Jüdische Friedhöfe und Bestattungskultur in Europa

Jüdische Friedhöfe und Bestattungskultur in Europa, ICOMOS Hefte des Deutschen Nationalkomitees LIII, hendrik Bäßler verlag, Berlin 2011. Rezensiert von Jan Trenne

Biodegradation kinetics of MC-YR catalyzed by CEs of USTB-05.

<p>Biodegradation kinetics of MC-YR catalyzed by CEs of USTB-05.</p

Liquid chromatogram-mass spectrum (LC-MS) protonated molecular ion for product B.

<p>Liquid chromatogram-mass spectrum (LC-MS) protonated molecular ion for product B.</p

Liquid chromatogram-mass spectrum (LC-MS) protonated molecular ion for MC-YR.

<p>Liquid chromatogram-mass spectrum (LC-MS) protonated molecular ion for MC-YR.</p

Proposed biodegradation pathway of MC-YR by Sphingopyxis sp. USTB-05.

<p>Proposed biodegradation pathway of MC-YR by Sphingopyxis sp. USTB-05.</p

Liquid chromatogram-mass spectrum (LC-MS) profile of product A.

<p>Liquid chromatogram-mass spectrum (LC-MS) profile of product A.</p

Liquid chromatogram-mass spectrum (LC-MS) profile of MC-YR.

<p>Liquid chromatogram-mass spectrum (LC-MS) profile of MC-YR.</p

Synergistic Coupling of Fluorescent “Turn-Off” with Spectral Overlap Modulated FRET for Ratiometric Ag⁺ Sensor

A useful strategy for ratiometric fluorescent detecting of Ag⁺ is demonstrated. Upon selective binding of Ag⁺ to a BODIPY-porphyrin dyad (<b>1</b>), the synergistic coupling of two functions, namely the suppressing of FRET from BODIPY donor to porphyrin acceptor and the fluorescence quenching of porphyrin acceptor, leads to exceptionally large changes in the intensity ratio of two distinct emissions (<i>F</i>₅₁₃<i>/F</i>₆₅₄) which allow for the ratiometric detecting of Ag⁺ with excellent sensitivity in solution and living cells

Anti-PHB autoantibodies induced in IgG4-RD patients.

<p>(A) The prevalence of autoantibodies against human PHB in sera from patients was observed. ELISA was used to detect the reactivity of serum IgG4 against recombinant human PHB protein. The anti-PHB antibodies were detected in 65 of 89 RA patients (73%), 4 of 30 SjS patients (13.3%) and 1 of 70 healthy donors (1.4%). The reactivity of anti-PHB antibodies was significantly higher than HC (***<i>P</i><0.0001). (B) The patients with IgG4-RD were then divided into the following confirmed subtypes: AIP, definite autoimmune pancreatitis (25/34, 73.5%); MD, Mikulicz’s disease (8/15, 53.3%); RPF, retroperitoneal fibrosis (6/11, 54.5%); MIX, affect multiply organs (26/29, 89.7%).</p

Verification of prohibitin.

<p>(A) The cloning, expression and purification of recombinant PHB protein. M, protein markers; lane 1, cell extracts of pET-28a (+)-PHB/BL21 after IPTG induction for 6 hour at 37°C; lane 2, cell extracts of pET-28a (+)-PHB/BL21 before IPTG induction; lane 3, cell extracts of pET-28a (+)-BL21 after IPTG induction. lane 4, cell extracts of BL21 after IPTG induction. (B) Western blot using purified PHB protein showed that only the sera from patients with IgG4-RD (lane 1) rather than HC (lane 2) contain antibodies against a 30 kDa cellular protein. (C) The expressed protein was purified and further identified by MS, which revealed its identity as PHB. (D) PHB protein was also identified in immunoprecipitates; lane 1, supernatant of immunoprecipitation; lane 2, immunoprecipitates; lane 3, control sample (the purified rhPHB). (E) The protein band on lane 2 was excised and identified by MALDI-TOF/TOF MS, which again revealed its identity as PHB.</p