The effect of robot-assisted gait training for patients with spinal cord injury: a systematic review and meta-analysis

BackgroundWith the aging of the global population, Spinal injuries are often prone to occur and affect human health. The development of technology has put robots on the stage to assist in the treatment of spinal injuries.MethodsA comprehensive literature search were carried out in multiple databases, including PubMed, Medline (Ovid), Web of Science, Cochrane, Embase, Scopus, CKNI, Wang fang, VIP database, Sino Med, Clinical Trails until 20th, June, 2023 to collect effect of robot-assisted gait training for patients with spinal cord injury patients. Primary outcome includes any changes of gait distance and gait speed. Secondary outcomes include any changes in functions (Such as TUG, Leg strength, 10 MWT) and any advent events. Data were extracted from two independent individuals and Cochrane Risk of Bias tool version 2.0 was assessed for the included studies. Systematic review and meta-analysis were performed by RevMan 5.3 software.Results11 studies were included in meta-analysis. The result showed that gait distance [WMD = 16.05, 95% CI (−15.73, 47.83), I2 = 69%], gait speed (RAGT vs. regular treatment) [WMD = 0.01, 95% CI (−0.04, 0.05), I2 = 43%], gait speed (RAGT vs. no intervention) [WMD = 0.07, 95% CI (0.01, 0.12), I2 = 0%], leg strength [WMD = 0.59, 95% CI (−1.22, 2.40), I2 = 29%], TUG [WMD = 9.25, 95% CI (2.76, 15.73), I2 = 74%], 10 MWT [WMD = 0.01, 95% CI (−0.15, 0.16), I2 = 0%], and 6 MWT [WMD = 1.79, 95% CI (−21.32, 24.90), I2 = 0%].ConclusionRobot-assisted gait training seems to be helpful for patients with spinal cord to improve TUG. It may not affect gait distance, gait speed, leg strength, 10 MWT, and 6 MWT

Improving Visual Comfort and Health through the Design of a Local Shading Device

As people develop higher standards for the beauty of urban architecture, more and more architectural designs are exploring sources of natural lighting, such as glass curtain walls and glass domes. However, the pursuit of architectural design aesthetics introduces glare into buildings, which can be uncomfortable and even dangerous for health. Scholars in related fields have carried out many theoretical studies and design activities based on this problem. In this study, we focus on issues of glare in public buildings, aiming to improve light comfort by solving this problem. First, we propose an improved design strategy based on IDEO design thinking by adding the step of diverging from the design scheme. Second, guided by the results of a questionnaire survey and light environment simulations, we avoided the subjective simplicity of the traditional design process. Third, taking the main library of Shanghai Jiao Tong University as an example, we designed a movable sunshade that could effectively reduce glare effects and improve visual comfort, which improved the light comfort in public buildings. The simulation results show that the proposed design can be useful in buildings to effectively control glare and improve visual comfort and health

Manual Acupuncture Suppresses the Expression of Proinflammatory Proteins Associated with the NLRP3 Inflammasome in the Hippocampus of SAMP8 Mice

Objective. To investigate the effect of manual acupuncture (MA) on NLRP3 inflammasome-related proteins. Methods. SAMP8 mice were randomly divided into Alzheimer’s disease (AD) group, the MA group, and the medicine (M) group. Mice in the M group were treated with donepezil hydrochloride at 0.65 μg/g. In the MA group, MA was applied on Baihui (GV20) and Yintang (GV29) for 20 min and then pricked at Shuigou (GV26). The Morris water maze was applied to assess spatial learning and memory. Immunohistochemical staining and western blot analysis were used to observe the expression of NLRP3 inflammasome-related proteins. Results. Compared with the normal (N) control group, spatial learning and the memory capabilities of the AD group significantly decreased (p<0.01). The number of NLRP3, ASC, Caspase-1, and IL-1β positively stained cells in the AD group was higher than the N group, and the relative expression levels of the above proteins were significantly higher than those in the N group (p<0.01). These changes were reversed by both MA and donepezil (p<0.01). Conclusion. MA can improve the learning and memory capabilities of SAMP8 mice. The negative regulation of the NLRP3/Caspase-1 pathway in the hippocampus may be a possible mechanism of MA in the treatment of AD

Experimental investigation on phase inversion point and flow characteristics of heavy crude oil-water flow

