A recombinant avian antibody against VP2 of infectious bursal disease virus protects chicken from viral infection

【Abstract】A stable cell-line was established that expressed the recombinant avian antibody (rAb) against the infectious bursal disease virus (IBDV). rAb exhibited neutralization activity to IBDV-B87 strain in DF1 cells. The minimum rAb concentration required for inhibition of the cytopathic effect (CPE) was 1.563 μg/mL. To test the efficacy of rAb, a 168-h cohabitation challenge experiment was performed to transmit the disease from the chickens challenged with vvIBDV (HLJ0504 strain) to three test groups of chickens, i.e. (1) chickens treated with rAb, (2) chickens treated with yolk antibody, and (3) non-treatment chickens. The survival rates of chickens treated with rAb, yolk antibody and without treatment were 73%, 67% and 20%, respectively. Another batch of chickens was challenged with IBDV (BC6/85 strain) and then injected with rAb (1.0 mg/kg) 6, 24 and 36 h post-challenge. Non-treatment chickens had 100% morbidity, whereas those administered with rAb exhibited only 20% morbidity. Morbidity was evaluated using clinical indicators and bursal histopathological section. This study provides a new approach to treating IBDV and the rAb represents a promising candidate for this IBDV therapy.This research was supported by Heilongjiang province project of applied technology research and development (2013GC13C105) and The National Natural Science Fund biologic science base improve program of research training and capacity (J1210069/J0124)

The genetic and epigenetic regulation of CD55 and its pathway analysis in colon cancer

BackgroundCD55 plays an important role in the development of colon cancer. This study aims to evaluate the expression of CD55 in colon cancer and discover how it is regulated by transcriptional factors and miRNA.MethodsThe expression of CD55 was explored by TIMER2.0, UALCAN, and Human Protein Atlas (HPA) databases. TRANSFAC and Contra v3 were used to predict the potential binding sites of transcription factors in the CD55 promoter. TargetScan and starBase v2.0 were used to predict the potential binding ability of miRNAs to the 3′ untranslated region (3′UTR) of CD55. SurvivalMeth was used to explore the differentially methylated sites in the CD55 promoter. Western blotting was used to detect the expression of TFCP2 and CD55. Dual-luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay were performed to determine the targeting relationship of TFCP2, NF-κB, or miR-27a-3p with CD55. CD55-related genes were explored by constructing a protein–protein interaction (PPI) network and performing pathway analysis by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).ResultsCD55 was highly expressed in colon cancer tissues. The mRNA and protein expression levels of TFCP2 were reduced by si-TFCP2. NF-κB mRNA was obviously reduced by NF-κB inhibitor and increased by NF-κB activator. CD55 protein was also inhibited by miR-27a-3p. Dual-luciferase reporter assays showed that after knocking down TFCP2 or inhibiting NF-κB, the promoter activity of CD55 was decreased by 21% and 70%, respectively; after activating NF-κB, the promoter activity of CD55 increased by 2.3 times. As TFCP2 or NF-κB binding site was mutated, the transcriptional activity of CD55 was significantly decreased. ChIP assay showed that TFCP2 and NF-κB combined to the promoter of CD55. The luciferase activity of CD55 3′UTR decreased after being co-transfected with miR-27a-3p mimics and increased by miR-27a-3p antagomir. As the miR-27a-3p binding site was mutated, we did not find any significant effect of miR-27a-3p on reporter activity. PPI network assay revealed a set of CD55-related genes, which included CFP, CFB, C4A, and C4B. GO and KEGG analyses revealed that the target genes occur more frequently in immune-related pathways.ConclusionOur results indicated that CD55 is regulated by TFCP2, NF-κB, miR-27a-3p, and several immune-related genes, which in turn affects colon cancer

Comprehensive Analysis of the Relationship Between RAS and RAF Mutations and MSI Status of Colorectal Cancer in Northeastern China

Background/Aims: Colorectal cancer (CRC) is mainly caused by chromosomal instability (CIN) and microsatellite instability (MSI). The RAS and RAF genes are essential components of the CIN pathway, and several studies have found that RAS and RAF mutations are associated with MSI status in CRC. Here, we examined these three factors in CRC in Northeast China and aimed to reveal new details of the relationship between these mutations and MSI status. Methods: This study involved 290 patients with CRC who had RAS or RAF gene mutation detected using fluorescence-based allele-specific polymerase chain reaction or Sanger sequencing. The majority of the identified patients were found to harbor MSI (MSI status). Accurate molecular detection was carried out using formalin-fixed paraffin-embedded tissue or blood samples. Results: The rates of RAS and RAF mutations were 58.5% and 4.1%, respectively. The prevalence of RAS mutation in CRC was clearly higher and that of RAF mutation was lower in Northeast China compared with previously reported cohorts in other locations. High MSI level (MSI-H status) was more complex, at around 10%. This was consistent with previous data from China. However, compared with data reported from other continents, MSI-H was higher than that of Japan or South Korea in Asia, and lower than that of Europe or the United States. Conclusion: RAS/RAF mutations and MSI status in CRC are closely associated with tumor location and ethnicity. Further studies investigating the relationship between these three factors can help in the development of treatment strategies for patients with CRC

