Indole-thiophene conjugate inhibits proliferation of human cervical cancer cell lines through DNA damage

Purpose: To investigate the inhibitory effect of indole-thiophene conjugate (ITC) against cervical cancer cells. Methods: The effect of ITC on the proliferation of cervical cells was determined using 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) assay. The apoptosis-inducing effect of ITC was analysed with flow cytometry, while its effect on cell invasion was assessed using Transwell assay. Results: ITC inhibited proliferation of HeLa and Caski cancer cell lines, but it had no cytotoxicity against HCvEpC normal epithelial cells. Exposure to ITC at a dose of 12 μmol/L reduced the viability of HeLa and Caski cells to 22.56 and 24.78 %, respectively (p < 0.05). ITC treatment of HeLa cells enhanced the proportion of apoptotic cells. Exposure to ITC at a dose of 12 μmol/L led to near-complete inhibition of the invasive potential of HeLa cells. Moreover, exposure of HeLa cells to ITC downregulated the protein expressions of MMP-2 and MMP-9 (p < 0.05). The expressions of Bcl-2, p-ERK1/2 and p-Akt were markedly decreased in HeLa cells by ITC exposure. In addition, ITC increased Bax expression, and decreased Bcl-2/Bax ratio (p < 0.05). Conclusion: ICT inhibits the proliferation and invasion of cervical cancer cells, and induces their apoptosis. It exhibits these effects via the suppression of Akt and ERK phosphorylation, thereby downregulating the PI3K and MAPK pathways. Therefore, ITC may be beneficial for the treatment of cervical cancer

Numerical analysis on magnetic leakage field of pipeline defect

Pipeline magnetic flux leakage inspection, mainly used for pipeline defect detection, is an important means of inner examination technology on pipeline. Flux leakage testing can't obtain valid defect identification signals by one hundred percent because of magnetization of the magnetic leakage field, the measured shape, size and location of pipeline defects, materials and operating conditions, and lift-off value, pole pitch and the length of steel brush during the measurement as well as forged pipe fittings such as welding seam, stiffener, flange and tee on the pipeline to be tested. This article reaches a conclusion that magnetic flux density distribution is influenced by the depth and width of defect through respectively researching magnetic leakage field of individual defect and double defects (thickness type) by finite-element method. It also conducts the numerical analysis on pipeline welding seam, stiffener, flange (increased wall thickness type) and tee (compound) leakage magnetic field in detection conditions of the same direction, and concludes their distribution rules of magnetic flux density. The characteristic parameters of distinguishing defect magnetic flux leakage field and the part of the pipeline magnetic flux leakage, derived from analysis and comparison of results on defective pipeline and conduit joint, stiffener, flange and tee magnetic flux leakage, provide a foundation of qualitative identification for accurately recognizing pipeline defect and eliminating the impact of other ancillary fittings on a pipe on pipeline magnetic flux leakage, and they can also offer infallible data to pipeline maintenance as a basis of quantitative analysis

Numerical investigation of the energy performance of a guideless irregular heat and mass exchanger with corrugated heat transfer surface for dew point cooling

© 2016 The Author(s) The paper presents an investigation into the energy performance of a novel irregular heat and mass exchanger for dew point cooling which, compared to the existing flat-plate heat exchangers, removed the use of the channel supporting guides and implemented the corrugated heat transfer surface, thus expecting to achieve the reduced air flow resistance, increased heat transfer area, and improved energy efficiency (i.e. Coefficient of Performance (COP)) of the air cooling process. CFD simulation was carried out to determine the flow resistance (K) factors of various elements within the dry and wet channels of the exchanger, while the ‘finite-element’ based ‘Newton-iteration’ numerical simulation was undertaken to investigate its cooling capacity, cooling effectiveness and COP at various geometrical and operational conditions. Compared to the existing flat-plate heat and mass exchangers with the same geometrical dimensions and operational conditions, the new irregular exchanger could achieve 32.9%–37% higher cooling capacity, dew-point and wet-bulb effectiveness, 29.7%–33.3% higher COP, and 55.8%–56.2% lower pressure drop. While undertaking dew point air cooling, the irregular heat and mass exchanger had the optimum air velocity of 1 m/s within the flow channels and working-to-intake air ratio of 0.3, which allowed the highest cooling capacity and COP to be achieved. In terms of the exchanger dimensions, the optimum height of the channel was 5 mm while its length was in the range 1–2 m. Overall, the proposed irregular heat and mass exchanger could lead to significant enhanced energy performance compared to the existing flat-plate dew point cooling heat exchanger of the same geometrical dimensions. To achieve the same amount cooling output, the irregular heat and mass exchanger had the reduced size and cost against the flat-plate ones

