Analysis of Expressed Sequence Tags from the Fungus Aspergillus oryzae Cultured Under Different Conditions

We performed random sequencing of cDNAs from nine biologically or industrially important cultures of the industrially valuable fungus Aspergillus oryzae to obtain expressed sequence tags (ESTs). Consequently, 21 446 raw ESTs were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). Among all contigs, 5491 (72.4%) were derived from only a particular culture. These included 4735 (62.4%) singletons, i.e. lone ESTs overlapping with no others. These data showed that consideration of culture grown under various conditions as cDNA sources enabled efficient collection of ESTs. BLAST searches against the public databases showed that 2953 (38.9%) of the EST contigs showed significant similarities to deposited sequences with known functions, 793 (10.5%) were similar to hypothetical proteins, and the remaining 3843 (50.6%) showed no significant similarity to sequences in the databases. Culture-specific contigs were extracted on the basis of the EST frequency normalized by the total number for each culture condition. In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories

Replication Data for: “No Sorting, No Advantage: Regression Discontinuity Estimates of Incumbency Advantage in Japan”

While the existing literature has identified a sizable incumbency advantage in single-member district (SMD) races in developed democracies, we argue that some political and institutional contexts of Japan's Lower House elections would undermine the incumbency advantage. Our regression discontinuity (RD) analysis indeed shows little advantage, and further examination suggests this as largely due to the “best-loser” provision in Japan's mixed-member system, which gives a loser of SMD competition a chance to be a “resurrected” incumbent. We also show no evidence of sorting – i.e., systematic difference between bare winners and bare losers – in close SMD races and thus add further evidence to support the methodological argument that the election RD analysis is a viable and promising research design

The simple and rapid detection of specific

PCR products from bacterial genomes using Zn finger protein

07 「『海外神社』跡地に関するデータベース」構築について

第2部　景観に刻印された人間の諸活動の痕跡を尋ねる―「海外神社」跡地とそのデータベース