Increased expression of two alternative spliced variants of CD1d molecule in human gastric cancer

Background: CD1d presents glycolipid antigens to invariant natural killer T (iNKT) cells. The role of CD1d in the development of peptic ulcer and gastric cancer has not been revealed, yet. Objective: To clarify the expression of alternatively spliced variants of CD1d in peptic ulcer and gastric cancer. Methods: Patients with dyspepsia were selected and divided into three groups of non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), and gastric cancer (GC), according to their endoscopic and histopathological examinations. H. pylori infection was diagnosed by rapid urease test and histopathology. The expression levels of V2, V4, and V5 spliced variants of CD1d molecule were determined by quantitative Reverse Transcriptase PCR. Results: Relative gene expression levels of V4 were higher in GC patients (n=37) than those in NUD (n=49) and PUD (n=51) groups (p<0.05 and p<0.01, respectively). Moreover, GC patients showed higher expression levels of V5 compared to NUD and PUD groups (p<0.001 and p<0.001, respectively). Positive correlation coefficients were attained between V4 and V5 expression in patients with PUD (r=0.734, p<0.0001) and GC (r=0.423, p<0.01), but not in patients with NUD. Among NUD patients, the expression levels of V4, but not V5, were higher in H. pylori-positive patients than in H. pylori-negative ones (p<0.01). Conclusion: Collectively, both membrane-bound (V4) and soluble (V5) isoforms of CD1d were over-expressed in gastric tumor tissues, suggesting that they are involved in anti-tumor immune responses. © 2015, Shiraz University of Medical Sciences. All rights reserved

Nash-Bargaining-Based Models for Matching Markets: One-Sided and Two-Sided; Fisher and Arrow-Debreu

This paper addresses two deficiencies of models in the area of matching-based market design. The first arises from the recent realization that the most prominent solution that uses cardinal utilities, namely the Hylland-Zeckhauser (HZ) mechanism [Hylland and Zeckhauser, 1979], is intractable; computation of even an approximate equilibrium is PPAD-complete [Vazirani and Yannakakis, 2021; Chen et al., 2021]. The second is the extreme paucity of models that use cardinal utilities, in sharp contrast with general equilibrium theory. Our paper addresses both these issues by proposing Nash-bargaining-based matching market models. Since the Nash bargaining solution is captured by a convex program, efficiency follow; in addition, it possesses a number of desirable game-theoretic properties. Our approach yields a rich collection of models: for one-sided as well as two-sided markets, for Fisher as well as Arrow-Debreu settings, and for a wide range of utility functions, all the way from linear to Leontief. We also give very fast implementations for these models which solve large instances, with n = 2000, in one hour on a PC, even for a two-sided matching market. A number of new ideas were needed, beyond the standard methods, to obtain these implementations

Expression levels of vascular endothelial growth factors A and C in patients with peptic ulcers and gastric cancer

Purpose: Vascular endothelial growth factor (VEGF) is one of the most important growth factors for metastatic tumors. To clarify the role of VEGF-A and C in patients with peptic ulcer disease (PUD) or gastric cancer (GC), we evaluated the expression levels of these two molecules. We also analyzed the effect of Helicobacter pylori infection on VEGF-A and C expression levels

Optimization and One-Step Purification of Recombinant V Antigen Production from Yersinia pestis

The purpose of this study was to develop an efficient and inexpensive method for the useful production of recombinant protein V antigen, an important virulence factor for Yersinia pestis. To this end, the synthetic gene encoding the V antigen was subcloned into the downstream of the intein (INT) and chitin-binding domain (CBD) from the pTXB1 vector using specific primers. In the following, the produced new plasmid, pTX-V, was transformed into E. coli ER2566 strain, and the expression accuracy was confirmed using electrophoresis and Western blotting. In addition, the effects of medium, inducer, and temperature on the enhancement of protein production were studied using the Taguchi method. Finally, the V antigen was purified by a chitin affinity column using INT and CBD tag. The expression was induced by 0.05 mM IPTG at 25 °C under optimal conditions including TB medium. It was observed that the expression of the V-INT�CBD fusion protein was successfully increased to more than 40 of the total protein. The purity of V antigen was as high as 90. This result indicates that V antigen can be produced at low cost and subjected to one-step purification using a self-cleaving INT tag. © 2020, Springer Science+Business Media, LLC, part of Springer Nature

Delay sensitive and power-aware SMDP-based connection admission control mechanism in cognitive radio sensor networks

© 2017 Elsevier B.V. Due to the opportunistically resource usage of users in cognitive radio sensor networks (CRSNs), the availability of network resources is highly variable. Therefore, admission control is an essential mechanism to manage the traffic of cognitive radio users in order to satisfy the quality of service (QoS) requirements of applications. In this study, a connection admission control (CAC) mechanism is introduced to satisfy the requirements of delay sensitivity and power consumption awareness. This proposed mechanism is modeled through a semi Markov decision process (SMDP) and a linear programming problem is derived with the aim of obtaining the optimal policy to control the traffic of CRSNs and achieving maximum reward. The number of required channels at each network state is estimated through a graph coloring approach. An end to end delay constraint is defined for the optimization problem which is inspired from Kleinrock independence approximation. Furthermore, a power-aware weighting method is proposed for this mechanism. We conduct different simulation-based scenarios to investigate the performance of the proposed mechanism. The experimental results demonstrate the efficiency of this SMDP-based mechanism in comparison to the last CAC mechanism in CRSNs

An HTTP/2-based adaptive streaming framework for 360° virtual reality videos

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