Shear slitting of aluminum webs using block knives

C Shear slitting of two aluminum webs, namely 1050 H18 of 0.28 mm thick and 5182 H19 of 0.20 mm thick, using block knives are investigated through experiments using a laboratory slitter. This investigation focused on two aspects of shear slitting using block knives, appropriate for relatively thick webs. They are: (1) tangential shear slitting at zero rake angle, i.e., traditional shear slitting with a pair of block knives. In this aspect, the effects of major slitting parameters on the burr height at the slit edge were investigated. These include the clearance, overlap, overdrive and cant angle. The critical clearances for both webs have been determined; and (2) slitting at a rake angle, a new method for edge trimming when the two blades are not necessarily in contact. The top blade geometry was modified for slitting with a rake angle of -15o to allow slitting of an aluminum web, up to 1 mm thick in this investigation. This new method of edge trimming using block knives was found to be very effective and robust over a (relatively) very wide range of slitting parameters. Very good slit edge was produced, and the burr height was found to be independent of slitting parameters over a relatively large range of slitting parameters. Because two blades do not have to be in contact in slitting so that the blade wear is much less than in the case of traditional shear slitting, this new method is expected to extend significantly the block knife service life while producing consistently high quality slit edges.Mechanical and Aerospace Engineerin

A room-temperature electrical-field-enhanced ultrafast switch in organic microcavity polariton condensates

Integrated electro-optical switches are essential as one of the fundamental elements in the development of modern optoelectronics. As an architecture for photonic systems, exciton polaritons, that are hybrid bosonic quasiparticles that possess unique properties derived from both excitons and photons, have shown much promise. For this system, we demonstrate a significant improvement of emitted intensity and condensation threshold by applying an electric field to a microcavity filled with an organic microbelt. Our theoretical investigations indicate that the electric field makes the excitons dipolar and induces an enhancement of the exciton-polariton interaction and of the polariton lifetime. Based on these electric field induced changes, a sub-nanosecond electrical-field-enhanced polariton condensate switch is realized at room temperature, providing the basis for developing an on-chip integrated photonic device in the strong light-matter coupling regime

A versatile route to fabricate single atom catalysts with high chemoselectivity

Preparation of single atom catalysts (SACs) is of broad interest to materials scientists and chemists but remains a formidable challenge. Herein, we develop an efficient approach to synthesize SACs via a precursor-dilution strategy, in which metalloporphyrin (MTPP) with target metals are co-polymerized with diluents (tetraphenylporphyrin, TPP), followed by pyrolysis to N-doped porous carbon supported SACs (M1/N-C). Twenty-four different SACs, including noble metals and non-noble metals, are successfully prepared. In addition, the synthesis of a series of catalysts with different surface atom densities, bi-metallic sites, and metal aggregation states are achieved. This approach shows remarkable adjustability and generality, providing sufficient freedom to design catalysts at atomic-scale and explore the unique catalytic properties of SACs. As an example, we show that the prepared Pt1/N-C exhibits superior chemoselectivity and regioselectivity in hydrogenation. It only converts terminal alkynes to alkenes while keeping other reducible functional groups such as alkenyl, nitro group, and even internal alkyne intact