TERT promoter mutations and telomere length in adult malignant gliomas and recurrences.

In this report on 303 gliomas we show the highest frequency of TERT promoter mutations in gliobastomas (80%) followed by oligodendrogliomas (70%) and astrocytomas (39%). We observed positive association between TERT promoter and IDH mutations in oligodendroglial tumors (OR = 26.3; 95% CI 2.5-250.2) and inverse association in primary glioblastomas (OR = 0.13; 95% CI 0.03-0.58). Tumors with TERT promoter mutations compared to those without showed increased TERT transcription; we also showed difference in the transcription levels due to the two main mutations. Tumors with TERT promoter mutations had shorter telomeres than those without. The patients with only TERT promoter mutations showed worst survival (median survival 14.6 months) and patients with both IDH and TERT promoter mutations showed best survival (246.5 months). In patients with astrocytoma, the TERT promoter mutations only associated with poor survival (P < 0.0001); IDH mutations and 1p/19q deletions associated with increased survival (P = 0.0004). TERT promoter mutations in low grade gliomas associated with reduced progression free survival (HR 10.2; 95% CI 1.9 - 55.9). While our data affirm the role of TERT promoter mutations in glial tumors, effects on transcription and telomere length emphasise the importance of telomere biology in disease genesis and outcome

High-order approximate solutions of strongly nonlinear cubic-quintic Duffing oscillator based on the harmonic balance method

In this paper, a new reliable analytical technique has been introduced based on the Harmonic Balance Method (HBM) to determine higher-order approximate solutions of the strongly nonlinear cubicquintic Duffing oscillator. The application of the HBM leads to very complicated sets of nonlinear algebraic equations. In this technique, the high-order nonlinear algebraic equations are approximated in the form of a power series solution, and this solution produces desired results even for small as well as large amplitudes of oscillation. Moreover, a suitable truncation formula is found in which the solution measures better results than existing results and it saves a lot of calculation. It is highly noteworthy that using the proposed technique, the third-order approximate solutions gives an excellent agreement as compared with the numerical solutions (considered to be exact). The proposed technique is applied to the strongly nonlinear cubic-quintic Duffing oscillator to reveals its novelty, reliability and wider applicability

Facebook use and its predictive factors among students: Evidence from a lower- and middle-income country, Bangladesh

Background:Facebook is a popular social networking site in the modern world. It has an adverse effect such as impairing daily health and psychological health and also interpersonal relationships when the use becomes problematic.AimsTo examine problematic Facebook use (PFU) and its predictors among Bangladeshi students during the COVID-19 pandemic.MethodA cross-sectional online survey was conducted among 601 Bangladeshi students and collected data related to socio-demographic information, behavioral health, internet use behavior, depression, anxiety and problematic Facebook use [assessed using the Bergen Facebook Addiction Scale (BFAS)]. The data were analyzed using descriptive (frequencies and percentages) and inferential statistics (independent sample t-tests, one-way ANOVAs, correlations, and multivariable linear regression).ResultsThe results indicated that 29.1% of participants were problematic Facebook users (using cutoff ≥18 out of 30). Medical college students had higher mean score on PFU than other students (p &lt; 0.001). In addition, the mean score of PFU was significantly higher among the students who were in a relationship (p = 0.001), did not engage in physical activity (p &lt; 0.001), used the internet more than 5 h per day (p &lt; 0.001), used social media (p &lt; 0.001), and had depression or anxiety symptoms (p &lt; 0.001). PFU was significantly associated with depression and anxiety among the whole sample. Predictive factors for PFU included relationship status, daily internet use time, gaming, social media use, depression, and anxiety. The model predicted almost 33.2% variance for PFU.ConclusionsFindings suggest interventions should be implemented for students with a special focus on medical students who had higher score of PFU than other types of students

An analytical technique to obtain higher-order approximate periods for nonlinear oscillator

ABSTRACT: This paper presents simulation results of the influence of wide range modulation index values ( ) in carrier-based PWM strategy for application in generating the stepped waveform. The waveform is tested for application in single-phase half-bridge modular multilevel converters (MMCs) topology. The results presented in this paper include a variation of the fundamental component (50 Hz) in the voltage output.  It also studies total harmonic distortion of the output voltage (THDv) and the output current (THDi) when the modulation index is changed over the linear-modulation region, 0 < < 1. It also explores the effect of a modulation index greater than 1. Moreover, different output voltage shapes, as a consequence of varied on MMCs, are also illustrated for showing the effect of varying the value of on sub-module of MMCs. ABSTRAK: Penulisan ini berkenan simulasi pengaruh pelbagai nilai indeks modulasi     ( ) dalam strategi PWM berasaskan aplikasi dalam menghasilkan bentuk gelombang yang bertingkat. Bentuk gelombang ini diuji untuk aplikasi dalam topologi MMCs. Penilaian dan hasil dari artikle ini termasuk variasi komponen asas (50 Hz) dalam voltan keluar. Ia juga meneliti jumlah penyelarasan harmonik voltan keluar (THDv) dan arus keluaran (THDi) apabila indeks modulasi ditukar dalam rantau modulasi linear, 0 < <1. Ia juga meneroka kesan indeks modulasi lebih daripada 1. Selain itu, bentuk voltan keluar yang berbeza sebagai akibat dari pelbagai  pada MMCs juga digambarkan untuk menunjukkan kesan berbeza-beza nilai  pada sub-modul MMCs