The phase inversion point is of great significance for drag reduction in the flow of heavy crude oil. In this study, we used 25-mm inner diameter (ID) stainless steel pipes to investigate the oil-water flow characteristics of four heavy oils at five temperatures (90, 85, 80, 75 and 70 &deg;C). The effects of temperature, velocity, and oil viscosity on the phase inversion point of heavy oil-water flow were studied. The viscosity of the experimental oil ranges from 358 to 4995 mPa&middot;s. The phase inversion model of Yao and Gong (2006) was modified to fit high-viscosity oils. The proposed model was verified with pipe flow experimental data from various heavy oilfields. This study found that the effect of temperature on the phase inversion point of heavy crude oil is almost negligible. The flow pattern with the largest pressure drop during heavy oil-water flow is Int, and the flow patterns with a smaller pressure drop include semi-Anw, Anw, and DAnw. The water content at the inversion point of heavy oil-water flow is mostly between 45% and 70%. As the viscosity increases, the water fraction at the inversion point decreases. A high velocity helps the water maintain a continuous phase. The occurrence of phase inversion requires the mixture velocity to be maintained above a specific value, which should be high enough for the flow pattern to transition to semi-annular flow

Mast cells are important regulator of acupoint sensitization via the secretion of tryptase, 5-hydroxytryptamine, and histamine

<div><p>Mast cells (MCs) play a crucial role in mediating the establishment of networks among the circulatory, nervous and immune system at acupoints. However, the changes which occur in MCs during acupoint sensitization, i.e. the dynamic transformation of an acupoint from a "silenced" to an "activated" status, remain uncharacterized. To investigate the morphological and functional changes of MCs as an aid to understanding the cellular mechanism underlying acupoint sensitization, a rat model of knee osteoarthritis (OA) was induced by an injection of mono-iodoacetate (MIA) on day 0. On day 14, toluidine blue and immunofluorescence staining were used to observe the recruitment and degranulation of MCs and the release of mast cell co-expressed mediators: tryptase, 5-hydroxytryptamine (5-HT) and histamine (HA) at the acupoints Yanglingquan (GB34), Heding (EX-LE2) and Weizhong (BL40). Results showed that the number of MCs as well as the percentages of degranulated and extensively degranulated MCs at the acupoints GB34 and EX-LE2 in the light (A), mild (B), heavy (C) osteoarthritis groups were larger than those in the normal control (N) and normal saline (NS) groups (p < 0.01). Comparisons among the A, B and C groups suggested that the number and the degranulation extent of the MCs at the acupoints GB34 and EX-LE2 were positively correlated with the severity of the disease. Some MCs in the A, B and C group showed the release of 5-HT, HA, and tryptase in degranulation at the acupoints GB34 and EX-LE2. Such changes in MCs were not observed at the acupoint BL40. In conclusion, this study confirmed that acupoint sensitization is associated with the increase in recruitment and degranulation levels of MCs on a acupoint-specific and disease severity-dependent manner. The release of tryptase, 5-HT, and HA during MC degranulation is likely to be one of the cellular mechanisms occurring during acupoint sensitization.</p></div

MOESM3 of Characterization and potential role of microRNA in the Chinese dominant malaria mosquito Anopheles sinensis (Diptera: Culicidae) throughout four different life stages

Additional file 3: Table S3. Target gene candidates for known and novel miRNAs in An. sinensis

MOESM4 of Characterization and potential role of microRNA in the Chinese dominant malaria mosquito Anopheles sinensis (Diptera: Culicidae) throughout four different life stages

Additional file 4: Table S4. Developmental target gene candidates for 32 key miRNAs during development in An. sinensis

Representative immunofluorescence staining of mast cell tryptase (green), and co-expressed HA (red) in the N and C group.

<p>The MCs-produced tryptase, mainly expressed in the cytoplasm, was green fluorescence labeled and the co-expression of HA was red fluorescence labeled. The positively stained cells, degranulated MCs are shown by white, red arrows. Scale bar is 40 μm.</p

Assessment of suitability of the MIA-induced knee osteoarthritis rat model.

<p>(A-E) Representative images from the safranine-O and fast green staining of the N group, the NS group, the A group, the B group and the C group. Scale bar is 100 μm. (F) Comparison of the OA scores as assessed on articular cartilage of model rats in each group. Data were showed as means ± SD, n = 6, ◇◇P < 0.01 vs the N group, ◆◆P < 0.01 vs the NS group, ΔΔP < 0.01 vs the A group, ^▲▲P < 0.01 vs the B group.</p