Identification of stromal cells in spleen which support myelopoiesis

Stromal cells in spleen organize tissue into red pulp, white pulp and marginal zone, and also interact with hematopoietic cells to regulate immune responses. This study has used phenotypic information of a previously described spleen stromal cell line called 5G3, which supports restricted hematopoiesis in vitro, to identify an equivalent stromal cell subset in vivo and to test its capacity to support hematopoiesis. Using stromal cell fractionation, phenotypic analysis, as well as cell growth and hematopoietic support assays, the Sca-1+gp38+Thy1.2+CD29+CD51+ fraction of spleen stroma has been identified as an equivalent stromal subset resembling the 5G3 cell counterpart. While heterogeneity may still exist within that subset, it has been shown to have superior hematopoietic support capacity compared with the 5G3 cell line, and all other spleen stromal cell fractions tested.This work was supported by project grants to HO from the Australian Research Council (#DP130101703) and the National Health and Medical Research Council of Australia (#585443). HL was supported by an Australian National University Postgraduate Scholarship

Identification of Frequency Ranges for Subharmonic Oscillations in a Relay Feedback System

This paper examines the behaviour of a single loop relay feedback system (RFS) which is driven by an external signal. It is well known that such a RFS exhibits a variety of oscillation patterns including forced and subharmonic oscillations (SO). This paper focuses on the conditions for SO. It will be shown that for an external signal with a fixed amplitude, it is possible for SO with different orders to occur simply by changing the frequency of the external signal. Similarly, for an external signal with a fixed frequency, it is possible for SO with different orders to occur when the amplitude of the external signal is varied. The conditions under which these different scenarios will occur are explored. An analysis of these conditions identifies the frequency ranges where certain orders of SO are possible for a given amplitude of the external signal. The effects of the initial conditions on the SO are illustrated and discussed. Simulation studies are presented to illustrate the result

A prediction model of the depth-of-discharge effect on the cycle life of a storage cell

Cycle life requirements are very high for batteries used in aerospace applications in low Earth orbit. The data base required to establish confidence in a particular cell design is thus both extensive and expensive. Reliable accelerated cycle life testing and performance decay modeling represent attractive alternatives to real-time tests of cycle life. In light of certain long-term cycle life test results, this paper examines a very simple performance decay model developed earlier. Application of that model to available data demonstrates a rigid relationship between a battery's expected cycle life and the depth of discharge of cycling. Further, modeling analysis of the data suggests that a significantly improved cycle life can be obtained with advanced components, materials, and designs; and that cycle life can be reliably predicted from the results of accelerated testing

Modelling and Parameter Identification Using Reduced I-V Data

No abstract available