<p>Three cyclic dipeptides (<b>1โ3</b>), including one new compound (<b>1</b>) were isolated from cultures of the basidiomycetes <i>Coprinus plicatilis</i>. Their structures were elucidated by spectroscopic methods, including extensive 2D NMR techniques. At the same time, all compounds were tested for their cytotoxicities against five human cancer cell lines.</p