Dataset of historic and modern bread and durum wheat cultivar performance under conventional and reduced tillage with full and reduced irrigation

Conservation agriculture (CA) is an agronomic management system based on zero tillage and residue retention. Due to its potential for climate change adaptation through the reduction of soil erosion and improved water availability, CA is becoming more important in many regions of the world. However, increased bulk density and large amounts of crop residues may be a constraint for early plant establishment. This holds especially true under irrigated production areas with high yield potential. Genotype × tillage effects on yield are not well understood and it is unclear whether tillage should be an evaluation factor in breeding programs. Fourteen CIMMYT bread (Triticum aestivum) and thirteen durum (Triticum turgidum) wheat genotypes, created between 1964 and 2011, were tested for yield and agronomic performance at CIMMYT's experimental station near Ciudad Obregon, Mexico, during nine seasons. The genotypes were subjected to different tillage and irrigation treatments which consisted of conventional and permanent raised beds with full and reduced irrigation. The dataset includes traits collected during the growing period (days to emergence, days to flowering, maturity, plant height, NDVI, days from flowering to maturity, grain production rate) and at harvest (yield, harvest index, thousand grain weight, spikes/m², grains/m², test weight) and weather data (daily minimum and maximum temperature, rainfall). Six years of data of 26 genotypes were published along with the Honsdorf et al. (2018) paper in Field Crops Research (DOI: s10.1016/j.fcr.2017.11.011). This updated dataset includes three additional seasons of data (harvest years 2016 to 2018) and an additional bread wheat genotype (Borlaug100)

Extent and structure of linkage disequilibrium in canola quality winter rapeseed (Brassica napus L.)

Linkage disequilibrium was investigated in canola quality winter rapeseed to analyze (1) the prospects for whole-genome association analyses and (2) the impact of the recent breeding history of rapeseed on linkage disequilibrium. A total of 845 mapped AFLP markers with allele frequencies ≥0.1 were used for the analysis of linkage disequilibrium in a population of 85 canola quality winter rapeseed genotypes. A low overall level of linkage disequilibrium was found with a mean r2 of only 0.027 over all 356,590 possible marker pairs. At a significance threshold of P = 2.8 × 10−7, which was derived by a Bonferroni correction from a global α-level of 0.1, only 0.78% of the marker pairs were in significant linkage disequilibrium. Among physically linked marker pairs, the level of linkage disequilibrium was about five times higher with more than 10% of marker pairs in significant linkage disequilibrium. Linkage disequilibrium decayed rapidly with distance between linked markers with high levels of linkage disequilibrium extending only for about 2 cM. Owing to the rapid decay of linkage disequilibrium with distance association analyses in canola quality rapeseed will have a significantly higher resolution than QTL analyses in segregating populations by interval mapping, but much larger number of markers will be necessary to cover the whole genome. A major impact of the recent breeding history of rapeseed on linkage disequilibrium could not be observed

Linking soil microbial community structure to potential carbon mineralization: A continental scale assessment of reduced tillage

Potential carbon mineralization (Cmin) is a commonly used indicator of soil health, with greater Cmin values interpreted as healthier soil. While Cmin values are typically greater in agricultural soils managed with minimal physical disturbance, the mechanisms driving the increases remain poorly understood. This study assessed bacterial and archaeal community structure and potential microbial drivers of Cmin in soils maintained under various degrees of physical disturbance. Potential carbon mineralization, 16S rRNA sequences, and soil characterization data were collected as part of the North American Project to Evaluate Soil Health Measurements (NAPESHM). Results showed that type of cropping system, intensity of physical disturbance, and soil pH influenced microbial sensitivity to physical disturbance. Furthermore, 28% of amplicon sequence variants (ASVs), which were important in modeling Cmin, were enriched under soils managed with minimal physical disturbance. Sequences identified as enriched under minimal disturbance and important for modeling Cmin, were linked to organisms which could produce extracellular polymeric substances and contained metabolic strategies suited for tolerating environmental stressors. Understanding how physical disturbance shapes microbial communities across climates and inherent soil properties and drives changes in Cmin provides the context necessary to evaluate management impacts on standardized measures of soil microbial activity

QTL controlling grain filling under terminal drought stress in a set of wild barley introgression lines.

