802 research outputs found
Stability of chronic intrafascicular electrode recordings
Journal ArticleWe have implanted intrafascicular electrodes in peripheral nerves of cats and have recorded neural activity from a small population of nerve fibers for as long as six months, suggesting that these electrodes can be used on a chronic basis to acquire information about limb position, muscle force and skin contact
Exclusion of Stellar Companions to Exoplanet Host Stars
Given the frequency of stellar multiplicity in the solar neighborhood, it is
important to study the impacts this can have on exoplanet properties and
orbital dynamics. There have been numerous imaging survey projects established
to detect possible low-mass stellar companions to exoplanet host stars. Here we
provide the results from a systematic speckle imaging survey of known exoplanet
host stars. In total, 71 stars were observed at 692~nm and 880~nm bands using
the Differential Speckle Survey Instrument (DSSI) at the Gemini-North
Observatory. Our results show that all but 2 of the stars included in this
sample have no evidence of stellar companions with luminosities down to the
detection and projected separation limits of our instrumentation. The
mass-luminosity relationship is used to estimate the maximum mass a stellar
companion can have without being detected. These results are used to discuss
the potential for further radial velocity follow-up and interpretation of
companion signals.Comment: 11 pages, 4 figures, 3 tables, accepted for publication in A
Masses, Luminosities, and Orbital Coplanarities of the mu Orionis Quadruple Star System from PHASES Differential Astrometry
mu Orionis was identified by spectroscopic studies as a quadruple star
system. Seventeen high precision differential astrometry measurements of mu Ori
have been collected by the Palomar High-precision Astrometric Search for
Exoplanet Systems (PHASES). These show both the motion of the long period
binary orbit and short period perturbations superimposed on that caused by each
of the components in the long period system being themselves binaries. The new
measurements enable the orientations of the long period binary and short period
subsystems to be determined. Recent theoretical work predicts the distribution
of relative inclinations between inner and outer orbits of hierarchical systems
to peak near 40 and 140 degrees. The degree of coplanarity of this complex
system is determined, and the angle between the planes of the A-B and Aa-Ab
orbits is found to be 136.7 +/- 8.3 degrees, near the predicted distribution
peak at 140 degrees; this result is discussed in the context of the handful of
systems with established mutual inclinations. The system distance and masses
for each component are obtained from a combined fit of the PHASES astrometry
and archival radial velocity observations. The component masses have relative
precisions of 5% (component Aa), 15% (Ab), and 1.4% (each of Ba and Bb). The
median size of the minor axes of the uncertainty ellipses for the new
measurements is 20 micro-arcseconds. Updated orbits for delta Equulei, kappa
Pegasi, and V819 Herculis are also presented.Comment: 12 Pages, Accepted for publication in A
Unusual explosive growth of a squamous cell carcinoma of the scalp after electrical burn injury and subsequent coverage by sequential free flap vascular connection – a case report
BACKGROUND: Squamous cell carcinomos may arise from chronic ulcerating wounds in scars, most commonly postburn scars. Tumour growth usually takes place over months to years. Localization on the scalp is a relatively rare condition. CASE PRESENTATION: This report presents the case of a 63-year-old man with chronic ulceration of a postburn scar of the scalp due to an electrical burn 58 years ago. Sudden tumour growth started within weeks and on presentation already had extended through the skull into frontal cortex. After radical tumour resection, defect was covered with a free radial forearm flap. Local recurrence occurred 6 weeks later. Subsequent wide excision including discard of the flap and preservation of the radial vessels was followed by transfer of a free latissimus dorsi muscle flap, using the radial vessels of the first flap as recipient vessels. The patient received radiotherapy post-operatively. There were no problems with flap survivals or wound healing. Due to rapidly growing recurrence the patient died 2 months later. CONCLUSION: Explosive SCC tumour growth might occur in post-burn scars after more than 50 years. As a treatment option the use of sequential free flap connections might serve in repeated extensive tumour resections, especially in the scalp region, where suitable donor vessels are often located in distance to the defect
Observations of Binary Stars with the Differential Speckle Survey Instrument. II. Hipparcos Stars Observed in 2010 January and June
The results of 497 speckle observations of Hipparcos stars and selected other targets are presented. Of these, 367 were resolved into components and 130 were unresolved. The data were obtained using the Differential Speckle Survey Instrument at the WIYN 3.5 m Telescope. (The WIYN Observatory is a joint facility of the University of Wisconsin-Madison, Indiana University, Yale University, and the National Optical Astronomy Observatories.) Since the first paper in this series, the instrument has been upgraded so that it now uses two electron-multiplying CCD cameras. The measurement precision obtained when comparing to ephemeris positions of binaries with very well known orbits is approximately 1-2 mas in separation and better than 0°.6 in position angle. Differential photometry is found to be in very good agreement with Hipparcos measures in cases where the comparison is most relevant. We derive preliminary orbits for two systems