An analytical approximation technique for the duffing oscillator based on the energy balance method

In this paper, an analytical approximation technique has been presented of obtaining higher-order approximate solutions for highly nonlinear Duffing oscillator based on the energy balance method (EBM). Higher-order approximate natural frequencies have been obtained in a novel analytical way. The accuracy of the solution method is evaluated within as error analysis. It is highly remarkable that using the presented technique, the approximation solutions produces desired results even for large oscillation as compared with the exact ones. Moreover, the solution method yields much better results than existing solutions after using a suitable truncation formula. The presented technique is applied to well-known Duffing oscillator to illustrate its novelty, reliability and wider applicabilit

A population-based nationwide data set concerning the COVID-19 pandemic and serious psychological consequences in Bangladesh

This paper presents the dataset concerning knowledge, pre- ventive behavior, psychological consequences, and suicidal behavior regarding the COVID-19 pandemic in Bangladesh. Data were collected through an online based cross-sectional survey between April 1 and April 10 in 64 districts at the early stage of the COVID-19 pandemic in Bangladesh. A total of 10,067 participants’ data were recruited for analysis. The survey contained items concerning (i) socio-demographic in- formation, (ii) knowledge concerning COVID-19, (iii) behav- ior towards COVID-19, (iv) lockdown and economic issues, (v) assessment of fear of COVID-19, (vi) assessment of in- somnia, (vii) assessment of depression, and (viii) assessment of suicidal ideation. Data were analyzed utilizing SPSS (ver- sion 22) and are represented as frequencies and percentages based on responses to the whole survey. Given that the data were collected across the whole nation, government authori- ties and healthcare policymakers can use the data to develop various models and/or policies regarding preventive strate- gies and help raise awareness through health education to- wards COVID-19

Development of anthracnose disease resistance and heat tolerance chili through conventional breeding and molecular approaches: a review

Chili (Capsicum annuum L.) is the popular spicy vegetable crops belonging to family Solanaceae. Chili peppers are known for their pungency characteristic due to the presence of capsaicinoids that classifies them into hot or sweet pepper. Chili is used as spices, folk remedies for diseases, vegetables, and coloring agent showing a diverse role in human’s life. However, its production is hampered by different biotic stress and abiotic factors. Similarly, the unavailability of high yielding varieties, high temperature, and disease incidence, particularly, anthracnose disease, are the major constraints responsible for the low production of chili pepper. The advents of molecular markers, advancement in quantitative trait loci by classical genetic analysis, and conventional breeding have shown the number of genes for many important and major traits. While the newly developed genotyping technologies and next generation sequencing have led to the discovery of molecular basis for economic important characters in the chili genome and generate large scale data for genomic resources. Based on this background, this review summarizes progress in the development of anthracnose disease-resistant and heat-tolerant chili genotypes through conventional breeding and molecular approaches. This review would help plant breeders in understanding the phenotypic and genetic make-up of capsicum genotypes and provides opportunities for pyramiding two respected genes with the help of diversified phenotypic and molecular marker evaluation

Sleep Problems in Children with Autism Spectrum Disorder in Bangladesh: A Case–Control Study

