Zelfdoding onder migrantengroepen en autochtonen

Doel. De verschillen vaststellen in incidentie van zelfdoding tussen autochtonen en allochtonen, gerubriceerd naar regio en land van herkomst, rekening houdend met verschillen in leeftijdsopbouw. Opzet. Retrospectief. Methode. Gegevens van de statistiek van de doodsoorzaken van het Centraal Bureau voor de Statistiek (CBS), gebaseerd op de doodsoorzaakverklaringen door arts of lijkschouwer, werden gekoppeld aan het bericht van overlijden uit de gemeentelijke basisadministratie (GBA). Zo kon de herkomst van de overledene worden bepaald op basis van gegevens uit de GBA. De geheimhouding van deze gegevens is wettelijk vastgelegd. Over de jaren 1996-2004 werden alle 13.737 personen geselecteerd die waren overleden door zelfdoding (‘International classification of diseases’(ICD)-10-codes X60-X84). De herkomst werd bepaald aan de hand van gegevens over het geboorteland van de overleden persoon en diens ouders. Iemand is ‘allochtoon’ volgens de definitie van het CBS als ten minste één van de ouders in het buitenland is geboren. Resultaten. Er waren aanzienlijke verschillen in sterfte door zelfdoding tussen migrantengroepen in Nederland. Deze verschillen weerspiegelden deels de suïcidecijfers in de landen en regio’s van herkomst. Migranten uit westerse landen lieten in het algemeen hogere suïcidecijfers zien dan autochtonen. Hoge suïcidecijfers werden waargenomen onder Zuid- en Oost-Europese mannelijke migranten. De suïcidecijfers voor Turken en Marokkanen waren daarentegen significant lager dan die voor autochtonen. Alleen op jongvolwassen leeftijd liepen niet-westerse allochtone mannen, uitgezonderd Marokkanen, een beduidend hoger risico om door zelfdoding te overlijden. Conclusie. Hoewel er veel aandacht is voor pogingen tot suïcide onder Surinaamse vrouwen, waren hun suïcidecijfers minder sterk verhoogd dan die van Surinaamse mannen tot middelbare leeftijd. Mogelijk weerspiegelen deze hoge suïcidecijfers een verhoogd niveau van psychiatrische ziekten, identiteitsproblemen, teleurstellingen in het migratieproces, verwachtingen ten aanzien van opleiding, werk en inkomen, en de verantwoordelijkheid voor gezin en familie

Closing the gap between skills training and academic education at a military academy: An integrated instructional design model

Linking skills training and academic education is a formidable challenge in many professional fields. At modern military academies, officer cadets learn military skills and strategic thinking, fostered by skills training and academic education respectively. As an example, we briefly elaborate on these two learning tracks at the Royal Military Academy of the Netherlands Defence Academy. However, skills training and academic education are often implemented in a non-integrated manner. Because officers have to integrate military skills and strategic thinking during actual military operations, it is paramount that officer cadets learn how to integrate these in a meaningful way. Therefore, we designed an innovative integrated instructional design (ID) model that aims to meet the needs of both military training and academic education. We herein describe the six-step design process of the resulting so-called TrEd ID model, based on the Nine events of instruction model and STAR Legacy, linked through the First Principles of Instruction. The TrEd ID model provides common ground to military instructors and civilian academicians at a military academy, encouraging mutual understanding and collaboration. Future research is needed to understand the potential value of the TrEd ID model in bridging the gap between skills training and academic education, and how to optimally prepare officer cadets for their roles

‘The war is a money making show’: Working-Class Attitudes to World War II and Australian Nationalism

This paper will address the conference themes of ‘class, power and social structure’ through examining industrial and ideological conflict during World War II. The paper will also address the theme of ‘class and culture’ through an examination of working-class cultural expression as a means of resistance to the government’s wartime offensive. What is overlooked in most histories of World War II is the working-class experience of the war and their understanding of nationalism, particularly as nationalism was cynically exploited by the government to undermine working-class identity and solidarity. The paper will investigate the experience of one of the most militant sections of the Australian working class: the Miners. Primary source material such as the Miners’ journal Common Cause and union records reveal opposition to the war and a much more ambiguous attitude to the national sentiment used to justify Australia’s involvement. The Miners provide an interesting case study as the union was led by the Communist Party. Therefore the union leadership initially opposed to the war then became enthusiastic supporters when Russia entered the war on the allied side. It is clear that the Miners’ union leadership found it difficult to convince the rank and file to support the war. The paper will focus upon rank and file attitudes to the war and Australian nationalism particularly during times of industrial unrest.The symposium is organised on behalf of AAHANZBS by the Business and Labour History Group, The University of Sydney, with the financial support of the University’s Faculty of Economics and Business

GATA2 heterozygosity causes an epigenetic feedback mechanism resulting in myeloid and erythroid dysplasia