Differential gene expression during compensatory sprouting of dendrites in the auditory system of the cricket Gryllus bimaculatus
Neurones that lose their presynaptic partners because of injury usually retract or die. However, when the auditory interneurones of the cricket Gryllus bimaculatus are denervated, dendrites respond by growing across the midline and forming novel synapses with the opposite auditory afferents. Suppression subtractive hybridization was used to detect transcriptional changes 3 days after denervation. This is a stage at which we demonstrate robust compensatory dendritic sprouting. Whereas 49 unique candidates were down-regulated, no sufficiently up-regulated candidates were identified at this time point. Several candidates identified in this study are known to influence the translation and degradation of proteins in other systems. The potential role of these factors in the compensatory sprouting of cricket auditory interneurones in response to denervation is discussed. © 2009 The Royal Entomological Society
Discriminating changes in intracellular NADH/NAD+ levels due to anoxicity and H2 supply in R. eutropha cells using the Frex fluorescence sensor
The hydrogen-oxidizing “Knallgas” bacterium Ralstonia eutropha can thrive in aerobic and anaerobic environments and readily switches between heterotrophic and autotrophic metabolism, making it an attractive host for biotechnological applications including the sustainable H2-driven production of hydrocarbons. The soluble hydrogenase (SH), one out of four different [NiFe]-hydrogenases in R. eutropha, mediates H2 oxidation even in the presence of O2, thus providing an ideal model system for biological hydrogen production and utilization. The SH reversibly couples H2 oxidation with the reduction of NAD+ to NADH, thereby enabling the sustainable regeneration of this biotechnologically important nicotinamide cofactor. Thus, understanding the interaction of the SH with the cellular NADH/NAD+ pool is of high interest. Here, we applied the fluorescent biosensor Frex to measure changes in cytoplasmic [NADH] in R. eutropha cells under different gas supply conditions. The results show that Frex is well-suited to distinguish SH-mediated changes in the cytoplasmic redox status from effects of general anaerobiosis of the respiratory chain. Upon H2 supply, the Frex reporter reveals a robust fluorescence response and allows for monitoring rapid changes in cellular [NADH]. Compared to the Peredox fluorescence reporter, Frex displays a diminished NADH affinity, which prevents the saturation of the sensor under typical bacterial [NADH] levels. Thus, Frex is a valuable reporter for on-line monitoring of the [NADH]/[NAD+] redox state in living cells of R. eutropha and other proteobacteria. Based on these results, strategies for a rational optimization of fluorescent NADH sensors are discussed
The Arteriovenous Loop: Engineering of Axially Vascularized Tissue
Background: Most of the current treatment options for large-scale tissue defects represent a serious burden for the patients, are often not satisfying, and can be associated with significant side effects. Although major achievements have already been made in the field of tissue engineering, the clinical translation in case of extensive tissue defects is only in its early stages. The main challenge and reason for the failure of most tissue engineering approaches is the missing vascularization within large-scale transplants. Summary: The arteriovenous (AV) loop model is an in vivo tissue engineering strategy for generating axially vascularized tissues using the own body as a bioreactor. A superficial artery and vein are anastomosed to create an AV loop. This AV loop is placed into an implantation chamber for prevascularization of the chamber inside, e.g., a scaffold, cells, and growth factors. Subsequently, the generated tissue can be transplanted with its vascular axis into the defect site and anastomosed to the local vasculature. Since the blood supply of the growing tissue is based on the AV loop, it will be immediately perfused with blood in the recipient site leading to optimal healing conditions even in the case of poorly vascularized defects. Using this tissue engineering approach, a multitude of different axially vascularized tissues could be generated, such as bone, skeletal or heart muscle, or lymphatic tissues. Upscaling from the small animal AV loop model into a preclinical large animal model could pave the way for the first successful attempt in clinical application. Key Messages: The AV loop model is a powerful tool for the generation of different axially vascularized replacement tissues. Due to minimal donor site morbidity and the possibility to generate patient-specific tissues variable in type and size, this in vivo tissue engineering approach can be considered as a promising alternative therapy to current treatment options of large-scale defects
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