Identification of contigs containing genes of interest and verification of the physical map.

*<p>The positive clones of the genes were identified previously by screening the physical map source BIBAC library using overgo probes designed from the gene sequences <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033644#pone.0033644-Rong2" target="_blank">[34]</a>. The clones not bolded in the table indicate singletons.</p

Summary of the allotetraploid Upland cotton genome physical map.

<p>Summary of the allotetraploid Upland cotton genome physical map.</p

An A-subgenome BIBAC contig of the Upland cotton physical map.

<p>The contig consists of 187 BIBACs, spanning 6,380 kb in physical length or approximately 13 cM in genetic distance.</p

A new cyclic dipeptide from cultures of <i>Coprinus plicatilis</i>

<p>Three cyclic dipeptides (<b>1–3</b>), including one new compound (<b>1</b>) were isolated from cultures of the basidiomycetes <i>Coprinus plicatilis</i>. Their structures were elucidated by spectroscopic methods, including extensive 2D NMR techniques. At the same time, all compounds were tested for their cytotoxicities against five human cancer cell lines.</p

Catalytic Enantioselective and Divergent Total Synthesis of (+)-10-Oxocylindrocarpidine, (+)-Cylindrocarpidine, (−)‑<i>N</i>‑Acetylcylindrocarpinol, and (+)-Aspidospermine

The catalytic enantioselective and divergent total syntheses of Aspidosperma alkaloids (+)-10-oxocylindrocarpidine <b>7</b>, (+)-cylindrocarpidine <b>1</b>, (−)-<i>N</i>-acetylcylindrocarpinol <b>6</b>, and (+)-aspidospermine <b>8</b> have been accomplished in 11 steps from a common precursor (<b>15</b>) on the basis of a highly concise route. The route features three metal-catalyzed reactions, including the key Pd-catalyzed decarboxylative asymmetric allylation of carbazolones developed in our laboratory. Our syntheses, using a combination of C–H activation, enantioselective catalysis, and collective synthesis, represent the first total synthesis of 10-oxocylindrocarpidine and the first asymmetric total synthesis of cylindrocarpidine and <i>N</i>-acetylcylindrocarpinol

Enantioselective Total Synthesis of (−)-Limaspermidine and Formal Synthesis of (−)-1-Acetylaspidoalbidine

Evolution of the synthetic strategy that culminated in the first asymmetric total synthesis of the Aspidosperma alkaloid limaspermidine is described. The successful enantioselective route to (−)-limaspermidine proceeds in 10 steps and with the isolation of only six intermediates using a Pd-catalyzed enantioselective decarboxylative allylation we have recently developed. This first enantioselective synthesis of (−)-limaspermidine establishes unambiguously its absolute configuration and allows the first asymmetric formal total synthesis of the Aspidoalbine alkaloid (−)-1-acetylaspidoalbidine

Genetic relationships between <i>Cicer</i> sp.

<p>(A) A principal coordinate (PCo) plot of pairwise individual genetic distances among four wild <i>Cicer</i> species and domesticated genotypes. (B) Estimated population structure based on allele frequency variation of SNPs markers. Each genotype is represented by a vertical bar, which is partitioned into <i>K-</i>colored components representing the ancestry fractions in <i>K</i> = 5 clusters. Individual genotypes are ordered by species (labeled below).</p

Wild <i>Cicer reticulatum</i> accession Cr25.

<p>(<b>A</b>) The habitat near Midyat, Turkey where Cr25 was sampled, photographed in May 2009. (<b>B</b>) A mature wild <i>C</i>. <i>reticulatum</i> plant in the source population of Cr25 on the occasion of seed sampling, June 2007. (<b>C</b>) Growth habit and flower colour of Cr25 as compared with a typical <i>C</i>. <i>reticulatum</i> accession (Cr2). (<b>D</b>) The seed germination pattern of Cr25. Right, intact 5 months old seeds. Left, control (scarified) 5 month old seeds. (<b>E</b>) A perennial <i>Cicer</i> sp. with white flowers. Photograph taken on Mt. Kizilnora, Uzbekistan, July 2005.</p

Anisucoumaramide, a Bioactive Coumarin from <i>Clausena anisum-olens</i>

A new coumarin, anisucoumaramide (<b>1</b>), and a new δ-truxinate derivative, anisumic acid (<b>2</b>), were isolated from <i>Clausena anisum-olen</i>s. Their structures were elucidated from extensive NMR and MS data. The absolute configurations of the coumarins were assigned using the experimental and calculated electronic circular dichroism data. Anisucoumaramide (<b>1</b>) represents the first example of a naturally occurring coumarin of which the terpenoidal side chain does not comply with the biosynthesis isoprene rule due to the presence of an unprecedented acetamido motif directly connected with the terpenoidal side chain. The δ-truxinate derivative was isolated from <i>Clausena</i> species for the first time. Compound <b>1</b> showed high selectivity for the MAO-B isoenzyme and inhibitory activity in the nanomolar range. Putative biosynthesis pathways toward <b>1</b> and <b>2</b> are proposed

Genetic relationships between wild and domesticated <i>Cicer</i> sp.

<p>(<b>A</b>) A principal coordinate (PCo) plot of pairwise individual genetic distances among wild <i>Cicer reticulatum</i> and domesticated <i>C</i>. <i>arietinum</i> genotypes. (<b>B</b>) Estimated population structure based on allele frequency variation of SNPs markers. Each genotype is represented by a vertical bar, which is partitioned into <i>K-</i>colored components representing the ancestry fractions in <i>K</i> = 3 clusters. Individual genotypes are ordered by species (labeled below).</p