3-(3-Bromo­phen­yl)-N-phenyl­oxirane-2-carboxamide

There are two independent mol­ecules in the asymmetric unit of the title compound, C15H12BrNO2. In both mol­ecules, the two benzene rings adopt a cis configuration with respect to the ep­oxy ring. In one mol­ecule, the ep­oxy ring makes dihedral angles of 60.5 (2) and 77.92 (19)° with the two benzene rings; in the other mol­ecule, the values are 61.0 (2) and 81.43 (19)°. Inter­molecular N—H⋯O and C—H⋯O hydrogen bonding is present in the crystal structure

Dynamic changes of main metabolic substances during anther-derived embryos development in loquat (Eriobotrya japonica Lindl. cv. ‘Dawuxing’)

The main metabolic substances changes during the development process of anther-derived embryos in loquat (Eriobotrya japonica Lindl. cv. ‘Dawuxing’) were studied. These include water contents, dry mass contents, carbohydrates, soluble proteins and nucleic acids. In the developmental stages of anther-derived embryos, the fresh weight and the dry mass contents increased gradually with the anther-derived embryos development as a whole. Soluble sugars, soluble protein and nucleic acid were closely related to the development and maturation of embryos, changing significantly in metabolism at the important development turning points. Soluble sugar, fructose and starch contents had the same change trend. The two accumulation peaks appeared at globular and cotyledon stages. During the development of loquat anther embryos, the dynamics of protein synthesis were roughly in "S" shape. The two accumulation peaks appeared at globular and cotyledon stage, respectively, in accordance with the change of sugar and starch. Alkaline protein contents were higher than acidic protein contents. Alkaline protein contents and total soluble protein contents had the same change trend during the development process. DNA contents and total nucleic acid contents had the same change trend during the development of anther-derived embryos. The DNA synthesis peaks appeared at the embryogenic callus stage. RNA contents were very low at embryogenic callus stage and cotyledon stage, while DNA was actively synthesized at the two stages.Key words: Eriobotrya japonica, anther culture, embryos, metabolic substances

Analysis on the Risk and Supervision of P2P Online Financing Platforms in China

Microcredit is a vital breakthrough to solve the financial problems of low-income groups and small and medium-sized enterprises, while traditional microfinance providers can only meet a small proportion of their capital needs. By using internet technology, P2P online financing extends the innovative development of microcredit with the aim of solving traditional micro-credit problems. This paper mainly explores the existing online financing operation model of P2P in China, and summarizes the relevant problems, such as low entry barriers for P2P online financing enterprises and lack of supervision, Lack of verification on the qualification of borrowers and poor management of the platform, imperfect information revealed or providing false information by platform, etc. Finally, the article put forward some suggestions concerning the healthy development for the P2P online financing platform, including the establishing entry audit system and strengthening the supervision of the P2P platform, strengthening the management of borrowers and improving the credit collection system, and strengthening the disclosure of information by platform

On the Abundance of Potassium in Metal-Poor Stars

Based on extensive statistical-equilibrium calculations, we performed a non-LTE analysis of the K {\sc i} 7699 equivalent-width data of metal-deficient stars for the purpose of clarifying the behavior of the photospheric potassium abundance in disk/halo stars. While the resulting non-LTE abundance corrections turned out to be considerably large, amounting to 0.2--0.7 dex, their effect on the [K/Fe] vs. [Fe/H] relation is not very important, since these corrections do not show any significant metallicity dependence. Hence, we again confirmed the results of previous LTE studies, that [K/Fe] shows a gradual systematic increase toward a lowered metallicity up to [K/Fe] $\sim$ 0.3--0.5 at [Fe/H] $\sim$ -1 to -2, such as in the case of $\alpha$ elements.Comment: 16 pages (including 2 tables and 4 figures), to appear in PASJ, Vol.54, No.2 (using the style file pasj00.cls

EFFECTS OF LONG-TERM TAI CHI EXERCISE ON BALANCE CONTROL IN OLDER ADULTS

This study assessed the static and dynamic balance control of older adults who have 10 years of Tai Chi exercise experience and compared their characteristics with their sedentary counterparts. The abilities were measured using methods: single-leg stance times with eyes open and closed; sway of center of pressure (COP) during static standing with eyes open/closed, and leaning the body in three specific directions. Compared with control group, 1) Tai Chi Group showed longer single-leg stance times with eyes open and closed, 2) slower sway velocity of COP in mediolateral and anterioposterior directions and shorter sway distance in both directions, and 3) shorter total, anterioposterior, and mediolateral routes and shorter time spent during the dynamic balance test. Long-term Tai Chi exercise improves the balance ability, especially the dynamic balance, of older adults

Construction of interval wavelet based on restricted variational principle and its application for solving differential equations

Based on restricted variational principle, a novel method for interval wavelet construction is proposed. For the excellent local property of quasi-Shannon wavelet, its interval wavelet is constructed, and then applied to solve ordinary differential equations. Parameter choices for the interval wavelet method are discussed and its numerical performance is demonstrated

Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells

AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of expressing S or S2S proteins stably. METHODS: Segments of S, preS2-S, preS1-preS2-S genes of Hepatitis B virus were amplified by routine PCR and preS1-S fragment was amplified by Over-Lap Extension PCR. The amplified segments were cleaved with restricted endonuclease Hind III/Not I followed by ligation with pRc/CMV, or BamHI/EcoR I followed by ligation with pSG5UTPL/Flag. After the plasmid vectors were cleaved with the correspond enzymes, the amplified segments were inserted into pRc/CMV or pSG5UTPL/Flag plasmid vectors with T4 DNA ligase. KOZAK sequence was added before the initial ATG code of each fragment using specific primer. The inserted segments in the recombinant plasmids were sequenced after subcloning. BALB/c mice myeloma cells (SP2/0 cell line) were transfected with the recombinant plasmids. The expressions of the different recombinants were compared by Western-blot, using a monoclonal anti-HBs antibody as the primary antibody and peroxidase-labeled multi-linker as the secondary. Stable SP2/0-pRc/CMV-S or SP2/0-pRc/CMV-MS clones were established through clone screening with G418. RESULTS: Fragments with anticipated size were harvested after PCR. After recombination and screening, the sequences of the inserted segments in the recombinants were confirmed to be S, preS2S, preS1-preS2S and preS1S encoding genes, determined by sequencing. The results of Western-blot hybridization were positive for the anticipated proteins. Among them, pRc/CMV-S or pRc/CMV-MS demonstrated the highest expressing their respective antigen. CONCLUSION: Eight recombinant plasmids expressing S, M, L or preS1S proteins are obtained. For hepatitis surface antigen expression in eukaryotic cells, the vector pRc/CMV is superior to pSG5UTPL/Flag, and pRc/CMV-S and pRc/CMV-MS are the most efficient in the pRc/CMV clones. SP2/0 cells stably expressing HBsAg are established, and may be used as target cells for evaluating the CTL activity of a DNA vaccine in vitro

1,4-Dibromo-2,5-dimeth­oxy­benzene

The asymmetric unit of the title compound, C8H8Br2O2, contains one half-mol­ecule, the complete mol­ecule being generated by inversion symmetry