The transcription factor GATA2 has a pivotal role in haematopoiesis. Heterozygous germline GATA2 mutations result in a syndrome characterized by immunodeficiency, bone marrow failure and predispositions to myelodysplastic syndrome (MDS) and acute myeloid leukaemia. Clinical symptoms in these patients are diverse and mechanisms driving GATA2-related phenotypes are largely unknown. To explore the impact of GATA2 haploinsufficiency on haematopoiesis, we generated a zebrafish model carrying a heterozygous mutation of gata2b (gata2b+/−), an orthologue of GATA2. Morphological analysis revealed myeloid and erythroid dysplasia in gata2b+/− kidney marrow. Because Gata2b could affect both transcription and chromatin accessibility during lineage differentiation, this was assessed by single-cell (sc) RNA-seq and single-nucleus (sn) ATAC-seq. Sn-ATAC-seq showed that the co-accessibility between the transcription start site (TSS) and a −3.5–4.1 kb putative enhancer was more robust in gata2b+/− zebrafish HSPCs compared to wild type, increasing gata2b expression and resulting in higher genome-wide Gata2b motif use in HSPCs. As a result of increased accessibility of the gata2b locus, gata2b+/− chromatin was also more accessible during lineage differentiation. scRNA-seq data revealed myeloid differentiation defects, that is, impaired cell cycle progression, reduced expression of cebpa and cebpb and increased signatures of ribosome biogenesis. These data also revealed a differentiation delay in erythroid progenitors, aberrant proliferative signatures and down-regulation of Gata1a, a master regulator of erythropoiesis, which worsened with age. These findings suggest that cell-intrinsic compensatory mechanisms, needed to obtain normal levels of Gata2b in heterozygous HSPCs to maintain their integrity, result in aberrant lineage differentiation, thereby representing a critical step in the predisposition to MDS.</p

Yap1-Driven Intestinal Repair Is Controlled by Group 3 Innate Lymphoid Cells

Intestinal repair is driven by epithelial stem cells, but how these stem cells are instructed to initiate repair was unknown. Here, Romera-Hernández et al. report that epithelial proliferation after damage is independent of the stem cell-protective signal IL-22 but requires ILC3-dependent amplification of regenerative YAP1 signaling in stem cells.Tissue repair requires temporal control of progenitor cell proliferation and differentiation to replenish damaged cells. In response to acute insult, group 3 innate lymphoid cells (ILC3s) regulate intestinal stem cell maintenance and subsequent tissue repair. ILC3-derived IL-22 is important for stem cell protection, but the mechanisms of ILC3-driven tissue regeneration remain incompletely defined. Here we report that ILC3-driven epithelial proliferation and tissue regeneration are independent of IL-22. In contrast, ILC3s amplify the magnitude of Hippo-Yap1 signaling in intestinal crypt cells, ensuring adequate initiation of tissue repair and preventing excessive pathology. Mechanistically, ILC3-driven tissue repair is Stat3 indepe

Association of HBsAg levels with differential gene expression in NK, CD8 T, and memory B cells in treated patients with chronic HBV

Background &amp; Aims: HBsAg secretion may impact immune responses to chronic HBV infection. Thus, therapeutic approaches to suppress HBsAg production are being investigated. Our study aims to examine the immunomodulatory effects of high and low levels of circulating HBsAg and thereby improve our understanding of anti-HBV immunity. Methods: An optimized 10x Genomics single-cell RNA sequencing workflow was applied to blood samples and liver fine-needle aspirates from 18 patients undergoing tenofovir/entecavir (NUC) treatment for chronic HBV infection. They were categorized based on their HBsAg levels: high (920-12,447 IU/ml) or low (1-100 IU/ml). Cluster frequencies, differential gene expression, and phenotypes were analyzed. Results: In the blood of HBV-infected patients on NUC, the proportion of KLRC2+ “adaptive” natural killer (NK) cells was significantly lower in the HBsAg-high group and, remarkably, both KLRC2+ NK and KLRG1+ CD8 T cells display enrichment of lymphocyte activation-associated gene sets in the HBsAg-low group. High levels of HBsAg were associated with mild immune activation in the liver. However, no suppression of liver-resident CXCR6+ NCAM1+ NK or CXCR6+ CD69+ CD8 T cells was detected, while memory B cells showed signs of activation in both the blood and liver. Conclusions: Among NUC-treated patients, we observed a minimal impact of HBsAg on leukocyte populations in the blood and liver. However, for the first time, we found that HBsAg has distinct effects, restricted to NK-, CD8 T-, and memory B-cell subsets, in the blood and liver. Our findings are highly relevant for current clinical studies evaluating treatment strategies aimed at suppressing HBsAg production and reinvigorating immunity to HBV. Impact and implications: This study provides unique insight into the impact of HBsAg on gene expression levels of immune cell subsets in the blood and liver, particularly in the context of NUC-treated chronic HBV infection. It holds significant relevance for current and future clinical studies evaluating treatment strategies aimed at suppressing HBsAg production and reinvigorating immunity to HBV. Our findings raise questions about the effectiveness of such treatment strategies and challenge the previously hypothesized immunomodulatory effects of HBsAg on immune responses against HBV.</p

The role of the ferric uptake regulator (Fur) in regulation of Helicobacter pylori iron uptake

Background. Availability of the essential nutrient iron is thought to vary greatly in the gastric mucosa, and thus the human gastric pathogen Helicobacter pylori requires regulatory responses to these environmental changes. Bacterial iron-responsive regulation is often mediated by Ferric Uptake Regulator (Fur) homologs, and in this study we have determined the role of H. pylori Fur in regulation of H. pylori iron uptake. Methods. Wild-type H. pylori and fur mutant derivatives were compared after growth in ironrestricted and iron-replete conditions. Iron-uptake was measured using 55Fe-labeled iron, whereas gene expression was mon