Structure and substrate selectivity of the 750-kDa α6β6 holoenzyme of geranyl-CoA carboxylase.

Geranyl-CoA carboxylase (GCC) is essential for the growth of Pseudomonas organisms with geranic acid as the sole carbon source. GCC has the same domain organization and shares strong sequence conservation with the related biotin-dependent carboxylases 3-methylcrotonyl-CoA carboxylase (MCC) and propionyl-CoA carboxylase (PCC). Here we report the crystal structure of the 750-kDa α6β6 holoenzyme of GCC, which is similar to MCC but strikingly different from PCC. The structures provide evidence in support of two distinct lineages of biotin-dependent acyl-CoA carboxylases, one carboxylating the α carbon of a saturated organic acid and the other carboxylating the γ carbon of an α-β unsaturated acid. Structural differences in the active site region of GCC and MCC explain their distinct substrate preferences. Especially, a glycine residue in GCC is replaced by phenylalanine in MCC, which blocks access by the larger geranyl-CoA substrate. Mutation of this residue in the two enzymes can change their substrate preferences

Quakes in Solid Quark Stars

A starquake mechanism for pulsar glitches is developed in the solid quark star model. It is found that the general glitch natures (i.e., the glitch amplitudes and the time intervals) could be reproduced if solid quark matter, with high baryon density but low temperature, has properties of shear modulus \mu = 10^{30~34} erg/cm^3 and critical stress \sigma_c = 10^{18~24} erg/cm^3. The post-glitch behavior may represent a kind of damped oscillations.Comment: 11 pages, 4 figures (but Fig.3 is lost), a complete version can be obtained by http://vega.bac.pku.edu.cn/~rxxu/publications/index_P.htm, a new version to be published on Astroparticle Physic

Catastrophic disassembly of actin filaments via Mical-mediated oxidation.

Actin filament assembly and disassembly are vital for cell functions. MICAL Redox enzymes are important post-translational effectors of actin that stereo-specifically oxidize actin's M44 and M47 residues to induce cellular F-actin disassembly. Here we show that Mical-oxidized (Mox) actin can undergo extremely fast (84 subunits/s) disassembly, which depends on F-actin's nucleotide-bound state. Using near-atomic resolution cryoEM reconstruction and single filament TIRF microscopy we identify two dynamic and structural states of Mox-actin. Modeling actin's D-loop region based on our 3.9 Å cryoEM reconstruction suggests that oxidation by Mical reorients the side chain of M44 and induces a new intermolecular interaction of actin residue M47 (M47-O-T351). Site-directed mutagenesis reveals that this interaction promotes Mox-actin instability. Moreover, we find that Mical oxidation of actin allows for cofilin-mediated severing even in the presence of inorganic phosphate. Thus, in conjunction with cofilin, Mical oxidation of actin promotes F-actin disassembly independent of the nucleotide-bound state

High Density Effective Theory Confronts the Fermi Liquid

The high density effective theory recently introduced by Hong and Hsu to describe ultradense relativistic fermionic matter is used to calculate the tree-level forward scattering amplitude between two particles at the Fermi surface. While the direct term correctly reproduces that of the underlying gauge theory, the exchange term has the wrong sign. The physical consequences are discussed in the context of Landau's theoretical description of the Fermi liquid.Comment: 15 pages, 2 figures; conclusion expanded, reference adde

Preparation and in vitro characterization of scaffolds of poly(L-lactic acid) containing bioactive glass ceramic nanoparticles

Porous nanocomposite scaffolds of poly(L-lactic acid) (PLLA) containing different quantities of bioactive glass ceramic (BGC) nanoparticles (SiO2:CaO:P2O5 ! 55:40:5 (mol)) were prepared by a thermally induced phase-separation method. Dioxane was used as the solvent for PLLA. Introduction of less than 20 wt.% of BGC nanoparticles did not remarkably affect the porosity of PLLA foam. However, as the BGC content increased to 30 wt.%, the porosity of the composite was observed to decrease rapidly. The compressive modulus of the scaffolds increased from 5.5 to 8.0 MPa, while the compressive strength increased from 0.28 to 0.35 MPa as the BGC content increased from 0 to 30 wt.%. The in vitro bioactivity and biodegradability of nanocomposites were investigated by incubation in simulated body fluid (SBF) and phosphate-buffered saline, respectively. Scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction were employed to monitor the surface variation of neat PLLA and PLLA/ BGC porous scaffolds during incubation. PLLA/(20 wt.%)BGC composite exhibited the best mineralization property in SBF, while the PLLA/(10 wt.%)BGC composite showed the highest water absorption ability.This work was financially supported by FCT Grant for postdoctoral research (SFRH/BPD/25828/2005), and by the Projects POCTI/FIS/61621/2004 and PTDC/QUI/69263/2006. The authors also would like to acknowledge Dr. Aixue Liu, Changchun Institute of Applied Chemistry, for his help in characterization of composite material

Spin correlated interferometry for polarized and unpolarized photons on a beam splitter

Spin interferometry of the 4th order for independent polarized as well as unpolarized photons arriving simultaneously at a beam splitter and exhibiting spin correlation while leaving it, is formulated and discussed in the quantum approach. Beam splitter is recognized as a source of genuine singlet photon states. Also, typical nonclassical beating between photons taking part in the interference of the 4th order is given a polarization dependent explanation.Comment: RevTeX, 19 pages, 1 ps figure, author web page at http://m3k.grad.hr/pavici

Corrosion behaviour of nitrided ferritic stainless steels for use in solid oxide fuel cell devices

Plasma nitriding was applied to ferritic stainless steel substrates to improve their performances as interconnects for solid oxide fuel cell devices. The samples underwent electrical conductivity test and SEM/EDS, TEM/EDS, environmental-SEM analyses. The first stages of corrosion were recorded in-situ with the e-SEM. Nitriding is effective in limiting the undesired chromium evaporation from the steel substrates and accelerates the corrosion kinetics, but its influence of the electrical conductivity is ambiguous. No intergranular corrosion is found in the steel substrate after long time operation. Nitriding helps commercially competitive porous coating to improve chromium retention properties of metal interconnects