Runoff simulation analysis and collaborative response research based on the second Songhua River basin under the background of land use

This paper examines the context of climate change and land use in the second Songhua River basin with the goal of improving runoff. The report begins by introducing the history of watershed runoff and the study area. The differences between various land use types and land use efficiency are examined using the watershed hydrology model from previous literature. The novelty of the paper is to compare the evapotranspiration of the model in different periods with the depth data of surface runoff. The results show that the simulation analysis and collaborative response strategy proposed here can adapt to the meteorological changes in the basin. The evapotranspiration of a watershed that was converted from woodland to grassland in 1970 was 34 mm, while that of a watershed that was converted from grassland to woodland was 32 mm, according to the results of the model test. The evapotranspiration of a watershed that gone from woodland to grassland in 2010 is 45 mm, compared to 39 mm for a watershed that has gone from grassland to woodland. The second Songhua River basin's surface water yield data can therefore be used to model and study the basin's runoff in real time. HIGHLIGHTS The overall change of runoff in the second Songhua River basin from 1970 to 2020 shows a certain growth trend.; The evapotranspiration of the second Songhua River basin fluctuated greatly from 1970 to 2020.; A runoff simulation and collaborative response model was constructed.

Effects of Different Propagation Materials and Cultivation Years on the Yield of Polygonatum odoratum under the Forest

This paper mainly studies the effects of different propagation materials and cultivation years on the yield of Polygonatum odoratum under the forest. In this study, the cultivation experiment of P. odoratum was carried out by using the stem tip, stem body and seeds of P. odoratum as propagation materials. The yield of P.odoratum in different cultivation years with propagation by rhizome was also investigated. That is, the continuous growth of P. odoratum and the yield of P. odoratum each year after cultivation was statistically analyzed. The results showed that the germination rate under stem tip cultivation was fast, and the emergence time was also fast, which was more than 2 weeks earlier than that under stem body cultivation, and the seedling emergence rate reached 100%. The stem body was a new plant formed by sprouting from the buds of internodes, and the emergence rate was more than 95%. P. odoratum propagated by rhizome was harvested in the third or fourth year after cultivation, and the third year was the best harvest time

Effects of N, N-Dimethylformamide on Infertility and Palatability of Quinestrol

【Objective】The study was carried out to clear and definite that the addition of efficient and safe auxiliary N, N-Dimethylformamide (DMF) can improve the sterility of quinestrol baits, and improving the palatability of quinestrol baits to mice.【Method】The mice were divided into three groups C, T1 and T2 fed with blank bait, quinestrol bait and DMF-quinestrol bait, respectively, and the bait consumption was calculated. The male and female mice of three treatment groups were caged for 7 days, and the pregnancy and delivery of the female mice were recorded. The high-performance liquid chromatography used to detection the content of quinestrol in brown rice in the different formulations of baits.【Result】Both the T1 and T2 groups had significant effects on the body weight of male and female mice. The wet weights of the kidney organs in the T1 and T2 groups were 16.4% and 19.7% lower than the C group, respectively. Quinestrol had significant inhibitory effect on the reproduction of female mice, with the reproductive rate of female mice in T1 and T2 groups decreased to 37.5% and 25.0% of group C, and the number of litters was only 35.0% and 11.1% of group C, respectively. DMF did not significantly improve the palatability of quinestrol drug bait, but the results of high-performance liquid chromatography showed that the content of quinestrol in brown rice in the T2 group was 1.5 times higher than that in the T1 group. DMF increased the permeability of quinestrol in brown rice, thereby increasing the drug intake of mice.【Conclusion】This study shows that DMF can effectively dissolve quinestrol and enhance its permeability in brown rice, improving the contraceptive effect of quinestrol drug bait, and has the potential to be developed into a promising contraceptive formulation

Optimization of Cancer Risk Assessment Models for PM2.5-Bound PAHs: Application in Jingzhong, Shanxi, China

The accurate evaluation of the carcinogenic risk of PM2.5-bound polycyclic aromatic hydrocarbons (PAHs) is crucial because of the teratogenic, carcinogenic, and mutagenic effects of PAHs. The best model out of six models was selected across three highly used categories in recent years, including the USEPA-recommended inhalation risk (Model I), inhalation carcinogen unit risk (Models IIA–IID), and three exposure pathways (inhalation, dermal, and oral) (Model III). Model I was found to be superior to the other models, and its predicted risk values were in accordance with the thresholds of PM2.5 and benzo[a]pyrene in ambient-air-quality standards. Models IIA and III overestimated the risk of cancer compared to the actual cancer incidence in the local population. Model IID can replace Models IIB and IIC as these models exhibited no statistically significant differences between each other. Furthermore, the exposure parameters were optimized for Model I and significant differences were observed with respect to country and age. However, the gender difference was not statistically significant. In conclusion, Model I is recommended as the more suitable model, but in assessing cancer risk in the future, the exposure parameters must be appropriate for each country