Isolation and culture of murine bone marrow-derived macrophages for nitric oxide and redox biology

Macrophages are mononuclear phagocytes derived from haematopoietic progenitors that are widely distributed throughout the body. These cells participate in both innate and adaptive immune responses and lie central to the processes of inflammation, development, and homeostasis. Macrophage physiology varies depending on the environment in which they reside and they exhibit rapid functional adaption in response to external stimuli. To study macrophages in vitro, cells are typically cultured ex vivo from the peritoneum or alveoli, or differentiated from myeloid bone marrow progenitor cells to form bone marrow-derived macrophages (BMDMs). BMDMs represent an efficient and cost-effective means of studying macrophage biology. However, the inherent sensitivity of macrophages to biochemical stimuli (such as cytokines, metabolic intermediates, and RNS/ROS) makes it imperative to control experimental conditions rigorously. Therefore, the aim of this study was to establish an optimised and standardised method for the isolation and culture of BMDMs. We used classically activated macrophages isolated from WT and nitric oxide (NO)-deficient mice to develop a standardised culture method, whereby the constituents of the culture media are defined. We then methodically compared our standardised protocol to the most commonly used method of BMDM culture to establish an optimal protocol for the study of nitric oxide (NO)-redox biology and immunometabolism in vitro. [Abstract copyright: Copyright © 2020. Published by Elsevier Inc.

Reliability of a new virtual reality test to measure cervicocephalic kinaesthesia

The aim of this study was to investigate the cervicocephalic kinaesthesia of healthy subjects for gender and age effects and its reliability in a new virtual reality test procedure. 57 healthy subjects (30 male, 27 females; 18-64 years) were immersed into a virtual 3D scene via a headmounted display, which generated specific head movements. The joint repositioning error was determined in a static and dynamic test at the times T0, T1 (T0 + 10 minutes) and T2 (T0 + 24 hours). The intrasession reliability (T0-T1) and the intersession reliability (T0-T2) were analysed. In both tests no gender- or age-specific effects were found. In the overall group the means of the static test were 6.2 - 6.9 and of the dynamic test were 4.5 -4.9 . The intratest difference in the static test was -0.16 and the intertest difference was 0.47 . The intratest difference in the dynamic test was 0.42 and the intertest difference was 0.37 . The static and dynamic test was reproducible in healthy subjects, with minor deviations, irrespective of gender and age. The smaller interindividual differences in the dynamic test could be beneficial in the comparison of healthy individuals and individuals with cervical spine disorders

Beyond green : Broad support for biodiversity in multicultural European cities

While urban growth contributes to the biodiversity crisis, biodiverse greenspaces within cities could support both human wellbeing and biodiversity conservation. Yet, urban greenspaces are under pressure due to the rapid densification of cities worldwide. Urban conservation policies thus need broad support, ideally from people with different sociocultural backgrounds. Whether urban residents prefer biodiverse over simply green spaces, however, largely remains an open question. We tested how diverse respondents (N = 3716) from five European cities valued three levels of biodiversity (plant species richness) in four ubiquitous greenspace types. Our field survey revealed that biodiversity matters: People largely prefer higher plant species richness in urban greenspaces (i.e., parks, wastelands, streetscapes) and agree that higher plant species richness allows for more liveable cities. Despite variation across European cities, positive valuations of high plant species richness prevailed among different sociocultural groups, including people of migrant background. The results of this study can thus support policies on a biodiversity-friendly development and management of urban greenspaces by highlighting social arguments for integrating biodiversity into urban development plans

Beyond green : Broad support for biodiversity in multicultural European cities

While urban growth contributes to the biodiversity crisis, biodiverse greenspaces within cities could support both human wellbeing and biodiversity conservation. Yet, urban greenspaces are under pressure due to the rapid densification of cities worldwide. Urban conservation policies thus need broad support, ideally from people with different sociocultural backgrounds. Whether urban residents prefer biodiverse over simply green spaces, however, largely remains an open question. We tested how diverse respondents (N = 3716) from five European cities valued three levels of biodiversity (plant species richness) in four ubiquitous greenspace types. Our field survey revealed that biodiversity matters: People largely prefer higher plant species richness in urban greenspaces (i.e., parks, wastelands, streetscapes) and agree that higher plant species richness allows for more liveable cities. Despite variation across European cities, positive valuations of high plant species richness prevailed among different sociocultural groups, including people of migrant background. The results of this study can thus support policies on a biodiversity-friendly development and management of urban greenspaces by highlighting social arguments for integrating biodiversity into urban development plans

Beyond green: Broad support for biodiversity in multicultural European cities

While urban growth contributes to the biodiversity crisis, biodiverse greenspaces within cities could support both human wellbeing and biodiversity conservation. Yet, urban greenspaces are under pressure due to the rapid densification of cities worldwide. Urban conservation policies thus need broad support, ideally from people with different sociocultural backgrounds. Whether urban residents prefer biodiverse over simply green spaces, however, largely remains an open question. We tested how diverse respondents (N = 3716) from five European cities valued three levels of biodiversity (plant species richness) in four ubiquitous greenspace types. Our field survey revealed that biodiversity matters: People largely prefer higher plant species richness in urban greenspaces (i.e., parks, wastelands, streetscapes) and agree that higher plant species richness allows for more liveable cities. Despite variation across European cities, positive valuations of high plant species richness prevailed among different sociocultural groups, including people of migrant background. The results of this study can thus support policies on a biodiversity-friendly development and management of urban greenspaces by highlighting social arguments for integrating biodiversity into urban development plans

Beyond green: broad support for biodiverse cities in Europe

While urban growth contributes to the biodiversity crisis, biodiverse greenspaces within cities could support both human wellbeing and biodiversity conservation. Yet, urban greenspaces are under pressure due to the rapid densification of cities worldwide. Urban conservation policies thus need broad support, ideally from people with different sociocultural backgrounds. Whether urban residents prefer biodiverse over simply green spaces, however, largely remains an open question. We tested how diverse respondents (N = 3716) from five European cities valued three levels of biodiversity (plant species richness) in four ubiquitous greenspace types. Our field survey revealed that biodiversity matters: People largely prefer higher plant species richness in urban greenspaces (i.e., parks, wastelands, streetscapes) and agree that higher plant species richness allows for more liveable cities. Despite variation across European cities, positive valuations of high plant species richness prevailed among different sociocultural groups, including people of migrant background. The results of this study can thus support policies on a biodiversity-friendly development and management of urban greenspaces by highlighting social arguments for integrating biodiversity into urban development plans