Background: Sleep problems in children with Autism Spectrum Disorder (ASD) are highly prevalent, but little information is available on this issue in low- to middle-income countries (LMIC) such as Bangladesh. Therefore, the present study investigated the prevalence and socio-demographic determinants of ASD sleep disturbances in a comparison with typically developing children (TDC). Methods: A cross-sectional interview study was carried out within a total of 446 Bangladeshi mothers, whose children’s mean age was 8.1±2.9 years (151 ASD [8.5±2.7 years] and 295 TDC [7.9±2.9 years]); in addition to socio-demographics, the Child Sleep Habit Questionnaire (CSHQ) was used, and a cut-off score of 41 out of 93 points considered as reflecting sleep problems. Results: About 89.7% of the children reported having problems in sleep, with ASD reporting higher frequency vs TDC (94.00% vs 87.50%; χ2=4.678, p=0.031). The overall mean CSHQ score was 48.7±7.6 in total sample, whereas ASD children reported higher scores compared to TDCs (50.9±8.1 vs 47.5±7.0, p<0.001). Similarly, subscales of CSHQ such as sleep duration (4.23±1.56 vs 3.90±1.31, p=0.017), sleep anxiety (7.23±2.05 vs 6.45 ±1.92, p<0.001), night waking (3.82±1.07 vs 3.17±1.89, p<0.001), parasomnias (8.86±2.06 vs 7.85±2.27, p<0.001), and sleep disordered breathing (4.02±2.92 vs 3.43±2.07, p=0.014) were more problematic among ASD compared to TDC. Lastly, 28.5% of ASD reported taking sleep-related medications vs 0.3% for TDC (n=1). Conclusion: Bangladeshi ASD children are highly likely to manifest sleep disturbances, which warrant urgent implementation of parental educational and support programs to mitigate the impact of sleep problems in ASD families

Analyses of Genetic Variations of Glutathione S-Transferase Mu1 and Theta1 Genes in Bangladeshi Tannery Workers and Healthy Controls

Glutathione S-transferases (GSTs) belong to a group of multigene detoxification enzymes, which defend cells against oxidative stress. Tannery workers are at risk of oxidative damage that is usually detoxified by GSTs. This study investigated the genotypic frequencies of GST Mu1 (GSTM1) and GST Theta1 (GSTT1) in Bangladeshi tannery workers and healthy controls followed by their status of oxidative stress and total GST activity. Of the 188 individuals, 50.0% had both GSTM1 and GSTT1 (+/+), 12.2% had GSTM1 (+/−), 31.4% had GSTT1 (−/+) alleles, and 6.4% had null genotypes (−/−) with respect to both GSTM1 and GSTT1 alleles. Among 109 healthy controls, 54.1% were double positive, 9.2% had GSTM1 allele, 32.1% had GSTT1 allele, and 4.6% had null genotypes. Out of 79 tannery workers, 44.3% were +/+, 16.8% were +/−, 30.5% were −/+, and 8.4% were −/−. Though the polymorphic genotypes or allelic variants of GSTM1 and GSTT1 were distributed among the study subjects with different frequencies, the differences between the study groups were not statistically significant. GST activity did not vary significantly between the two groups and also among different genotypes while level of lipid peroxidation was significantly higher in tannery workers compared to controls irrespective of their GST genotypes

Construction of copy number variation landscape and characterization of associated genes in a Bangladeshi cohort of neurodevelopmental disorders

Introduction: Copy number variations (CNVs) play a critical role in the pathogenesis of neurodevelopmental disorders (NDD) among children. In this study, we aim to identify clinically relevant CNVs, genes and their phenotypic characteristics in an ethnically underrepresented homogenous population of Bangladesh. Methods: We have conducted chromosomal microarray analysis (CMA) for 212 NDD patients with male to female ratio of 2.2:1.0 to identify rare CNVs. To identify candidate genes within the rare CNVs, gene constraint metrics [i.e., “Critical-Exon Genes (CEGs)”] were applied to the population data. Autism Diagnostic Observation Schedule-Second Edition (ADOS-2) was followed in a subset of 95 NDD patients to assess the severity of autism and all statistical tests were performed using the R package. Results: Of all the samples assayed, 12.26% (26/212) and 57.08% (121/212) patients carried pathogenic and variant of uncertain significance (VOUS) CNVs, respectively. While 2.83% (6/212) patients’ pathogenic CNVs were found to be located in the subtelomeric regions. Further burden test identified females are significant carriers of pathogenic CNVs compared to males (OR = 4.2; p = 0.0007). We have observed an increased number of Loss of heterozygosity (LOH) within cases with 23.85% (26/109) consanguineous parents. Our analyses on imprinting genes show, 36 LOH variants disrupting 69 unique imprinted genes and classified these variants as VOUS. ADOS-2 subset shows severe social communication deficit (p = 0.014) and overall ASD symptoms severity (p = 0.026) among the patients carrying duplication CNV compared to the CNV negative group. Candidate gene analysis identified 153 unique CEGs in pathogenic CNVs and 31 in VOUS. Of the unique genes, 18 genes were found to be in smaller (<1 MB) focal CNVs in our NDD cohort and we identified PSMC3 gene as a strong candidate gene for Autism Spectrum Disorder (ASD). Moreover, we hypothesized that KMT2B gene duplication might be associated with intellectual disability. Conclusion: Our results show the utility of CMA for precise genetic diagnosis and its integration into the diagnosis, therapy and management of NDD patients