Physiological, proteomic and metabolomic analysis provide insights into Ca2+ tolerance in Drynaria roosii leaves

The specific mechanisms by which Ca2+ affects vegetative growth of plants in karst area are still unclear. The aim of the current experiment was to study the effects of excessive Ca2+ on physiological characteristics, proteins and metabolites of leaves of two-year old Drynaria roosii for 14 days and provide a theoretical reference for analyzing the mechanisms of the interaction between high concentration Ca2+ and vegetative growth of plants in karst area. We sought to investigate the effect of 0 mM Ca2+, 600 mM Ca2+, and 1200 mM Ca2+ on the properties of leaves of D. roosii. Physiological analysis reveals that excessive Ca2+ might inhibit the growth of D. roosii leaves by osmotic stress. Proteomics analysis suggests that differentially expressed proteins mainly involved in protein metabolism, amino acid metabolism, carbohydrate and energy metabolism, photosynthesis, antioxidant and defense. Metabolomics analysis indicates that differentially expressed metabolites mainly involved in amino acid metabolism, lignin biosynthesis, and flavonoid biosynthesis. These results give insights into the biological processes apparently involved in Ca2+ tolerance in this species

Research Progress on Solidification of Soft Muddy Soil

A large number of literatures which had reported soft muddy soil stabilization were reviewed to review the present status and classification of soft muddy soil solidifying materials, summarize and analyze the solidifying mechanism of various solidifying materials in this paper. The results show that the soft muddy soil solidification which has significant economic, environmental and social benefit has wide application potential. Meanwhile, the questions need to be solved at present and suggestions of soft muddy soil solidification have been put forward

Research Progress on Solidification of Soft Muddy Soil

A large number of literatures which had reported soft muddy soil stabilization were reviewed to review the present status and classification of soft muddy soil solidifying materials, summarize and analyze the solidifying mechanism of various solidifying materials in this paper. The results show that the soft muddy soil solidification which has significant economic, environmental and social benefit has wide application potential. Meanwhile, the questions need to be solved at present and suggestions of soft muddy soil solidification have been put forward

Biosorption Mechanism of Aqueous Pb2+, Cd2+, and Ni2+ Ions on Extracellular Polymeric Substances (EPS)

Heavy metal pollution has been a focus with increasing attention, especially Pb2+, Cd2+, and Ni2+ in an aqueous environment. The adsorption capacity and mechanism of extracellular polymeric substances (EPS) from Agrobacterium tumefaciens F2 for three heavy metals were investigated in this study. The adsorption efficiency of 94.67%, 94.41%, and 77.95% were achieved for Pb2+, Cd2+, and Ni2+ adsorption on EPS, respectively. The experimental data of adsorption could be well fitted by Langmuir, Freundlich, Dubinin–Radushkevich isotherm models, and pseudo-second-order kinetic model. Model parameters analysis demonstrated the great adsorption efficiency of EPS, especially for Pb2+, and chemisorption was the rate-limiting step during the adsorption process. The functional groups of C=O of carboxyl and C-O-C from sugar derivatives in EPS played the major role in the adsorption process judged by FTIR. In addition, 3D-EEM spectra indicated that tyrosine also assisted EPS adsorption for three heavy metals. But EPS from strain F2 used the almost identical adsorption mechanism for three kinds of divalent ions of heavy metals, so the adsorption efficiency difference of Pb2+, Cd2+, and Ni2+ on EPS could be correlated to the inherent characteristics of each heavy metal. This study gave the evidence that EPS has a great application potential as a bioadsorbent in the treatment of heavy metals pollution

MOESM1 of TP53TG1 enhances cisplatin sensitivity of non-small cell lung cancer cells through regulating miR-18a/PTEN axis

Additional file 1: Figure S1. PTEN expression in NSCLC was analyzed using the TCGA dataset. Red represents NSCLC tumor tissue (n = 483), and black represents normal tissues (n = 347)