Drought is a major abiotic stress impeding the yield of cereal crops globally. Particularly in Mediterranean environments, water becomes a limiting factor during the reproductive developmental stage, causing yield losses. The wild progenitor of cultivated barley Hordeum vulgare ssp spontaneum (Hsp) is a potentially useful source of drought tolerance alleles. Wild barley introgression lines like the S42IL library may facilitate the introduction of favorable exotic alleles into breeding material. The complete set of 83 S42ILs was genotyped with the barley 9k iSelect platform in order to complete genetic information obtained in previous studies. The new map comprises 2,487 SNPs, spanning 989.8 cM and covering 94.5% of the Hsp genome. Extent and positions of introgressions were confirmed and new information for ten additional S42ILs was collected. A subset of 49 S42ILs was evaluated for drought response in four greenhouse experiments. Plants were grown under well-watered conditions until ten days post anthesis. Subsequently drought treatment was applied by reducing the available water. Several morphological and harvest parameters were evaluated. Under drought treatment, trait performance was reduced. However, there was no interaction effect between genotype and treatment, indicating that genotypes, which performed best under control treatment, also performed best under drought treatment. In total, 40 QTL for seven traits were detected in this study. For instance, favorable Hsp effects were found for thousand grain weight (TGW) and number of grains per ear under drought stress. In particular, line S42IL-121 is a promising candidate for breeding improved malting cultivars, displaying a TGW, which was increased by 17% under terminal drought stress due to the presence of an unknown wild barley QTL allele on chromosome 4H. The introgression line showed a similar advantage in previous field experiments and in greenhouse experiments under early drought stress. We, thus, recommend using S42IL-121 in barley breeding programs to enhance terminal drought tolerance

Genetic characterization of position and extent of <i>Hsp</i> introgressions of 83 S42ILs based on the Infinium 9k iSelect assay.

<p>Genetic characterization of position and extent of <i>Hsp</i> introgressions of 83 S42ILs based on the Infinium 9k iSelect assay.</p

Genetic map with selected S42IL introgressions, QTL are placed right to the S42ILs, indicated by trait abbreviations (see Table 1).

<p>The sign indicates an increasing (+) or decreasing (-) <i>Hsp</i> effect.</p

Map of 83 S42ILs containing 3,059 SNP markers.

<p>Map of 83 S42ILs containing 3,059 SNP markers.</p

List of S42ILs significantly different from Scarlett under drought stress for each trait.

<p>List of S42ILs significantly different from Scarlett under drought stress for each trait.</p

High-throughput phenotyping to detect drought tolerance QTL in wild barley introgression lines.

Drought is one of the most severe stresses, endangering crop yields worldwide. In order to select drought tolerant genotypes, access to exotic germplasm and efficient phenotyping protocols are needed. In this study the high-throughput phenotyping platform "The Plant Accelerator", Adelaide, Australia, was used to screen a set of 47 juvenile (six week old) wild barley introgression lines (S42ILs) for drought stress responses. The kinetics of growth development was evaluated under early drought stress and well watered treatments. High correlation (r=0.98) between image based biomass estimates and actual biomass was demonstrated, and the suitability of the system to accurately and non-destructively estimate biomass was validated. Subsequently, quantitative trait loci (QTL) were located, which contributed to the genetic control of growth under drought stress. In total, 44 QTL for eleven out of 14 investigated traits were mapped, which for example controlled growth rate and water use efficiency. The correspondence of those QTL with QTL previously identified in field trials is shown. For instance, six out of eight QTL controlling plant height were also found in previous field and glasshouse studies with the same introgression lines. This indicates that phenotyping juvenile plants may assist in predicting adult plant performance. In addition, favorable wild barley alleles for growth and biomass parameters were detected, for instance, a QTL that increased biomass by approximately 36%. In particular, introgression line S42IL-121 revealed improved growth under drought stress compared to the control Scarlett. The introgression line showed a similar behavior in previous field experiments, indicating that S42IL-121 may be an attractive donor for breeding of drought tolerant barley cultivars

Descriptive statistics for traits measures in S42IL population per treatment.

<p>Descriptive statistics for traits measures in S42IL population per treatment.</p