle-oriented and fluorescently responsive probe offers
a new tool to detect imbalance between lysosomal acidity and mitochondrial
ΔΨ<sub>m</sub>, an index critical for cancer bioenergetics
Table_3_Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures.xlsx
Toon buds, a popular woody vegetable, contain large amounts of nutrients. However, toon buds have strong respiratory metabolism after harvest and are highly prone to decay, resulting in quality deterioration. Low temperature can effectively inhibit postharvest senescence of toon buds. GC-TOF-MS combined with quantitative real-time PCR was used to elucidate the toon bud deterioration mechanism after harvest by analyzing the difference in the relative contents of primary metabolites and their derivatives, and the expression of key genes associated with metabolic pathways in toon buds between low temperature and room temperature storages for 72Â h. Results showed that the ethylene synthesis in toon buds accelerated under room temperature storage, along with significant changes in the primary metabolic pathway. The catabolism of amino acids, fatty acids, and cell membrane phospholipids was accelerated, and the gluconeogenesis synthesis was strengthened. Moreover, the sucrose synthesis was increased, the glycolysis and TCA cycle were broken down, and the pentose phosphate pathway was vigorous. As metabolic intermediates, organic acids were considerably accumulated. Moreover, varieties of toxic compounds were produced in parallel with the activation of aromatic compounds. This work provided a comprehensive understanding of the metabolic regulation, thereby revealing how low and room temperatures differentially influenced the quality deterioration of postharvest toon buds.</p
Table_1_Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures.xlsx
Toon buds, a popular woody vegetable, contain large amounts of nutrients. However, toon buds have strong respiratory metabolism after harvest and are highly prone to decay, resulting in quality deterioration. Low temperature can effectively inhibit postharvest senescence of toon buds. GC-TOF-MS combined with quantitative real-time PCR was used to elucidate the toon bud deterioration mechanism after harvest by analyzing the difference in the relative contents of primary metabolites and their derivatives, and the expression of key genes associated with metabolic pathways in toon buds between low temperature and room temperature storages for 72Â h. Results showed that the ethylene synthesis in toon buds accelerated under room temperature storage, along with significant changes in the primary metabolic pathway. The catabolism of amino acids, fatty acids, and cell membrane phospholipids was accelerated, and the gluconeogenesis synthesis was strengthened. Moreover, the sucrose synthesis was increased, the glycolysis and TCA cycle were broken down, and the pentose phosphate pathway was vigorous. As metabolic intermediates, organic acids were considerably accumulated. Moreover, varieties of toxic compounds were produced in parallel with the activation of aromatic compounds. This work provided a comprehensive understanding of the metabolic regulation, thereby revealing how low and room temperatures differentially influenced the quality deterioration of postharvest toon buds.</p
Table_2_Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures.xlsx
Toon buds, a popular woody vegetable, contain large amounts of nutrients. However, toon buds have strong respiratory metabolism after harvest and are highly prone to decay, resulting in quality deterioration. Low temperature can effectively inhibit postharvest senescence of toon buds. GC-TOF-MS combined with quantitative real-time PCR was used to elucidate the toon bud deterioration mechanism after harvest by analyzing the difference in the relative contents of primary metabolites and their derivatives, and the expression of key genes associated with metabolic pathways in toon buds between low temperature and room temperature storages for 72Â h. Results showed that the ethylene synthesis in toon buds accelerated under room temperature storage, along with significant changes in the primary metabolic pathway. The catabolism of amino acids, fatty acids, and cell membrane phospholipids was accelerated, and the gluconeogenesis synthesis was strengthened. Moreover, the sucrose synthesis was increased, the glycolysis and TCA cycle were broken down, and the pentose phosphate pathway was vigorous. As metabolic intermediates, organic acids were considerably accumulated. Moreover, varieties of toxic compounds were produced in parallel with the activation of aromatic compounds. This work provided a comprehensive understanding of the metabolic regulation, thereby revealing how low and room temperatures differentially influenced the quality deterioration of postharvest toon buds.</p
Image_1_Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures.tif
Toon buds, a popular woody vegetable, contain large amounts of nutrients. However, toon buds have strong respiratory metabolism after harvest and are highly prone to decay, resulting in quality deterioration. Low temperature can effectively inhibit postharvest senescence of toon buds. GC-TOF-MS combined with quantitative real-time PCR was used to elucidate the toon bud deterioration mechanism after harvest by analyzing the difference in the relative contents of primary metabolites and their derivatives, and the expression of key genes associated with metabolic pathways in toon buds between low temperature and room temperature storages for 72Â h. Results showed that the ethylene synthesis in toon buds accelerated under room temperature storage, along with significant changes in the primary metabolic pathway. The catabolism of amino acids, fatty acids, and cell membrane phospholipids was accelerated, and the gluconeogenesis synthesis was strengthened. Moreover, the sucrose synthesis was increased, the glycolysis and TCA cycle were broken down, and the pentose phosphate pathway was vigorous. As metabolic intermediates, organic acids were considerably accumulated. Moreover, varieties of toxic compounds were produced in parallel with the activation of aromatic compounds. This work provided a comprehensive understanding of the metabolic regulation, thereby revealing how low and room temperatures differentially influenced the quality deterioration of postharvest toon buds.</p
La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]
02 mai 18731873/05/02 (N2551)
Additional file 18: Table S16. of Exploring the rice dispensable genome using a metagenome-like assembly strategy
Functional enrichment analysis of 6302 reference genes involved in the formation of non-reference sequences through exon/intron shuffling. The hmm accession for all the 6302 reference genes was extracted, and the number of genes with a specific hmm accession involved in the formation of non-reference sequences through exon/intron shuffling were compared with the whole genome level to find enriched accessions. In total, 3581 of these 6302 genes and 33,581 genes of the whole genome were annotated by Pfam. (DOC 40Ă‚Â kb
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